LEB 01
12150.00
Power supply, 12 V
12151.99
14602.00
14504.61
Neurosimulator
65963.00
65963.93
07361.02
P4010511
LEB
01.05
Neurosimulator:
Membrane time constant and low-pass filtering
Related topics
Time constant of nerve membrane, intracellular potential,
low-pass characteristic
Principle and task
To use a nerve function model to work on the following
themes:
The time constant of the nerve membrane and intracellular potential
The low-pass characteristic of the nerve membrane
Equipment
Cobra3 Basic Unit
Power supply, 12 V
RS232 data cable
Cobra3 Universal Recorder software
Neurosimulator
Neurosimulator, power supply
Connecting cord, 500 mm, yellow
PC, Windows
12150.00
12151.99
14602.00
14504.61
65963.00
65963.93
07361.02
Set-up
Connect the instruments as shown in Fig. 1.
1
1
1
1
1
1
1
Phywe Series of publication Laboratory Experiments Biology PHYWE SYSTEME GMBH & Co. KG D-37070 Gttingen
40105
21
LEB
01.05
Neurosimulator:
Membrane time constant and low-pass filtering
Experiment 2:
Click on the red point and go to measurement with
CONTINUE
Tip the switch of stimulation channel 1 repeatedly and
at increasingly shorter intervals; save the result
Results and evaluation
Experiment 1: When the neuro-simulator is excited by
a rectangular pulse from a synapse, the intracellular
potential only increases slowly, and then falls off even
slower when the stimulation stops (Fig. 3). As a result
of this behaviour, rapid and brief stimulating signals
can only be weakly transmitted (low-pass characteristic of the membrane)
Experiment 2: When short rectangular impulses act at
relatively large intervals, the intracellular potential can
follow the stimulating pulse frequency (Fig. 4). The
low- pass characteristic of the membrane allows the
conversion of the low frequencies to a response.
When the stimulating frequency is higher, the intracellular potential reaches an almost constant value.
Individual rectangular stimulations then no longer
cause a separate response of the membrane.
Note
Many further experiments can be carried out in a similar way using 1, 2, 3 or 4 neurosimulators (see the
Operating Instructions).
22
40105
Phywe Series of publication Laboratory Experiments Biology PHYWE SYSTEME GMBH & Co. KG D-37070 Gttingen