Lecturer, 2Prof and Head, Dept of Chemistry,, Sri Siddhartha Institute of Technology, Tumkur, Karnataka, India
3
Head R&D, Clov Chem. India Private Limited, Harohalli, Bangalore. Karnataka, India.
4
Asst.Prof. Department of Chemistry, Govt First Grade College and PG Centre, Davanagere, Karnataka, India
Abstract
A copolymer of N-vinylpyrrolidone (NVP) with maleic anhydride (MA) is synthesized by radical copolymerization with 2,2-Azobisisobutyronitrile(AIBN) as initiator at 650C in dioxane solution, under nitrogen atmosphere. The copolymer obtained is
characterized by FTIR and NMR. The copolymer is made to interact with N,N-Diethylaminoethanol (DEAE) in different ratios. The
susceptibility of some Gram - negative bacteria like Klebsiella aerogenes
NCIM-2098, Escherichia coli NCIM-5051,
Pseudomonasaeruginosa NCIM2242, Pseudomonas desmolyticum NCIM-2028 and a Gram positive bacteria Staphylococcus aureus
NCIM-5022, to the copolymer and its compound with N,N-Diethylaminoethanol of different compositions in microbiological medium
is studied by Agar well diffusion method. The resulting polymeric antibacterial material is useful in a number of medicines and food
industry.
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2. EXPERIMENTAL
2.1 Materials
2.1.1 Chemicals
N-vinyl-2-pyrrolidone (NVP) (Sigma-Aldrich) was purified
before use by distillation under moderate vacuum. Maleic
anhydride (MA) (Lobachem) was purified before use by
recrystallisation from anhydrous benzene solution and
sublimation in vacuum. 2,2-Azobisisobutyronitrile (AIBN)
was purified by successive crystallization from chloroformmethanol mixture. N,N-Diethylaminoethanol was purified by
distillation. The solvents used as copolymerization medium,
for precipitation and extraction were all of analytical grade.
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Fig 1: Reaction scheme of N-Vinylpyrrolidone and Maleic anhydride, hydrolyzed copolymer and poly(NVP-Co-MA) / DEEA macro
complex
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1441.30cm-1 C-N-C,
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I
II
III
IV
Standard
(5g/50L)
M
(100g/100L)
M1
(1000g/100L)
M2
(1000g/100L)
Klebsiella
aerogenes(B1)
(MeanSE)
12.670.33**
Escherichia
coli (B2)
(MeanSE)
13.670.33**
Pseudomonas
aeruginosa(B3)
(MeanSE)
13.670.33**
Staphyloccus
aureus (B4)
(MeanSE)
13.670.33**
Pseudomonas
desmolyticum(B5)
(MeanSE)
14.670.33**
1.330.33**
1.330.33**
2.670.33**
inactive
2.670.33**
2.670.33**
2.000.00
3.670.33**
inactive
3.000.00
1.670.33**
2.670.33**
5.670.33**
inactive
1.000.00
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60
Fig 5: Representative photograph showing Antibacterial activity in Agar well diffusion method
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4. CONCLUSIONS
The Copolymer (NVP MA) was synthesized. Its structure
was confirmed by FTIR, H1_ NMR and C13_ NMR. It was
made to react with DEEA after hydrolysis. The anti bacterial
activity of Copolymer and its macro complex was determined
by agar well diffusion method. It was demonstrated that the
Copolymer and its macro complex have a positive effect in
controlling the growth of the Gram ve bacteria. Gram +ve
Bacteria were found to be active in the presence of these
compounds. This fact can be explained by the different
responsive behavior of surface layer structures of these two
bacteria. The activity of M2 is more when compared to M and
M1. This may be due to the combination of the functional
groups within the single molecule. This new polymeric
biocide system can be recommended for the biomedical and
food industry application.
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62
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63
BIOGRAPHIES
Hemalatha P, Obtained her PG degree in
1997 securing IV rank and a gold medal in
Physical
Chemistry
at
Bangalore
University. Served as lecturer in First Grade
College and PU Colleges at Bangalore and
Tumkur. At present working as lecturer at SSIT and pursuing
Ph.D. in VTU, Belgaum, Karnataka, India.
Dr. M K Veeraiah, He was awarded
Doctorate in 1998 from the University of
Mysore. Two of his students are awarded.
He is guiding three research scholars. His
areas of interests are Chemical kinetics,
Polymers and corrosion studies. He has a
credit to his fame in publishing 20 papers both in International
and National journals. He has 39 years of experience in
teaching and 22 years of experience in research and
development.
DR. S Prasanna Kumar, He has been
conferred Doctoral in 2005 from
Kuvempu University. He has 15 years of
Experience in Polymer Synthesis,
Coatings & Adhesives. He has rendered
many number of projects and headed the
R & D sections in leading polymer
companies. He has published 16 papers in national and
international journals.
Anasuya K V, Obtained her PG Degree
from Bangalore University in 1997 with
II rank and two gold medals for securing
highest marks in special subject
Inorganic Chemistry. She has 5 years of
experience and 15 years experience of
teaching chemistry in PU and degree colleges. Presently she is
pursuing her Ph.D. in VTU, Belgaum, Karnataka, India.
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