ZENIX Diagnostics
ZENIX Diagnostics, Briloner Landstrade,
Korbach -GERMANY
Rev1.2e
Note:
Indications, advices and suggestions. Italics are used to differ from each
other.
Upward
Dampproof
Fragile
Stack
Content
How to use this manual?...................................................................2
Signals used on the packing box......................................................3
Warning and Safety Indications........................................................4
1. Brief introduction of instrument....................................................8
1.1. Product introduction....................................................................................8
1.1.1
1.1.2
1.1.3
1.2.
1.3.
1.4.
1.5.
Product name............................................................................................8
Model........................................................................................................8
Product features........................................................................................8
Front view...............................................................................................13
Keypad....................................................................................................14
Back view................................................................................................15
Internal printer.........................................................................................15
Flow-cell and Cuvette.............................................................................15
Lamp and peristaltic pump......................................................................17
Principle structure figure (Only for reference).........................................19
2.
Operation.................................................................................20
2.1.
2.2.
2.3.
2.4.
2.5.
Power on...................................................................................................20
Function introduction of main menu.........................................................20
How to operate the software?..................................................................21
System settings........................................................................................21
Sample test...............................................................................................22
2.5.1.
2.5.2.
3.
Report......................................................................................35
4.
Power off..................................................................................40
5.
Maintenance............................................................................41
5.1. Overview...................................................................................................41
5.2. Clean instrument......................................................................................41
5.2.1.
5.2.2.
Change fuse............................................................................................42
Adjustment of peristaltic pump tube........................................................42
Change of aspiration tube.......................................................................42
1.
Product name
Semi-auto Chemistry Analyzer
1.1.2
Model
ZN-188
1.1.3
Product features
Flow-cell :
Flow-cell
Interface:
Display:
Print:
Work environment:
Storage environment:
Weight:
Overall dimensions:
7 kg
360mm(L)318mm(W)160mm(H)
Power supply:
Power input:
Fuse type:
Fuse specification:
AC110/220V10%, 50/60Hz1Hz
80VA
520
3.15A/250V
10
Open the instrument package, and take the materials used in transportation away.
Preserve the packing box and materials well, and its convenient for you to repack the
instrument someday in the future.
Take off the packing materials, and take the instrument out of the plastic package.
Check the articles inside the package box, and confirm the following should be
included:
Find a place without direct sunshine in your working place. The surface of the selected
working table should be flat, and there is enough space for placing ZN-188 Semi-auto
Chemistry Analyzer. The anterior edge of instrument should be near to the edge of
working table. Avoid comparatively large shake of working table (For example, put
hydroextractor on the working table).
11
1.5.3
1.5.4
AC110/220V10%
50/601 Hz
80VA
Warning:
Alternating current power must be well earthed (near-ground
voltage<5V).
Alternating current power must be stable. It is prohibited to use power
supply with high-power equipments together, and its better to equip
with voltage-regulated power supply.
In the area with unstable voltage, please use UPS power supply to
ensure the normal work of instrument.
When power line is pulled out, you must hold plug itself, not the
power line.
If there is smoke, abnormal smell or strange sound of instrument, cut
off the power at once, and contact with the distributor.
1.5.5
Install flow-cell
As the shown in the following figure 1-1, turn the light source cover open by hands.
12
13
Warning:
Please dont use hands to touch the surface of the light inlet window
of Flow-cell, so as to avoid leaving the lipid of hands on the light inlet
window and affecting the transmittancy.
Ensure the aspiration tube is in the socket, otherwise, it will cause
pressure and bend affecting the test effects. Its easy to result in
inspiriting bubbles, and water blank will be high.
1.6. Main structure
This part explains the structure of all the parts of ZN-188 Semi-auto Chemistry Analyzer.
14
1.6.2. Keypad
Figure 1- Keypad
Direction key: Cursor moves in the screen of four
directions, up, down, left and right.
Paper-feeding key: Feed paper by hand (It is also
the shortcut key for parameter printing)
Rinse key: Clean the pipeline.
15
6) Fuse
7) AC input
8) Fan
4) Drain port
9Backplate label
5) Power switch
16
Note:
While turning the light source cover open, first youd better
emphatically press the protuberant block of the two sides of the
cover, make both sides deform internally, and then open to the
cover according to the direction of following figure.
17
Thermostatic bath
Flow-cell
18
19
20
2.
Operation
2.1. Power on
Open the power switch on the back of the instrument, several seconds later, a menu as 2-1
will appear in the screen:
3 Sys. Setup
2 Report
4 Power Off
Ver1.0e
2006-11-21 16:20:08
Test: Edit the parameter settings, calibration settings and quality control settings of
the test item, it will save the parameters automatically after ensuring, and enter the
test process to perform item test.
2.
Report: Perform the data administration function of the test system. Its functions
include printing by test and patient numbers, deleting the test results, and
searching the quality control information of the current month.
3.
Sys. Setup: Set up the basic system operations. It includes printer settings, power
on and off, time settings, externally connecting administration system to transmit
all the test data of the current day.
4.
Power off: It is mainly used to wash the tubes after tests. Consumers can directly
cut off the power supply on the premise of ensuring the tubes are not needed to be
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1 Printer Setup
2 Time Setup
ID:600901001
3 Data Transfer
Figure 2-3 System settings menu
1. Print settings
In the menu of system settings, press key 1, the instrument will enter the following menu,
as figure 2-4.
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Transferring...
following three lines indicate item name and its corresponding serial number in orders. You
can use direction key, number key or name search to select items, the details is as
following:
123
Index 2
1 ABS
2 ALT
3 AST
4 LDH-L
5 ALP
6 LDH-P
7 GGT
8 CK-NAC
9 HBDH
10 AMS
11 ACP
12 CK-MB
1) Select by direction key: Select items by up, down, left and right keys, the selected
item will be highlighted, and its corresponding number will display at the top-left of
the screen.
2) Select by input: The input methods are displayed at the top-right of screen, they
are 123 and ABC respectively, and use . key to switch. 123 means number
input, numbers can be inputted directly at the moment, it will automatically select
the item according to the serial number, and display with highlight. ABC
manifests character input, input the characters according to the indication of
Keyboard, and it will display at the middle-top of screen. After finishing the input,
press ENTER key to automatically select the corresponding item (if there is no
corresponding item, there is no response).
2.5.1.2 Item parameters editing
Select the objective item, press ENTER key, and the parameter setting of corresponding
item will be performed, as figure 2-9 and 2-10.
Item Name : ALT
Method
Unit
: Kinetics
: umol/L
Delay Time: 30
Temperature: 37C
Test Time : 60
Wavelength
Decimal Digits : 3
: 340
Blank
: Reagent
Standard Setup :
Factor
: 1746.0
Control Setup :
24
2)
3)
4)
5)
6)
on the
method test, i.e. when absorbance value begins to change in linear, measure
the absorbance difference value of the two points in the linear part, and thus
concentration of reaction solution is obtained.
3) Unit: Select the test result unit, including 7 selectable units of mg/L, g/L, umol/L,
mmol/L, mol/L, U/L and IU/L, etc. Choose the unit by
key.
4) Temperature: Select test temperature, including 4 selectable temperatures of ambient
temperature, 25, 30 and 37, etc. Choose the temperature by
key.
5) Dominant wavelength: Choose the test dominant wavelength according to the kit
requirements, including 5 selectable wavelengths of 340nm, 405nm, 500 nm, 546 nm
and 570 nm. Choose the dominant wavelength by
key.
6) Sub wavelength: Select the test secondary wavelength according to the kit
requirements, and the system will flexibly implement dual wavelength method by
setting secondary wavelength. It includes 6 selectable secondary wavelengths of 340
nm, 405 nm, 500 nm, 546 nm, 570 nm and none. When single wavelength is used to
test, secondary wavelength must be set as none. Choose secondary wavelength by
key.
7) Blank: Select the blank testing, including none, reagent blank and sample blank.
Choose the blank type by
key. In the endpoint method test, reagent blank and
sample blank can be selected according to the consumer requirements. Two points
method test can choose reagent blank, and kinetics method has no blank settings.
8) Factor: Input the calculative factors. The system supports direct inputting factors and
determining calculative factors by system calibration (standard test).
9) Norms high: The inputted result is the value of norms high of the judgment evidence.
10) Norms low: The inputted result is the value of norms low of the judgment evidence.
11) Delay time: Input the delay time of the test process. The delay time is the time from the
tested fluid entering flow-cell to the start of actual test.
Note:
In order to make the test samples balance temperature and eliminate
tiny bubbles in the flow-cell, a certain time delay is necessary.
Most endpoint method tests, time delay is 5 seconds;
Kinetics method below 25, time delay should not less than 10
seconds;
Kinetics method below 30, time delay should not less than 12
seconds;
Kinetics method below 37, time delay is no less than 15 seconds.
12) Test time: Input the reaction time of the test procedure (endpoint method does not
need this parameter).
13) Decimal point: The reserved digit after the decimal point of result. The maximum is 4.
14) Aspirate volume: The aspirated solution amount of peristaltic pump. This parameter
26
indicates the amount of imbibed fluid to be tested each time. In order to ensure the
accuracy of test, aspirate volume is generally more than 400uL. (General items should
set as 500L; the aspirate volume of reagents with great pollution can be increased to
700L).
Note: Generally, the aspirate volume of 500uL can ensure the cross
contamination ratio below 1%. The aspirate volume can be
increased or reduced, and it is concretely regulated by the number
input on keyboard.
15) Standard setup: Set calibration parameters. Cursor points to Standard Setup, press
key to enter the setup menu of standard parameter according to the indications, as
figure 2-11.
Method: The calculation methods of standard test are linear regression and non-linear
regression respectively. Choose by
key.
Number of standards: The number of standard preparations used in the standard test
process. The maximum is 8 to calibrate.
Number of times of repeat: The repeat times of the test of each standard preparation.
Standard concentration: Standard preparation concentration used in the standard test
process.
Method
: Linear
Num of STD : 1
Num of Repeat: 1
Concentration : 133.00
Figure 2-11 Standard parameter setup menu
Note:
When non-linear regression is selected, the number of standard
should be more than 3, otherwise calibrate according to the linear
regression method;
When more than two standards are selected, the repeated number is
uniformly managed according to single time;
When more than three standard concentrations are inputted, it must
be inputted by progressive or degressive method; otherwise the
calibration is not successful. During the treating process of standard
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Method
: Linear
Num of STD : 3
Num of Repeat: 1
Concentration3: 133.00
<>Con.
2
Control No : 1
Average Value : 133.0
Batch No
SD: 2.0
: 060791
After finishing setting all the parameters and ensuring, press ENTER key to automatically
save parameter settings and enter item test process, thus it must be ensured to input the
correct parameters.. Pressing ESC key will turn back to the item menu without saving.
2.5.1.4 Item user-definition
The instrument can save 60 test items, including 47 fixing items and 13 user-defined items;
meanwhile, item user-definition also can be implemented directly on the basis of 47 fixing
items. The concrete user-defined methods are as following:
1) Move cursor to the Name column of parameter settings. At the moment, item name is
highlighted, or displayed as cursor symbol (no item name);
2) Directly input item name according to the character index figure on keyboard, and the
name includes 5 characters at the most. During the inputting process, it will
automatically ascertain characters at the interval of 3 seconds, and indicate inputting
next character;
3) During the inputting process, press DEL key to delete the inputted characters;
4) Set item parameters, press ENTER to automatically save the user-defined item name
and parameters, and enter test process.
Switching...
29
Please
Reag.
Blank
Redo
corresponding test process, and press ESC key to return to this test selection menu in the
corresponding test menu.
30
Standard
None!
Test
Control Test
Standard
Test
Test
Control
Sample Test
Sample Test
Sure
Test ALT
Index 1
Status
Con.
C=133.00
Feed Please
31
Test ALT
Index 1
Result
Con.
C=133.00
K=1746.078
b=0.012
Save?
Sample Test
Sample Test
the serial number of the current quality control solution, and the corresponding quality
control batch number is manifested on the right side. For example, Batch: 070691, please
ensure whether quality control preparations with same batch number are inputted before
aspirating quality control solution. Status displays the progress of test process and test
results. Feed please!, testing and save? are displayed one by one in the middledown area of menu. The detailed test procedure is the same with item test process; please
refer to the specific operation of item test.
Test ALT
Index 1
Batch 070691
Status
Feed Please
Figure 2-23 Quality control test menu
3) Test saving
After aspirating the corresponding quality control solution according to the steps, system
will calculate the results on real time. Quality control results are displayed, and quality
control conditions are judged in Result column (C>2SD means quality control result is
larger than the value of two SD, otherwise, this information will not be displayed), and it is
indicated save? in the system condition column. After ensuring quality control is effective,
press ENTER key to save quality control results. When double quality control is selected,
test the two quality control preparations in order. Aspirate corresponding quality control
solution according to the indication, and valid quality control results are saved (Only one
quality control result of the same item and same batch number is saved everyday), as
figure 2-24. Press ESC key to redo quality control. After quality control test, system will
automatically skip to the test selection menu and point to item test, as figure 2-18.
Test ALT
Index 1
Result
Batch
070691
C=75.078 C>2SD
Save
1) Test operation
When sample blank is selected during the parameter settings, sample blank is performed
first before sample test, as figure 2-25(a). When no blank or reagent blank is chosen, it has
already been finished during the former blank test, and it will directly enter test menu, as
figure 2-25(b).
Blank and Test will display the item name of current test.
>print on or >print off on the right upper corner of the window means to open and
close the online print function by
key, which refers to immediately print results after
finishing the test. It only can be changed on the condition of waiting for aspirating fluid.
Index displays the next sample number to be tested, it can be changed before the
test, and it will indicate Correct? by highlight on the right side of the corresponding
column. It is suggested to ensure whether the number is correct before the test,
otherwise, the former test result will be automatically overlapped by the same item and
same number.
Status displays the progress of test process, and Result indicates the test
absorbance and concentration.
Feed please!, Testing and Printing are displayed in order in the middle-down
part of the condition column, please inject the sample or wait for the test according to the
indications. When Overlapped? appears after testing, it is need to make sure whether to
overlap the same sample of the very day. ENTER is input directly will overlap, or a new
sample number is input before ENTER will save as the new sample.
Blank ALT
Index
10
Status
>Print On
Correct?
Feed Please
(a) Sample blank
>Print On
Test ALT
Index
10
Status
Correct?
Feed Please
2) Test process
Take no sample blank (select no blank or reagent blank) as an example, the procedure and
related operations are introduced in details. If sample blank is selected, sample blank will
be performed before each item test, and automatically enter sample test to wait for
aspirate the test sample. Its operation is completely the same with that of the sample test.
a
Confirm sample number (the displayed sample number is the number of the
sample to be tested), and open or close online print function by
key, as figure
2-25.
b
On the condition of Please Feed!, prepare the test sample, press aspiration key
to imbibe sample preparation. System will imbibe the fluid according to the set
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Test ALT
10
Index
Status
21
Testing...
Figure 2-26 Sample test condition
c
d
e
After finishing test, the test results are automatically saved (if this item already
has the test result of the same sample number on that very day, Overlapped?
will appear and wait for responding. ENTER will overlap, but a new number and
ENTER will save as the new sample). Meanwhile, the sample number will
increase one automatically as the number of next test sample, which is provided
to the consumers to revise.
Print the results. If online print function is selected, open or close by
key under
the condition of waiting for imbibing fluid.
When the current sample test and print are completed, the sample number will
increase one, and enter the condition of waiting for fluid aspiration of next sample
test.
Perform according to the indications, it can continuously test samples. When
quality control, calibration or exit is needed during the test procedure, press ESC
key to return to the test selection menu, and choose the related operations.
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3.
Report
Report is mainly in charge of the test results and the quality control test of the current
month. Meanwhile, perform the basic functions of data search, print and delete, etc.
In the main menu of system, press 2 key to enter administration menu, as figure 3-1.
They are sample report, item report and control report respectively, and the corresponding
function modules are entered by number key 1 , 2 and 3 .
Sample report: Search the test results according to the date and sample number;
Item report: Search the test results according to the date and item number;
Control report: Search the quality control results according to the month, item number and
batch number of quality control.
1 Sample Report
2 Item Report
3 Control Report
Note: ZN-188 can save 2200 test results at the most. System has data
renew ability by itself. When the test data exceeds the system
storage capacity, system will automatically delete parts of the old
data. At the same time, system will have the related indications
during the renewing procedure. Please wait for a moment, it can
automatically complete, and start test again.
3.1. Sample report
Input time range and sample number to search for the test results. Input start date and end
date respectively, meanwhile, the end date must behind the original date or the same day.
Input the sample number to be searched in Sample No. - means from A number to B
number, and B must not be less than A at the same time. When A equals to B, single
36
sample will be searched. As figure 3-2, it will search the test results of sample 1, 2 and 3
tested on November, 9th, 2006.
From
2006-11-09
To
2006-11-09
Sample No
1-3
Figure 3-2 Sample report search
After inputting time range and sample number, press ENTER key to search the results met
the conditions. If no related record is searched, None will be displayed on the right-down
of the window, as figure 3-3(a). If the related record is searched, all the condition satisfied
records will be displayed, as figure 3-3(b).
From
2006-11-09
To
2006-11-09
Sample No 1-3
(a) No record
None
In the display window of searched results, as Figure 3-3(b), several following modes of
shortcut key are defined:
Page up/down: A single page only can display 3 records, and the records of more pages
can be displayed by turning pages with
key.
switch number and character input mode. As Figure 3-4. It will search for all the results of
ALT item tested on November, 9th 2006.
From 2006-11-09
To
2006-11-09
Item No 2
ALT
From 2006-11-09
To
2006-11-09
Item No 2
ALT
None
3
(a) No record
ALT
132.098
In the display window of searched results, as Figure 3-5(b), several following modes of
shortcut key are defined:
Page up/down: A single page only can display 3 records, and the records of more pages
can be displayed by turning pages with
key.
control. Input test date, input the searched item number in item No, and display the item
name corresponding to the number on the right side at real time (input characters in
character mode switched by key . ). Input the required quality control batch number in
Batch No. As Figure 3-6. It will search for the quality control results of ALT item with the
batch number of 070691 on November, 2006.
Index
1
(a) No record
Result
Batch
132.098
070691
None
In the display window of searched results, as Figure 3-7(b), several following modes of
shortcut key are defined:
Page up/down: A single page only can display 3 records, and the records of more
pages can be displayed by turning pages with
key.
39
Test Result
Quality Control
Figure 3-8 Quality control search condition
Here will delete all the test results or quality control results. One record can be deleted
respectively as described at section 3.2 or section 3.3.
40
4.
Power off
After finishing all the tests, turn back to the main menu, press key 4 to perform the poweroff program. When the screen displays as Figure 4-1, press ENTER key (if press ESC key,
it will not be power off, and return to the main menu).
Power Off
Rinse
Feed Please
Rinsing...
After cleaning flow-cell, the system displays Cut off please!. As the display of Figure 4-4.
5.
Maintenance
5.1. Overview
ZN-188 Semi-auto Chemistry Analyzer is a clinical precise analyzer, and daily
maintenance work must be well done to keep the instrument in a good condition. The
maintenance of ZN-188 Semi-auto Chemistry Analyzer is very simple, but it must be
serious and careful.
Warning: Please dont let any solvent, lip and erosive material touch the
instrument.
5.2.2. Clean Flow-cell
Flow-cell must be kept clean, so as to ensure the accuracy and reliability of the test results.
1Clean the outside of flow-cell
a
Flow-cell must be placed according to the requirements. (refer to 1.5.5)
b
If the outside of flow-cell is polluted, soft cloth moistened with dehydrated alcohol
can be used to slightly clean.
2Clean the inside of flow-cell
c
Put the container with distilled water under the aspiration tube, press RINSE key,
and start the continuous washing function. Press RINSE key again, and washing
is finished. It is usually continuously washed for half a minute.
d
Glassware cleaner, or dilute hydrochloric acid (0.1N), Tween 20 diluents (2-3
42
drops/L) is used to clean flow flow-cell. Press RINSE key, aspirate cleaner, press
RINSE key again to stop the rotation of peristaltic pump, make cleaner stay in
flow-cell for 5 minutes, and finally use distilled water to continuously wash for 1
min. If it is not cleaned after washing once, cleaner can be used to wash again.
Flow-cell must be cleaned under the following conditions:
At the moment of power on, the different value of water blank is too big.
Change test items.
Before power off.
Warning: Dont leave the reaction fluid or other pollutants in the flow-cell
for a long time.
5.3. Instrument maintenance
5.3.1. Change fuse
Fuse is installed in the fuse box beside the power switch of the back of the instrument,
open the box cap, and its very convenient to change the fuse.
Fuse specification: 250V, 3.15A.
Users are suggested to use voltage regulated power supply.
Note: If the pump tube is not installed well, the instrument will not
aspirate.
43
Compass
tube
Aspiration
tube
Fixing tube
Trouble
1Analyzer can not be started.
Shooting
Check whether the instrument is on switch.
Check whether the power plug is loose.
Check the fuse.
Check the voltage.
2) The lamp of photometer is not lighted. Check power supply before changing lamps.
If lamp is damaged, change lamp.
3) Internal printer can not print.
running.
Check the connection of the interfaces of aspiration tube and
flow flow-cell, etc.
Aspiration tube is too long or too short.
Aspiration tube may be blocked, and it should be cleared and
dredged.
Flow Flow-cell is very dirty.
45
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