Epidemiology
Introduction
1
Department of Epidemiology and Public Health, Royal Free and University College London Medical School, London, UK; 2Diabetes Epidemiology Group,
Steno Diabetes Center, Gentofte, Denmark; 3School of Social Sciences, The Cathie Marsh Centre for Census and Survey Research, University of Manchester,
Manchester, UK. Correspondence:Alexandros Heraclides (axhr@steno.dk)
Received 13 August 2010; accepted 14 March 2011; published online 19 May 2011. doi:10.1038/oby.2011.95
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pants lost to follow-up were more likely to be women and to come from
the lower employment grades, were slightly older, had a slightly higher
BMI and had a higher prevalence of work stress at baseline.
The current analysis includes 5,138 white participants (3,689 men and
1,449 women) free from diabetes at baseline and with valid data on incident diabetes, psychosocial work stress and all the covariates used in the
multivariate analysis (sub-section following). Ethical approval for the
study was obtained from the Joint UCL/UCLH Committees on the Ethics
of Human Research. All participants gave written informed consent for
participation at each phase.
Assessment of glycemia and ascertainment of diabetes
At phase 3 (19911993), venous blood samples were taken from fasting
individuals (8h of fasting) before undergoing a standard 2-h oral glucose tolerance test (75g anhydrous glucose over 5min). Glucose samples were drawn into fluoride monovette tubes which were centrifuged
on site within 1h. Plasma or serum was immediately removed from
the monovette tubes, and moved into microtubes and stored at 70C.
Blood glucose was measured with the glucose oxidase method (15) on
YSI model 23A glucose analyzer (mean coefficient of variation at phase
3 2.93.3%) (16) and YSI model 2300 STAT PLUS analyzer (phases 5
and 7 mean coefficient of variation 1.43.1%) (17) (YSI Corporation,
Yellow Springs, OH). Subsequent clinical assessments for diabetes took
place at phases 5, 7, and 9. The definition of diabetes used was a 2-h
glucose tolerance test finding of at least 200mg/dl (11.1mmol/l) or
a fasting glucose level of 126mg/dl (7.0mmol/l) (18) or physiciandiagnosed diabetes and/or use of diabetic medication.
Psychosocial work stress
The Job Strain Questionnaire was developed to provide an integrating
theoretical framework for stress-related job characteristics that can be
assessed for the full workforce (19). In more detail, the questionnaire
assessed the aggregate of psychological stressors affecting work (job
demands) and the individuals potential control over job-related decision making (decision latitude).
In the Whitehall study, job demands (4 items; Cronbachs = 0.67) and
decision latitude (15 items; Cronbachs = 0.84) were measured using the
main questions from the Job Strain Questionnaire (19). The empirical
association between components of the Job Strain Questionnaire and
psychological strain has been previously demonstrated in relation to
depression, sleeping problems, and exhaustion (19).
According to the original demands/control model, high job demands
were identified as above the median score and low job control as below
the median score for the specific sample. Job strain was present when
the participant simultaneously scored high on the job demands (above
median score) and low on the decision latitude scales (below median
score) (19).
BMI and other covariates
Weight was measured by a Soehnle scale to the nearest 0.1kg with all
items of clothing removed except underwear. Height was measured to
the nearest mm using a stadiometer with the participant standing completely erect with the head in the Frankfort plane. BMI was calculated
as weight (kg) divided by height (m) squared. Obesity was classified
according to the World Health Organization definition (20).
Participants reported their Civil Service grade title, which was assigned
to 1 of 6 grades based on salary scale. In the British civil service employment grade is an accurate measure of status, income and employment
relations and hence socioeconomic position (21). Participants were asked
to report how much they were upset from personal illness, death or illness
of a close relative or friend, major financial difficulty, divorce, separation
or break of a personal intimate relationship, other marital or family problem, experience of a mugging, robbery, accident or similar event. From
these questions, a variable scored from 0 to 21 was developed measuring the extent by which participants were upset by life events outside
work during the recent past. Other social, psychosocial, and psychological variables (such as marital status, neighborhood deprivation, anger,
obesity | VOLUME 20 NUMBER 2 | february 2012
During an 18-year follow-up (19912009) and 114,447 personyears at risk, 927 new T2DM cases were identified. The 18-year
incidence of T2DM was 8.10 (95% confidence interval: 7.59;
8.64). Participants diagnosed with T2DM during follow-up
were older, more likely to be in the low employment grade,
were more upset by life events outside work and had a higher
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Table 1 Baseline (19911993) characteristics of the 5,138 participants included in analysis stratified by psychosocial work stress
Job strain (n = 1,387)
Agemean (s.e.)*
48.7 (0.08)
49.1 (0.35)
Women (%)**
34.2 (474)
26.6 (998)
15.2 (211)
13.4 (503)
39.1 (542)
28.6 (1,073)
3.13 (0.08)
2.74 (0.05)
34.7 (481)
32.2 (1,208)
12.4 (172)
9.0 (338)
15.6 (216)
12.3 (461)
3.41 (0.04)
3.58 (0.07)
25.5 (0.9)
25.5 (0.5)
85.6 (0.30)
86.7 (0.17)
119.6 (0.33)
121.0 (0.21)
1.46 (0.03)
1.48 (0.02)
1.42 (0.01)
1.42 (0.01)
1.88 (0.11)
1.87 (0.06)
Obese
1.00
Job strain
0.90
Survival probability
No job strain
No job strain
0.80
0.70
Job strain
0.60
0.50
0
10
12
14
16
18
20
10
12
14
16
18
20
Follow-up (years)
Figure 1 Kaplan-Meier curves showing cumulative survival probabilities for incident type 2 diabetes by baseline job strain during 18 years of followup among nonobese and obese participants (men + women) in the Whitehall II study.
BMI, systolic blood pressure, triglycerides and lower highdensity lipoprotein cholesterol (characteristics of the phase 3
Whitehall II sample stratified by follow-up diabetes status can
be found elsewhere) (12).
Table1 shows baseline characteristics of the 5,138 participants with data on all covariates included in analysis (540 incident T2DM cases) stratified by exposure to work stress (job
strain). The prevalence of job strain in this sample at analysis
baseline was 27% (25% in men and 32% in women). Compared
to participants with no job strain, those with baseline job
strain were slightly older, more likely to be women and to come
from a lower employment grade, had an unhealthier lifestyle
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Table 2Hazard ratios (95% confidence intervals (CIs)) for the association between job strain and incident type 2 diabetes by BMI
All men
Cases/total
389/3,689
Cases/total
151/1,449
Cases/total
HR (95% CI)
310/3,429
HR (95% CI)
P for interactiona
79/260
0.17
HR (95% CI)
Cases/total
HR (95% CI)
Cases/total
HR (95% CI)
P for interactiona
104/1,248
47/201
0.005
Adjusted for age, employment grade, diet pattern, alcohol consumption, physical activity, smoking status, systolic blood pressure, triglycerides, high-density lipoprotein
cholesterol. Comparing participants with and without job strain (no job strain is the reference category).
a
Interaction between job strain and BMI tested both with the continuous and binary (BMI <30kg/m2 vs. BMI 30kg/m2) variables with similar results (P values presented
are for the continuous variable).
Obese YES/
Stress YES
Obese YES/
Stress NO
Obese NO/
Stress YES
Obese NO/
Stress NO
Figure 2 Hazard ratios (95% confidence intervals (CIs)) for the effect
of job strain on 18-year incident type 2 diabetes after cross-classifying
participants by body weight status and exposure to work stress
among men.
Obese YES/
Stress YES
Obese YES/
Stress NO
Obese NO/
Stress YES
Obese NO/
Stress NO
Figure 3 Hazard ratios (95% confidence intervals (CIs)) for the effect
of job strain on 18-year incident type 2 diabetes after cross-classifying
participants by body weight status and exposure to work stress
among women.
with a lower risk of T2DM in the nonobese (HR0.70: 95% confidence intervals 0.53; 0.93) but not in the obese (PINTERACTION
= 0.17). In contrast, among women, job strain was associated
with higher risk of type 2 diabetes in the obese (HR 2.01: 1.06;
3.92) but not in the nonobese (PINTERACTION = 0.005). This analysis was repeated stratifying by overweight and obesity (BMI
25kg/m2 vs. BMI <25kg/m2) as well as for central obesity
(waist circumference >102cm for men and >88cm for women;
Adult Treatment Panel III definition). In both cases very similar results to those reported in Table2 were obtained.
Figures 2 and 3 show HRs for incident T2DM cross-classifying participants by work stress (job strain) and obesity (BMI
30kg/m2) in men and women, respectively. Participants not
exposed to work stress and who were not obese served as the
reference category in this analysis. As expected, among both
men and women, the obese had a higher risk of T2DM compared to the nonobese. Among men, the nonobese stressed had
lower risk of T2DM than the nonobese nonstressed (Figure2).
Among women, the obese stressed had higher risk of T2DM
than the than the obese nonstressed (Figure3). The risk associated with co-occurrence of obesity and work stress among
women was over and above the risk associated with the two
exposures individually.
Discussion
Summary of findings
In this sample of middle-aged British civil servants, the association between work stress and incident T2DM was modified
by BMI differentially among men and women. Work stress
was associated with a lower risk of T2DM among nonobese
men, while it was associated with a higher risk of T2DM
among obese women. There was no evidence for an association between work stress and T2DM among obese men and
nonobese women.
The major strength of the current analysis is the accurate
assessment of all key factors (psychosocial stress, obesity and
diabetes). The Whitehall II study was specifically designed to
assess the impact of psychosocial factors on chronic disease
and thus assessment of psychosocial work stress is detailed and
comprehensive. Diabetes was ascertained by an oral glucose
tolerance test at consecutive phases, which is rare for a population-based epidemiological study. Obesity was also accurately
assessed using weight and height measured by trained nurses.
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In addition, the prospective design and long follow-up of the
study allows for a detailed investigation of long-term diabetes
risk using a large number of incident cases.
Some weaknesses of the current analysis are the self-reported
nature of the exposure of interest (psychosocial work stress)
and the fact that only a single assessment at one point of time
was used here. However, despite being based on subjective
data, the job strain measure has been linked to indicators of
psychological strain such as depression, sleeping problems,
and exhaustion (12). Another weakness is the substantial loss
to follow-up, which differed by baseline exposure to work
stress and was more apparent for obese women. If however
this was a potential source of bias it would have led to a type
II error (failing to find an existent association) rather than
a type I error (finding an association that dos not exist). In
the current results, the magnitude of the effect of work stress
among women was relatively big and it may have been even
bigger if the obese, stressed women who were lost to follow-up
remained in the study.
Conclusion
candidate for explaining the stressobesity interaction in relation to T2DM is the stress-hormone cortisol (26). Cortisol can
interfere in the normal regulation of blood glucose by altering
the bodys release and sensitivity to insulin, thus increasing the
risk of T2DM (1,2). In a recent publication from the Whitehall
study (27), the slope of the diurnal release of cortisol was shallower among obese individuals, highlighting a possible defect
in the functioning of the hypothalamic-pituitary-adrenal axis
of the stress response. Given that cortisol levels are elevated by
exposure to psychosocial stressors (28), a plausible explanation
for the observed effect is that stress-related elevations in cortisol
levels carry a bigger pathophysiological burden among obese
than nonobese individuals.
The observation that obesity modifies the effect of work stress
on T2DM only among women could be explained by genderspecific pathways involved in pathogenesis of T2DM. In the
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Acknowledgments
The Whitehall II study was supported by grants from the Medical Research
Council; Economic and Social Research Council; British Heart Foundation;
Health and Safety Executive; Department of Health; National Heart Lung
and Blood Institute (HL36310), US, NIH; National Institute on Aging
(AG13196), US, NIH; Agency for Health Care Policy Research (HS06516);
and the John D and Catherine T MacArthur Foundation Research Networks
on Successful Midlife Development and Socioeconomic Status and Health.
We thank all participating civil service departments and their welfare,
personnel, and establishment officers; the Occupational Health and Safety
Agency; the Council of Civil Service Unions; all participating civil servants in
the Whitehall II study; and all members of the Whitehall II study team.
Disclosure
The authors declared no conflict of interest.
2011 The Obesity Society
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