Multi-functional

exotic bioactive
protein to defend
& restore skin
from photo-aging

Aging Redefined
The concept of aging goes beyond
chronology
Intrinsic and extrinsic factors e.g.
genetics and lifestyle have a strong
influence on aging
UV exposure or photo-damage
profoundly affects the aging process
Symptoms of photo-aging are visible
long before signs of chronological aging
UV radiation directly alters DNA and can
induce lesions as well as single and
double strand breaks

A Refresher on DNA

DNA serves as the instruction manual for

the development & function of all living
organisms
It consists of two strands
(polynucleotides) that are composed of
smaller nucleotide units.
Each nucleotide contains a sugar group
(deoxyribose), a phosphate group and a
nucleobase (base) which are linked
together covalently
4 bases join strands together via 4 bases:
Adenine (A) always pairs with
Thymine (T)
Guanine (G) always pairs with
Cytosine (C)
The strands are then wound into a helical
structure
3

DNA Lesions

Form on single strands of DNA and

includes thymine dimers
Lesions are the most cytotoxic and
typically occur between adjacent
pyrimidine bases
Although only a fraction of dimers
results in mutation, all are capable
of blocking gene transcription and
DNA replication which leads to
cellular senescence

Double Strand Breaks

Both strands in the double helix

are severed
Among the types of damage that
occur, DSBs are considered to be
the most dangerous since they
can lead to a loss of genetic
information
When DSBs occur H2AX histones
are phosphorylated as a means to
signal damage
This is the first step in DNA
repair & induces the expression
of p53

The Guardian of the Genome

p53 is a nuclear protein that regulates

cell division & plays a crucial role in
cellular response to damage
It prevents mutations by binding to
DNA to induce senescence and
activate repair or initiate apoptosis
(cell death) to prevent mutated or
damaged DNA from dividing
In healthy cells, it is present at very
low levels yet after damage there is a
significant increase in the expression
of this protein
p53 levels return to normal as cellular
health is restored

DNA
Damage

p53

Cell Cycle Arrest

Apoptosis

DNA Repair

Cell Cycle Restart

Death & elimination

of damaged cell

Cellular & Genetic Stability

Response To Chronic Damage

Cellular cohesion is disrupted

by abnormally shaped & sized
keratinocytes

Stratum corneum & basement

membrane thicken along with
increases in melanocyte
proliferation & melanogenesis

Dermal changes include

decreases in collagen synthesis,
increases in MMPs & altered
elastin deposits

What Does This Mean?

UV radiation accelerates the pace of aging
Prolonged exposure causes:
Wrinkles & fine lines
Sagging skin
Rough, leathery texture
Dark spots & discolorations
Broken capillaries

Image Source: Huffpost.com

Our Natural Defense for UV Radiation

Defense mechanisms include UV

absorbing pigments (melanin), protective
enzymes and oxidative scavengers
The DNA repair process is highly
conserved to address specific damage,
Mechanisms are not always sufficient for
repair & prevention is typically
considered the best course of action

Introducing
Baobab
Tein NPNF

Baobab The Iconic Tree of Africa

Latin name: Adansonia digitata
Other common names: Tree of Life, Upsidedown tree, Lemonade tree, Monkey-bread
tree
Baobab is the largest succulent plant in the
world
Most renown for affording protection and
water:
The tree thrives into old age and often lives
more than 2,000 years
In African folklore, Baobab is steeped in
mystique and legend:
Water infused with Baobab seeds gives
strength and good health
Baobab seeds are carried to a new
homestead to bring it good fortunes
11

Baobab Community Benefits & Sustainability

Our Partner in Trade:

Community Benefits:

A local business providing baobab to food and cosmetic industries

in Africa and US

Sourced directly from local communities that live in harvest areas

Harvest areas are purposefully chosen to be in the underdeveloped
areas to bring much needed economic opportunity to local families

Sustainable Harvesting:

Collected to avoid damage to trees

Annual baobab planting program
Research & monitoring program ensures health of baobab trees in
harvest areas
Educational programs and websites about baobab and their ecosystem
Our partner is dedicated to promoting the responsible and
sustainable development of African businesses offering natural
products

12

Bioactive Protein with Multi-Functional Benefits

DEFEND
From UV stress by limiting cellular photo-aging

RESTORE
Skin vitality by significantly reducing DNA damage
following UV exposure

MAINTAIN
Healthy, supple skin with nourishing amino acids

13

Baobab Tein NPNF Amino Acid composition

Amino Acids

Restore
38%

Maintain
38%

Defend
24%

Arginine
Alanine
Aspartic Acid
Cysteine
Glutamic Acid
Glycine
Histidine
Leucine
Lysine
Methionine
Phenylalanine
Proline
Serine

Maintain Defend Restore

X

X
X

X
X

X
X
X

X
X

X
X
X
X

X
X
X

X
X
X
X

Baobab Tein NPNF contains amino acids that

help defend, restore & maintain skin health

Benefits
Testing

Summary of Benefits Tested

Defend

Restore

Maintain

Pre-treatment
significantly
decreases cellular
photo-damage

Post-treatment
significantly
reduces cellular
photo-aging

Supports viability
of normal,
healthy cells

Improves barrier
function to
protect & lock-in
moisture

Speeds damage
recovery

Beneficial amino
acids support
health

Provides
antioxidant
protection

Enhances cellular
vitality

Helps skin to
retain moisture

Baobab Tein NPNF - Gene Expression

DNA microarray data* shows Baobab Tein NPNF up
regulates expression of genes involved in DNA
repair including the p53 & histone H2AX
Works to support skins natural response to damage

*0.1% Baobab Tein NPNF was used to treat

fibroblast cells for 24 hours before the study

17

DEFEND

Defend Testing Performed

Study 1: Photo-Damage
Study 2: DNA Double
Strand Breaks
Study 3: Enhance Barrier
Function

Study 4: Antioxidant
Protection

19

Study 1: Defend Photo-Damage

Non-UV Exposed

0.25% Baobab Tein NPNF Treatment

Pre-treatment with Baobab Tein NPNF significantly limits

damage that causes cell senescence & photo-aging
Fibroblasts were treated with test materials and then exposed to UV
20

UV-Exposed No Treatment

Study 2: Defend DNA Double Strand Breaks

Non-UV Exposed

0.25% Baobab Tein NPNF Treatment

Pre-treatment with 0.25% Baobab Tein NPNF helped

significantly prevent H2AX phosphorylation which is
indicative of double strand breaks
Fibroblasts were treated with test materials and then exposed to UV
21

UV-Exposed No Treatment

Study 3: Defend Enhance Barrier Function

Baobab Tein NPNF
improves the integrity
of the stratum corneum
Significantly increases
barrier function to lock
in moisture
The defensive barrier
acts as a shield to
protect the skin from
foreign substances
Baobab Tein NPNF helps minimize TEWL to
enhance skins natural barrier & lock in moisture
6 female subjects (ages 22 59) applied creams twice daily for 14 days

Study 4: Defend Antioxidant Protection

Baobab Tein NPNF
delivers a dosedependent antioxidant
capacity
1.0% Baobab Tein NPF
is equivalent to 181
M of Trolox

Baobab Tein NPNF exhibits antioxidant activity

to help shield from ROS damage

RESTORE

Restore Testing Performed

Study 5: Photo-Aging
Study 6: DNA Double
Strand Breaks
Study 7: Cellular Health
After Damage

Study 8: Repair of DNA

Lesions

25

Study 5: Restore Photo-Aging

Non-UV Exposed

0.25% Baobab Tein NPNF Treatment

After treatment, Baobab Tein NPNF significantly limited cell

senescence to restore health & reduce photo-aging
Fibroblasts were exposed to UV and then treated with test materials
26

UV-Exposed No Treatment

Study 6: Restore DNA Double Strand Breaks

Non-UV Exposed

0.25% Baobab Tein NPNF Treatment

Baobab Tein NPNF helped significantly repair damage after

UV by limiting H2AX phosphorylation to improve DNA integrity
and optimize cellular health
Fibroblasts were exposed to UV and then treated with test materials
27

UV-Exposed No Treatment

Study 7: Restore Cellular Health After Damage

p53 levels in healthy, non-UV
exposed cells is much better
than untreated, UV exposed
cells
Baobab Tein NPNF
significantly improved p53
levels compared to
untreated cells
Data indicates Baobab Tein
NPNF speeds damage
recovery

Baobab Tein NPNF shown to help restore cellular

health following UV damage
Keratinocytes were exposed to UV and then treated with test materials
28

Study 8: Restore Repair of DNA Lesions

Baobab Tein NPNF shown
to significantly reduce
thymine-dimer formation to
help repair DNA damage
Coupled with the p53 data,
this indicates Baobab Tein
NPNF helps counteract
photo-damage that can
contribute to aging

Baobab Tein NPNF shown to support integrity of DNA

following UV induced damage
Keratinocytes were exposed to UV and then treated with test materials
29

MAINTAIN

Maintain Testing Performed

Study 9: Improves
Viability for Better Health

Study 10: Improves

Moisturization

31

Study 9: Maintain Improves Cellular Health & Proliferation

Baobab Tein
NPNF significantly
increased viability
of healthy
keratinocytes &
fibroblasts

Baobab Tein NPNF improves vitality to help

maintain skin health
Cells were either treated with Baobab Tein NPNF or left untreated

Study 10: Maintain Improves Moisturization

Baobab Tein NPNF helps maintain skin

health & provides continued moisturization
8 female subjects (ages 22 59) applied creams twice daily for 14 days

Baobab Tein
NPNF
improves skin
integrity to
significantly
improve
moisturization
Moisturization
is a reflection of
skin health

Baobab Tein NPNF Summary

INCI:
Hydrolyzed Adansonia Digitata
Seed Extract

Suggested Applications:
Leave-on products:
Night & Day Creams
Day Creams & Lotions with
SPF
BB & CC Creams
Toners
Serums
Body Lotions
Sunscreens & After-Sun
Products

Multifunctional protein provides 3-in-1

benefits to target photo-aging:
Defends from UV stress by limiting
cellular photo-aging
Restores skin vitality by significantly
reducing DNA damage following UV
exposure
Maintains healthy, supple skin with
nourishing amino acids

Suggested Use Level:

25%

34

Thank You!
Elzbieta Kasprzyk
Elzbieta.Kasprzyk@tri-k.com
www.tri-k.com
info@tri-k.com

Visit us at Booth # 6F60