Roll No.

Total No. of Question: 40

Time: 2:00 Hrs.

Total No. of Pages: 03

M.Sc. I-ZOOLOGY EXAMINATION

ANALYTICAL AND MOLECULAR TECHNIQUES &
MICROSCOPY
SUBJECT CODE: ZOM104

INSTRUCTION TO CONDIDATES:
1. All Questions are compulsory and have FOURTY questions of one mark each.

Max.

1. Which instrument would be used to

examine living cells that are attached to
other surfaces, such as artery plaques?
a. Phase-contrast microscope
b. Scanning acoustic microscope
c. Fluorescence microscope
d. Confocal microscope
2. For viewing which of the following would a
scanning electron microscope not be the
instrument of choice?
a. The complexity of T-even bacteriophages
b. The helical structure of deoxyribonucleic
acid (DNA)
c. Human erythrocytes (red blood cells)
d. E. coli bacteria
3. Which of the following describes the
correct path of light in a compound light
microscope, from the illumination source to
the eye of the observer?
a. Condenser lenses prism specimen
objective lenses body tube ocular lens
eye
b. Ocular lens body tube condenser
lens specimen objective lens prism
eye
c. Objective lenses specimen
condenser lenses body tube prism
ocular lens eye
d. Condenser lenses specimen
objective lenses body tube prism
ocular lens eye
4. Which of the following is a lens found on
electron microscopes but not on light
microscopes?
a. Objective lens b Projector lens
c. Condenser lens d. Eyepiece lens
5. What is the fate of the electrons that
interact with a specimen in an electron
microscope?
a. They are reflected by the specimen.
b. They are refracted by the specimen.
c. They may be absorbed, reflected, or
refracted by the specimen.
d. They are absorbed by the specimen

6. In which type of microscopy do the

specimens appear to be three-dimensional
(3-D), allowing their external features to be
viewed best?
a. Scanning electron microscopy (SEM)
b. Transmission electron microscopy (TEM)
c. Phase-contrast microscopy
d. Darkfield microscopy
7. In the decolorizing step of the Gram stain,
which reagent is used?
a. Alcohol-acetone b. Malachite green
c. Iodine
d. Crystal violet
8. Regarding the Gram stain technique, which
of the following is the primary stain?
a. Crystal violet
b. Safranin
c. Iodine
d. Carbolfuchsin
9. You find colorless areas in cells in a Gramstained smear. What should you do next?
a. A capsule stain b. A simple stain
c. A flagella stain d. An endospore stain
10. Which microscope is used to see internal
structures of cells in a natural state?
a. Phase-contrast microscope
b. Compound light microscope
c. Electron microscope
d. Fluorescence microscope
11. Which microscope can be
visualize DNA or botulinum toxin?
a. Compound light microscope
b. Scanning tunneling microscope
c. Scanning electron microscope
d. Phase-contrast microscope

used

to

12. Cells are differentiated after which step in

the Gram stain?
a. Alcohol-acetone b. Iodine
c. Crystal violet
d. Safranin
13. Which of the following microscopes uses
visible light?
a. Scanning electron microscope
b. Confocal microscope
c. Scanning acoustic microscope
d. DIC

d. Fluorescence microscope
14. Which microscope is used
intracellular detail in a living cell?
a. Two-photon microscope
b. Transmission electron microscope
c. Brightfield microscope
d. Fluorescence microscope

to

see

15. The resolution of a microscope can be

improved by changing the
a. Fine adjustment. b. Wavelength of light.
c. Coarse adjustment.
d. Condenser.
16. Molar absorbtivities of compounds
exhibiting charge transfer absorption are
a. small
b. moderate
c. large
d. none of these
17. Which of the following is not an IR
vibrational mode?
a. Stretching
b. Scissoring
c. Rocking
d. Rolling
18. In the equation, A = bc, what quantity is
represented by ""?
a. absorbtivity
b.
molar
absorbtivity
c. path length
d. none of these
19. Where does a carbonyl (C=0) stretch
appear in an IR spectrum?
a. 1740-1720
b. 1870-1650
c. 3640-3250
d. 160-110
20. Which of the following components of a
monochromator is the dispersing element?
a. The collimating lens
b. The entrance
slit
c. The diffraction grating d. None of these
21. Vibrational spectroscopy is
a. a large mass on a weak spring
b. a flashlight through a prism and shake it
c. a class of spectroscopic techniques which
analyzes molecular motions
d. an Infrared spectroscopy
22. Which microscope is used to see detail of
a 300-nm virus?
a. DIC microscope
b. Phase-contrast microscope
c. Electron microscope

23. The appearance of gram-negative

bacteria after addition of the decolorizing
agent in the Gram stain.
a. Purple
b. Red
c. Colorless
d. Brown
24. Thin layer chromatography is
a. partition chromatography
b. electrical mobility of ionic species
c. adsorption chromatography
d. none of the above
25. In reverse phase chromatography, the
stationary phase is made
a. non-polar
b. polar
c. either non-polar or polar
d. none of
these
26. Ion exchange chromatography is based
on the
a. Electrostatic attraction
b. Electrical mobility of ionic species
c. Adsorption chromatography
d. Partition chromatography
27. Which microscope uses two beams of
light to produce a three-dimensional, color
image?
a. Darkfield microscope
b. Electron microscope
c. Phase-contrast microscope
d. DIC microscope
28.
Van
Leeuwenhoek's
microscope
magnified up to 300x. This was a(n)
a. Compound microscope.
b. Electron microscope.
c. Simple microscope.
d. Confocal microscope.
29. A combination of paper chromatography
and electrophoresis involves
a. Partition chromatography
b. Electrical mobility of the ionic species
c. Both (a) and (b)
d. None of these
30. Which microscope achieves the highest
magnification and greatest resolution?
a. Compound light microscope

b. Phase-contrast microscope
c. Darkfield microscope
d. Electron microscope
31. What is used to cool the superconducting
coil?
a. Hydrogen
b. Ice
c. Dry ice
d. Liquid helium

c. An acid dye.

d. A mordant.

40. In reversed phase HPLC, there is a

a. Non polar solvent/polar column
b. Polar solvent/non-polar column
c. Non polar solvent/non-polar column
d. Any of the above

32. The purpose of the ocular lens is to

a. Magnify the image from the objective lens.
b. Decrease the light.
c. Improve resolution.
d. Increase the light.
33. HPLC stands for
a . High Pressure Liquid Chromatography
b. High Performance Liquid Chromatography
c. both (a) and (b)
d. Highly Placed Liquid Chromatography
34. The eluent strength is a measure of
a. Solvent adsorption energy
b. Solvent absorption energy
c. Solvent diffusivity
d. Solvent mixing index
35. HPLC methods include
a. Liquid/liquid (partition) chromatography
b. Liquid/solid (adsorption) chromatography
c. Ion exchange and size exclusion
chromatography
d. All of the above
36. For a typical adsorbent such as silica gel,
the most popular pore diameters are
a. 10 and 50 A
b. 60 and 100 A
c. 100 and 150 A d. 150 and 200 A
37. What Gram reaction do you expect from
acid-fast bacteria?
a. Gram-negative b. Gram-positive
c. Both (a) & (b)
d. Can't tell
38. Which microscope is used to see
intracellular detail in a living cell?
a. Two-photon microscope
b. Transmission electron microscope
c. Brightfield microscope
d. Fluorescence microscope
39. The counter stain in the acid-fast stain is
a. A basic dye.
b. A negative stain.

ANSWER KEY

1-b
6-a
11-b
16-c
21-c
26-a
31-d

2-b
7-a
12-a
17-d
22-c
27-d
32-a

3-d
8-a
13-d
18-b
23-c
28-c
33-c

4-b
9-d
14-a
19-b
24-c
29-c
34-a

5-c
10-a
15-b
20-c
25-a
30-d
35-d

36-b

37-b

38-a

39-a

40-b