References:

1) Han, Y. W., Shi, W., Huang, G. T. J., Haake, S. K., Park, N. H., Kuramitsu, H., & Genco, R. J. (2000).
Interactions between periodontal bacteria and human oral epithelial cells: Fusobacterium nucleatum
adheres to and invades epithelial cells. Infection and Immunity, 68(6), 3140-3146.
2) Bolstad, A. I., Jensen, H. B., & Bakken, V. (1996). Taxonomy, biology, and periodontal aspects of
Fusobacterium nucleatum. Clinical microbiology reviews,9(1), 55-71.
3) Rickard, A. H., Gilbert, P., High, N. J., Kolenbrander, P. E., & Handley, P. S. (2003). Bacterial
coaggregation: an integral process in the development of multi-species biofilms. Trends in
microbiology, 11(2), 94-100.
4) Karatan, E., Duncan, T. R., & Watnick, P. I. (2005). NspS, a predicted polyamine sensor, mediates
activation of Vibrio cholerae biofilm formation by norspermidine. Journal of bacteriology, 187(21),
7434-7443.
5) Rickard, A. H., Gilbert, P., High, N. J., Kolenbrander, P. E., & Handley, P. S. (2003). Bacterial
coaggregation: an integral process in the development of multi-species biofilms. Trends in
microbiology, 11(2), 94-100.
6) Bradshaw, David J., et al. "Role of Fusobacterium nucleatum and coaggregation in anaerobe
survival in planktonic and biofilm oral microbial communities during aeration." Infection and
immunity 66.10 (1998): 4729-4732.
7) Flemming, H. C., & Wingender, J. (2010). The biofilm matrix. Nature Reviews Microbiology, 8(9),
623-633.
8) Teles, F. R., Teles, R. P., Uzel, N. G., Song, X. Q., Torresyap, G., Socransky, S. S., & Haffajee, A. D.
(2012). Early microbial succession in redeveloping dental biofilms in periodontal health and
disease. Journal of periodontal research, 47(1), 95-104.
9) Han, Y. W., Shi, W., Huang, G. T. J., Haake, S. K., Park, N. H., Kuramitsu, H., & Genco, R. J. (2000).
Interactions between periodontal bacteria and human oral epithelial cells: Fusobacterium nucleatum
adheres to and invades epithelial cells. Infection and Immunity, 68(6), 3140-3146.
10) Hajishengallis, G., Liang, S., Payne, M. A., Hashim, A., Jotwani, R., Eskan, M. A., ... & Curtis, M.
A. (2011). Low-abundance biofilm species orchestrates inflammatory periodontal disease through
the commensal microbiota and complement. Cell host & microbe, 10(5), 497-506.
11) Patel, C. N., Wortham, B. W., Lines, J. L., Fetherston, J. D., Perry, R. D., & Oliveira, M. A. (2006).
Polyamines are essential for the formation of plague biofilm. Journal of bacteriology, 188(7), 23552363.
12) Wortham, B. W., Oliveira, M. A., Fetherston, J. D., & Perry, R. D. (2010). Polyamines are required
for the expression of key Hms proteins important for Yersinia pestis biofilm formation. Environmental
microbiology, 12(7), 2034-2047.
13) Karatan, E., & Michael, A. J. (2013). A wider role for polyamines in biofilm
formation. Biotechnology letters, 35(11), 1715-1717.
14) Karatan, E., Duncan, T. R., & Watnick, P. I. (2005). NspS, a predicted polyamine sensor, mediates
activation of Vibrio cholerae biofilm formation by norspermidine. Journal of bacteriology, 187(21),
7434-7443.
15) Burrell, M., Hanfrey, C. C., Murray, E. J., Stanley-Wall, N. R., & Michael, A. J. (2010). Evolution
and multiplicity of arginine decarboxylases in polyamine biosynthesis and essential role in Bacillus
subtilis biofilm formation. Journal of Biological Chemistry, 285(50), 39224-39238.
16) Hobley, L., Kim, S. H., Maezato, Y., Wyllie, S., Fairlamb, A. H., Stanley-Wall, N. R., & Michael, A.
J. (2014). Norspermidine is not a self-produced trigger for biofilm disassembly. Cell, 156(4), 844854.
17) Kapatral, V., Anderson, I., Ivanova, N., Reznik, G., Los, T., Lykidis, A., ... & Overbeek, R. (2002).
Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC
25586. Journal of Bacteriology, 184(7), 2005-2018..
18) Goytia, M., Dhulipala, V. L., & Shafer, W. M. (2013). Spermine impairs biofilm formation by
Neisseria gonorrhoeae. FEMS microbiology letters, 343(1), 64-69.

19) Kashiwagi, K., Suzuki, T., Suzuki, F., Furuchi, T., Kobayashi, H., & Igarashi, K. (1991).
Coexistence of the genes for putrescine transport protein and ornithine decarboxylase at 16 min on
Escherichia coli chromosome. Journal of Biological Chemistry, 266(31), 20922-20927.
20) Kanehisa, M., Goto, S., Sato, Y., Kawashima, M., Furumichi, M., and Tanabe, M.; Data,
information, knowledge and principle: back to metabolism in KEGG. Nucleic Acids Res. 42,
D199D205 (2014). [pubmed] [pdf]
21) Kanehisa, M. and Goto, S.; KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res.
28, 27-30 (2000). [pubmed] [pdf]
22) BLAST Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schffer, Jinghui Zhang, Zheng
Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
23) Stephen F. Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro
A. Schffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted
substitution matrices", FEBS J. 272:5101-5109.

24) Jenkinson, C. P., Grody, W. W., & Cederbaum, S. D. (1996). Comparative properties of
arginases. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular
Biology, 114(1), 107-132.
25) Ash, D. E. (2004). Structure and function of arginases. The Journal of nutrition,134(10), 2760S2764S.
26) Momany, Cory, et al. "Crystallographic structure of a PLP-dependent ornithine decarboxylase
fromLactobacillus30a to 3.0 resolution." Journal of molecular biology 252.5 (1995): 643-655.
27) Chattopadhyay, M. K., C. W. Tabor, and H. Tabor. 2003. Polyamines protect Escherichia
coli cells from the toxic effect of oxygen. Proc. Natl. Acad. Sci. USA 100:2261-2265.

28) Li, X., & Coffino, P. (1992). Regulated degradation of ornithine decarboxylase requires interaction
with the polyamine-inducible protein antizyme. Molecular and cellular biology, 12(8), 3556-3562.

29) Jackson, L. K., Brooks, H. B., Osterman, A. L., Goldsmith, E. J., & Phillips, M. A. (2000). Altering
the reaction specificity of eukaryotic ornithine decarboxylase. Biochemistry, 39(37), 11247-11257.

30) Almrud, J. J., Oliveira, M. A., Kern, A. D., Grishin, N. V., Phillips, M. A., & Hackert, M. L. (2000).
Crystal structure of human ornithine decarboxylase at 2.1 resolution: structural insights to
antizyme binding. Journal of molecular biology, 295(1), 7-16.
31) Kern, A. D., Oliveira, M. A., Coffino, P., & Hackert, M. L. (1999). Structure of mammalian ornithine
decarboxylase at 1.6 resolution: stereochemical implications of PLP-dependent amino acid
decarboxylases. Structure, 7(5), 567-581.
32) McGinnis, M. W., Parker, Z. M., Walter, N. E., Rutkovsky, A. C., Cartaya-Marin, C., & Karatan, E.
(2009). Spermidine regulates Vibrio cholerae biofilm formation via transport and signaling
pathways. FEMS microbiology letters,299(2), 166-174.
33) Spivey, H. O., & Ovdi, J. (1999). Substrate channeling. Methods, 19(2), 306-321.
34) Miles, E. W., Rhee, S., & Davies, D. R. (1999). The molecular basis of substrate
channeling. Journal of Biological Chemistry, 274(18), 12193-12196.
35) Okar, D. A., Lange, A. J., Manzano, ., Navarro-Sabat, A., Riera, L., & Bartrons, R. (2001). PFK2/FBPase-2: maker and breaker of the essential biofactor fructose-2, 6-bisphosphate. Trends in
biochemical sciences, 26(1), 30-35.
36) Hajishengallis, G., Liang, S., Payne, M. A., Hashim, A., Jotwani, R., Eskan, M. A., ... & Curtis, M.
A. (2011). Low-abundance biofilm species orchestrates inflammatory periodontal disease through
the commensal microbiota and complement. Cell host & microbe, 10(5), 497-506.
37) Osterman, A. L., Brooks, H. B., Jackson, L., Abbott, J. J., & Phillips, M. A. (1999). Lysine-69 plays a
key role in catalysis by ornithine decarboxylase through acceleration of the Schiff base formation,
decarboxylation, and product release steps. Biochemistry, 38(36), 11814-11826.

38) Grishin, N. V., Osterman, A. L., Brooks, H. B., Phillips, M. A., & Goldsmith, E. J. (1999). X-ray
structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure
in complex with -difluoromethylornithine.Biochemistry, 38(46), 15174-15184.
39) Chaudhuri, B. N., Lange, S. C., Myers, R. S., Chittur, S. V., Davisson, V. J., & Smith, J. L. (2001).
Crystal structure of imidazole glycerol phosphate synthase: a tunnel through a (/) 8 barrel joins
two active sites. Structure, 9(10), 987-997.
40) Elcock, A. H., Potter, M. J., Matthews, D. A., Knighton, D. R., & McCammon, J. A. (1996).
Electrostatic channeling in the bifunctional enzyme dihydrofolate reductase-thymidylate
synthase. Journal of molecular biology, 262(3), 370-374.

41) Sigel, A. (2000). Manganese and its role in biological processes. New York: Marcel
Dekker. Print. Accessed via google books.