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SAMPLE IN CLASS ASSIGNMENT

Name:__________________

Date:___________________

1. What are the 2 bacteria normally found on the teeth?


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2. The aforementioned bacteria will break down sucrose into its components. What are the
2 components of sucrose (the monosaccharides)?
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3. The bacteria referred to in question 1 will break down the 2 monosaccharide components
of sucrose into bacterially generated byproducts. (a) What are these byproducts, and
(b) from which specific monosaccharide are they generated from?
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4. Referring to the byproducts in question 3, how does each product contribute to dental
carries?
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5. A lab technician streaks 10uL of urine onto a MAC plate. After doing the appropriate
calculation (CFU/mL) the technician establishes that there were 1,000 Colony Forming
Units per mL of urine.
a. Why would the technician use the MAC (MacConkey Agar) for this urinalysis?
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b. Does this person have an infection? Why do you think that (what is the critical
number of colonies for MAC plate)?
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SAMPLE IN CLASS EXAMINATION QUESTIONS

1. In order to kill the bacterial in a nutrient broth, a student decided to place the culture into
boiling water.
a. Water usually boils at __________________ C.
b. The student then streaked the boiled culture onto a nutrient agar, and was surprised to
find a small amount of bacterial growth. How could this have happened?
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2. DNA is prone to damage once exposed to UV radiation. With that said, UV radiation is
classified into 3 categories depending on the frequency of the light rays (A, B, and C)
a. Which UV (A, B, or C) would damage DNA?
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b. A specific type of DNA damage occurs when nucleotides form a dimer. What specific
nucleotide forms this dimer?
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3. The visible light spectrum (red-violet) is generally between (Circle Best Answer):
a. 200nm and 400nm
b. 400nm and 600nm
c. 400nm and 800nm
d. 800nm and 1,000nm
e. 600nm and 1,000nm

4. Below is a graph plotting the rate of growth (y axis, vertical) against temperature (x axis,
horizontal). Along the graph is the optimal growth of 5 different types of bacteria
(labeled A, B, C, D, and E), classified according to their temperature preferences. Use
this graph to answer the following questions:
a. Which bacteria (A, B, C, D, or E) would grow best at room temperature?

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b. Which bacteria (A, B, C, D, or E) would grow best in a boiling hot springs? _______
c. What is the name for bacteria listed as C? _________________________________
d. What is the name for bacteria listed as D? __________________________________

E
C
B
A

5. (Fill in the blanks) There are 2 general classes of antimicrobial agents used to control
bacterial growth. ___________________________________ are used on inanimate
objects, such as operating room tables, whereas _______________________________
are used on living tissue, such as the skin.

6. The disk diffusion method was used to test the effectiveness of various antibiotics against
S. aureus (pictured below) Of the 4 antibiotics tested, which was the most effective? Why
did you come to this conclusion? Please use proper terminology when referring to
observed results.
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