com
a
Department of Veterinary Medicine Federal University of Lavras, Lavras, Brazil
Science Veterinary University, National University of Rosrio (FCV-UNR), Santa Fe, Argentina
c
Department of Animal Science, Federal University of Lavras, Lavras, Brazil
Received 3 May 2011; received in revised form 11 November 2011; accepted 22 November 2011
Abstract
The objective was to evaluate the influence of the timing of hormonal induction, using gonadorelin or common carp pituitary extract
(CPE), on the reproductive activity of female Astyanax bimaculatus. Fish (N 44) were weighed, measured, and acclimatized to
experimental conditions with a photoperiod of 12 h:12 h light:dark (L:D) for 10 days. Ovulation was induced with a single dose of CPE
(6 mg/kg) or gonadorelin (80 g/kg), given at 12:00 (halfway through the light phase (LP) or 24:00 (halfway through the dark phase
(DP), in a 2 2 factorial design. The time of ovulation was calculated in degree hours and daily motor activity was recorded using a
photocell. The fish were killed and the liver and gonads were weighed for calculation of gonadosomatic (GSI) and hepatosomatic (HSI)
indexes, respectively. Absolute fecundity (AF), absolute fecundity relative to weight (AFRW) and length (AFRL), diameter of oocytes
(mM), and percentage of oocytes with the germinal vesicle in a peripheral position (PPGV) were recorded. All females responded
(ovulated). The female Astyanax bimaculatus had twilight motor activity rhythm. Females given CPE at 12:00 had a higher (P 0.05)
percentage of oocytes with the germinal vesicle in a peripheral position compared with the group that received gonadorelin in the same
period (95 6 vs. 79 21%, mean SD). The absolute fecundity relative to weight was higher in groups induced at 12:00, regardless
of the hormone used (LP: 805 448 and 700 214, for CPE and gonadorelin, respectively; dark phase: 580 396 and 529 105,
P 0.05). Both times used for hormonal induction with CPE and gonadorelin were suitable for inducing reproduction in lambari,
although induction with CPE in LP had the best results.
2012 Elsevier Inc. All rights reserved.
Keywords: CPE; Daily rhythm; Gonadorelin; Oocytes; Induction of ovulation; Fish
1. Introduction
Daily biological rhythms are associated with environmental fluctuations that have a periodicity of 24 h
(circadian rhythm), entrained to the cycle of light and
1571
1572
Diameter
(m)*
PPGV (%)*
DHO*
AF
AFRL
GSI (%)
HSI (%)
AFRW
Period of
application
Hormone
Carp pituitary
extract
Gonadorelin
LP
1066.5 142.2
989.2 78.6
DP
LP
DP
LP
DP
LP
DP
LP
DP
LP
DP
LP
DP
LP
DP
1135.4 49.1a
95 6a
86 26
333.9 30a,A
240.1 13B
7443 6501
5241 4660
818 625
594 484
18.0 7.9
14.0 9.3
1.0 0.6
1.2 1.0
752.5 330.0A
554.5 250.0B
932.7 98.8b
79 21b
93 8
250.3 14b
240.3 11
5290 1769
4119 1170
636 201
486 119
14.2 3.3
10.6 2.4
1.9 1.6
1.3 0.9
1573
1574
Acknowledgments
Funding was provided by FAPEMIG, CAPES, and
CNPQ. Tortuga Zootcnica Land Co. provided the gonadorelin.
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