ORIGINAL RESEARCH
ABSTRACT:
3.CIAD-Unidad Delicias Av
4ta Sur 3820, Fracc.
Vencedores del Desierto .
Delicias, Chihuahua 33089
Keywords:
Mexico
C. gloeosporioides, C. acutatum, Pectate Lyase
Corresponding author:
Calderon-Santoyo M
Web Address:
http://jresearchbiology.com/
documents/RA0388.pdf
Article Citation:
Berecochea-Lpez A, Ragazzo-Snchez JA, Allende-Molar R, Avila-Quezada GD and
Calderon-Santoyo M.
Colletotrichum gloeosporioides from Mango Ataulfo: morphological, physiological,
genetic and pathogenic aspects.
Journal of Research in Biology (2015) 5(2): 1641-1647
Dates:
Received: 18 Oct 2013 Accepted: 26 Oct 2013 Published: 18 Mar 2015
This article is governed by the Creative Commons Attribution License (http://creativecommons.org/
licenses/by/4.0), which gives permission for unrestricted use, non-commercial, distribution and
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www.jresearchbiology.com
Fungal isolates
Glomerella
and
Nayarit.
cingulata
(Stoneman)
Spauld.
provides
the
important
information
concerning
et al., 2000).
2003).
plates
containing
PDA
to
study
morphological
pathogenicity process.
1642
was recorded for each isolate. The size, shape and colour
described above.
Sensitivity to benomyl
-1
-1
NaCl, 10 mmol L
-1
-1
(10 mmol L
Tris-HCl, pH 8, 1 mmol L
-1
EDTA).
-1
gel RED, and the bands were visualized under a Gel Doc
2000
DNA
Nanodrop
UV
transilluminator
concentration
was
(Bio-Rad).
quantied
using
The
a
primers
CgInt
(5-
ITS4
4,5-
amplications
initial
GGCCTCCCGCCTCCGGGCGG-3)
were
performed
with
and
an
unsaturated
galacturonide
product
of
1643
protein
by
at 235nm.
extract
concentrated
media,
followed
isolates,
morphological
and
growth
characteristics
Six isolates showing macroscopic characteristics
Growth rates
Isolates
M2V
Length
10.39
Width
2.75
Isolates
SA
12.42
2.77
F1
11.00
3.34
A71 NB
DG
11.24
16.14
3.21
4.02
GAQ37
9.78
3.40
M2V
SA
F1
A71NB
DG
GAQ37
Figure 2. Agarose gel showing the DNA fragment amplified from the oligonucleotides CgInt and ITS4 for the
Colletotrichum gloeosporioides. M: Molecular weight
marker 100 pb, 1: F1, 2: M2V, 3: SA, 4: DG, 5: GAQ37,
6: A71 B, 7: Positive control Colletotrichum gloeosporioides (Yucatn).
Journal of Research in Biology (2015) 5(2): 1641-1647
M2V
SA
F1
A71NB
DG
GAQ37
1
1
1
1
1
1
0
0
0
1
0
0
0.6 g/L
Benomyl
0
0
0
1
0
0
1.2 g/L
Benomyl
0
0
0
0
0
0
M2V
SA
F1
DG
234.7a
238.0a
102.7a
387.69a
412.1b
410.96b
271.6b
416.13a
acutatum.
Sensitivity to benomyl
Colletotrichum strains were classified as C.
Molecular identification
According to the use of the primers CgInt and
40kD
C
Figure 3. Western blot of extracts from Colletotrichum
spp. isolates culture with and without mango cells.
1645
82(6): 596-605.
Mills PR, Brown AE and Sreenivasaprasad S. 1992.
CONCLUSION
The
morphological
and
physiological
to
classify
them
up
to
the
species
Detection
and
differentiation
gloeosporioides
isolates
of
Colletotrichum
using
PCR,
FEMS
pathogenicity
of
Colletotrichum.
sp.cv
Ataulfo
2p.
Prusky D, McEvoy JL, Leverentz B and Conway WS.
2001. Local modulation of host pH by Colletotrichum
species as a mechanism to increase virulence, Molecular
Plant-Microbe Interactions. 14(9): 1105-1113.
Sanders GM and Korsten L. 2003. A comparative
its
application
for
rapid
plant
disease
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Adaskaveg JE and Hartin RJ. 1997. Characterization
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Biology,
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and
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D.
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2008.
Quijano-Ramayo
PCR-based
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and
Katan
and
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E.
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and
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1647