KEY WORDS:
Angiogenesis inhibitors.
Retinal neovascularization.
Corneal angiogenesis.
Macular degeneration.
Review.
ABSTRACT
CONTEXT AND OBJECTIVE: Many eye diseases involve increased local levels of vascular endothelial growth factor (VEGF), and there are several therapeutic
strategies for them. Thus, the aim of this study was to evaluate the effectiveness and safety of bevacizumab for treating eye diseases involving increased
local levels of VEGF, as the assumed pathophysiological mechanism.
DATA SOURCES: The following databases were systematically searched for evidence: PubMed, CENTRAL (Cochrane Library), Literatura Latino-Americana
e do Caribe em Cincias da Sade (Lilacs) and reference lists, without language restrictions. Only randomized controlled trials were included. The primary
outcome of interest was visual acuity, irrespective of the evaluation method.
DATA SYNTHESIS: A total of 667 eyes in nine randomized trials were included. Meta-analysis showed that the proportion of patients with age-related
macular degeneration who presented improvements from baseline regarding best-corrected visual acuity was higher among those treated with bevacizumab
than among those in the photodynamic therapy group (risk ratio, RR, 0.49; 95% confidence interval, CI, 0.31 to 0.78; P = 0.01).
CONCLUSIONS: The evidence available demonstrates that bevacizumab alone or combined with other treatments is more effective than other options,
including photodynamic therapy, focal photocoagulation and triamcinolone. The use of bevacizumab instead of photodynamic therapy could reduce
treatment costs by more than 99% and could significantly increase access to treatment. However, long-term studies are still needed in order to reduce
uncertainty concerning the safety of this medication for all ocular neovascular diseases in which bevacizumab has the potential to improve visual acuity.
RESUMO
PALAVRAS-CHAVE:
Inibidores da angiognese.
Neovascularizao retiniana.
Neovascularizao da crnea.
Degenerao macular.
Reviso.
CONTEXTO E OBJETIVOS: Muitas doenas oculares envolvem o aumento dos nveis locais de fator de crescimento do endotlio vascular (FCEV), uma
diversidade de estratgias teraputicas para tais condies. Assim, o objetivo do presente estudo avaliar a efetividade e a segurana de bevacizumabe
para o tratamento de pacientes com doena ocular que envolva o aumento dos nveis locais de FCEV, como mecanismo patofisiolgico assumido.
FONTE DAS INFORMAES: Foi realizada busca sistemtica pelas evidncias disponveis nas seguintes bases de dados da eletrnicas: PubMed,
CENTRAL (The Cochrane Library), Literatura Latino-Americana e do Caribe em Cincias da Sade (Lilacs), alm de referncias bibliogrficas de estudos
relevantes, sem restries de lngua. Foram includos apenas ensaios controlados e aleatrios. Acuidade visual, independentemente do mtodo de
avaliao, foi considerada o desfecho primrio de interesse.
SNTESE DOS DADOS: Foi includo um total de 667 olhos testados em nove ensaios clnicos aleatrios. A metanlise demonstrou que a proporo de
pacientes com degenerao macular relacionada idade que melhoraram a acuidade visual foi maior entre os tratados com bevacizumabe do que entre
os pacientes em terapia fotodinmica (risco relativo [RR] 0.49, 95% intervalo de confiana [IC] 0,31 a 0,78, P = 0,01).
CONCLUSES: A evidncia disponvel demonstra que bevacizumabe isolado ou combinado com outras terapias mais eficaz que terapia fotodinmica,
fotocoagulao focal e triancinolona. O uso de bevacizumabe em vez da terapia fotodinmica poderia reduzir os custos do tratamento em mais de 99% e
aumentar significativamente o acesso ao tratamento. Entretanto, o aspecto de segurana do frmaco ainda necessita ser avaliado por estudos em longo
prazo com todas as doenas neovasculares em que bevacizumabe tenha o potencial de melhorar acuidade visual.
MSc. Affiliated researcher at Brazilian Cochrane Center and postgraduate student at the Discipline of Emergency Medicine and Evidence-Based Medicine, Department of Medicine,
Universidade Federal de So Paulo Escola Paulista de Medicina (Unifesp-EPM), So Paulo, Brazil.
2
MD, PhD. Full professor of the Discipline of Ophthalmology, Department of Ophthalmology, Universidade Federal de So Paulo Escola Paulista de Medicina (Unifesp-EPM), So
Paulo, Brazil.
3
MD, PhD. Full professor of the Discipline of Emergency Medicine and Evidence-Based Medicine, Department of Medicine, Universidade Federal de So Paulo Escola Paulista de
Medicina (Unifesp-EPM), So Paulo, Brazil.
1
84
INTRODUCTION
METHODS
Data sources and searches
The following databases were searched: Medical Literature Analysis and Retrieval System Online (Medline) (1966 to June 2008), Controlled Trials Register of the Cochrane Collaboration (2008, issue 2)
and Literatura Latino-Americana e do Caribe em Cincias da Sade
(Lilacs) (1982 to June 2008). A general search strategy was used, with
adaptations to the characteristics of each database, to identify studies on
bevacizumab for ocular diseases that involved increased local levels of
VEGF. Descriptors and synonyms for the intervention of interest (Avastin, bevacizumab) and for the clinical conditions for which this medication potentially could be indicated were used (e.g. age-related macular
disease, corneal neovascularization, retinal angiomatous proliferation or
angiogenic retinal diseases, among others).
Study selection
We planned to include only randomized or quasi-randomized clinical trials that tested bevacizumab alone or in association with other
strategies available. The clinical condition of interest among the individuals included (of both genders, independent of ethnicity and age) was a
diagnosis of ocular diseases or ocular conditions with the same underlying pathophysiological mechanism of increased local levels of VEGF, according to the criteria established by the authors of the primary studies,
such as age-related macular disease, corneal neovascularization, retinal
angiomatous proliferation or angiogenic retinal diseases, among others.
100
237 studies
50
237 studies
0
2 3 3 3 3 3 3 4 4 4 4 4 4 5 5 5 5 5 5 6 6 6 6 6 6 7 7 7 7 7 7 8 8 8
c-0 b-0 pr-0 n-0 g-0 ct-0 c-0 b-0 pr-0 n-0 g-0 ct-0 c-0 b-0 pr-0 n-0 g-0 ct-0 c-0 b-0 pr-0 n-0 g-0 ct-0 c-0 b-0 pr-0 n-0 g-0 ct-0 c-0 b-0 pr-0 n-0
De Fe A Ju Au O De Fe A Ju Au O De Fe A Ju Au O De Fe A Ju Au O De Fe A Ju Au O De Fe A Ju
The chart of cumulative relative frequency demonstrates that 50% of the studies on the application of
bevacizumab for eye diseases were published from December 2002 to September 2007, according to
their indexation in PubMed. The remaining 50% (237 studies) was published over a period of only nine
months, from September 2007 to June 2008.
85
RESULTS
Description of studies with potential for inclusion
Out of the 474 studies retrieved through the search strategy, only
nine satisfied the preestablished inclusion criteria (Figure 2). A Cochrane systematic review covering all anti-VEGF therapies for age-related macular degeneration was found, but the authors had not included
studies testing bevacizumab by the time the present systematic review
was finished.13 One randomized study was excluded because its outcomes did not align with the purpose of this review and because it allocated the two eyes of each patient to different comparison groups.14
The nine studies included in the present review yielded a total of 667
randomized eyes, as shown in Table 1.15-23
Three studies included patients with diagnoses of diabetic macular edema.17,20,21 One study included patients with diagnoses of clinically significant macular edema who had not responded to earlier subsequent photocoagulation therapy.15 Three studies included patients with
Table 1. Comparison groups and bevacizumab regimen tested in each study
Reference
Intervention
15
a. bevacizumab
b. bevacizumab
triamcinolone
c. sham
16
17
18
19
20
21
22
23
86
Performance bias
With the exception of three studies15,17,21 that made use of control
groups receiving sham injections or laser, none of the other studies reported any care taken to prevent performance bias.
All studies retrieved using the search
strategy, across all databases (n = 474)
Studies excluded: narrative reviews, in vitro/in situ
studies, animal and diagnostic studies, case reports,
retrospective case series and studies not dealing
with the clinical condition or intervention
of interest (n = 383)
Prospective studies on humans that
dealt with the clinical conditions and
intervention of interest (n = 91)
Detection bias
The majority of the studies reported that the evaluators were unaware of the groups to which each patient (eyes) was allocated.15,16,19,21,22
Thus, only these five studies were considered to present a low risk of
detection bias. All the others were considered to present a high risk of
systematic error.
Attrition bias
None of the studies reported any substantial losses from their samples, either overall or among their comparison groups. For this reason,
all the studies were considered to present low risk of attrition bias.
Outcome measurements
Best-corrected visual acuity
Bevacizumab alone was shown to be better than the association of
bevacizumab and with triamcinolone for best-corrected visual acuity
(logMAR, change from baseline), but without a statistically significant
mean difference (MD) (MD, 0.02; 95% CI, - 0.09 to 0.14; P = 0.70].
However, in one study,15 the estimate effect favored the group treated
with bevacizumab in association with triamcinolone (MD, -0.03; 95%
CI, -0.13 to 0.07) (Figure 3, comparison 1; two studies15,21). Comparisons between bevacizumab (both in association with triamcinolone and
alone) and sham injections demonstrated statistically significant MD in
favor of the bevacizumab groups (MD, - 0.18; 95% CI, - 0.28 to -0.08;
P = 0.0003 and MD, - 0.15; 95% CI, - 0.26 to - 0.04; P = 0.008, respectively) (Figure 3, comparisons 2 and 3; one study15).
A statistically nonsignificant MD was observed for best-corrected
visual acuity (logMAR, endpoint), slightly favoring the group treated
with bevacizumab over the group treated with triamcinolone (MD,
0.01; 95% CI, - 0.04 to 0.06; P = 0.68) (Figure 3, comparison 4; one
study20). On the other hand, panretinal photocoagulation alone was
better, but without statistical significance, than when combined with
1.5 mg bevacizumab (MD, 0.02; 95% CI, - 0.12 to 0.16; P = 0.78)
(Figure 3, comparison 5; one study22). Bevacizumab (1.25 mg) in association with triamcinolone was also shown to be better than laser
photocoagulation alone (MD, - 0.11; 95% CI, - 0.30 to 0.08; P =
0.25) (Figure 3, comparison 6; one study21). For these two comparisons, the MD between the groups were not statistically significant.
On the other hand, comparison between bevacizumab alone and triamcinolone in association with photodynamic therapy showed a statistically significant difference in favor of bevacizumab with regard to
the endpoint of best-corrected visual acuity (P < 0.005) in one study.
However, the available estimated effect was not appropriate for inclusion in a forest plot.18
Bevacizumab alone was shown to be better than photodynamic
therapy for best-corrected visual acuity (logMAR, change from baseline), with a statistically significant MD (MD, - 0.09; 95% CI, - 0.13
to - 0.06; P < 0.00001) (Figure 3, comparison 7; one study19). Bevacizumab in association with photodynamic therapy was shown to be
better than both bevacizumab alone and photodynamic therapy alone,
with statistically significant MD (MD, -0.14; 95% CI, - 0.18 to - 0.11;
P < 0.00001 and MD, - 0.24; 95% CI, - 0.27 to - 0.20; P < 0.00001,
respectively) (Figure 3, comparisons 8 and 9; one study19).
Adverse events
Although the adverse events potentially associated with bevacizumab were of diverse types,24 the most common adverse events associated
with bevacizumab, irrespective of whether alone or not, were: moderate
anterior chamber reaction (19%),15 transient anterior chamber reaction
(16%),21 iris neovascularization (11%),15 subconjunctival hemorrhage,22
posterior vitreous detachment (15%)19 and foreign body sensation.22
More details about other adverse events are shown in Table 2.
87
Comparison
Left Treatment versus Right Treatment
Mean Difference
Study
Mean
SD
Total
Mean
SD
Total Weight IV, Random, 95% CI
1. Bevacizumab + triamcinolone versus bevacizumab alone (change from baseline)
Ahmadieh 2007
-0.21
0.19
37
-0.18
0.26
41 53.7% -0.03 [-0.13, 0.07]
Soheilian 2007
-0.13
0.31
33
-0.22
0.23
37 46.3%
0.09 [-0.04, 0.22]
Subtotal (95% IC)
70
78 100.0%
0.02 [-0.09, 0.14]
Heterogeneity: Tau2 = 0.00; Chi2 = 2.07, df = 1 (P = 0.15); I2 = 52%
Test for effect: Z = 0.38 (P = 0.70)
2. Bevacizumab + triamcinolone versus sham (change from baseline)
Ahmadieh 2007
-0.21
0.19
37
-0.03
0.24
Test for effect: Z = 3.58 (P = 0.0003)
Mean Difference
IV, Random, 95% CI
Figure 3. Mean best-corrected visual acuity (logMAR: endpoint and change from baseline).
DISCUSSION
Based on the primary outcome that we planned to analyze in this systematic review, the results showed that intraocular use of bevacizumab
alone was better than photodynamic therapy (alone or combined with
triamcinolone) for patients with subfoveal choroidal neovascularization
associated with age-related macular degeneration, choroidal neovascularization due to age-related macular degeneration and age-related macular
degeneration alone, as reported by the authors of the primary studies. Bevacizumab combined with photodynamic therapy was shown to be better than photodynamic therapy alone and bevacizumab alone for patients
88
Comparison
Left Treatment versus Right Treament
Outcome
Study
Events
Total
Events
Total
Weight
1. Bevacizumab versus photodynamic therapy (alone or combined with triamcinolone)
a. Patients who lost their visual acuity from baseline (irrespective of authors definition)
Bashushur 2007
0
32
8
30
38.7%
Weigert 2007
2
14
7
14
61.3%
Subtotal (95% CI)
46
44
100.0%
Total events
2
15
Heterogeneity: Tau2 = 0.31; Chi2 = 1.24, df = 1 (P = 0.26); I2 = 20%
Test for overall effect: Z = 2.15 (P = 0.03)
b. Best-corrected visual acuity > 3 lines
Bashshur 2007
32
32
Test for effect: Z = 2.71 (P = 0.007)
22
30
30
55
14
99
Risk Ratio
Risk Ratio
47.0%
13.7%
39.4%
100.0%
32
95% CI = 95% confidence interval; I2 = inconsistency or heterogeneity test, showing statistically significant (or substantial) heterogeneity between estimated effects from studies included, when I2 > 50%. Tau2 = variance
between studies in meta-analysis based on random effects. Intersection between horizontal lines (confidence intervals) and horizontal line (null hypothesis) means absence of statistically significant differences between
comparison groups. Gray lines illustrate the estimate effects (risk ratio between comparison groups). Lozenges illustrate resultant meta-analysis.
participants in the subgroup at high risk of proliferative diabetic retinopathy in both eyes was not random in one study.22 Furthermore, in
one study,23 the nature of the interventions allowed concealment of the
allocation and blinding of patients and therapists regarding the use of
bevacizumab or triamcinolone, but blinding would not be operationally
easy for photodynamic therapy. It was decided to include these studies,
given the lack of high-quality controlled studies on the application of
bevacizumab for treating ocular diseases at the time when this systematic review was implemented.
The fact that Mirshahi et al.14 was not included in this review deserves attention. Today, the internal validity of studies in which the eyes
of a single patient are allocated to different groups is considered to be
unclear. This is illustrated by the existence of contradictory studies. Indeed, there is evidence from studying the pharmacokinetic aspects of
bevacizumab that the possibility of contralateral effects (through systemic absorption of the drug by the contralateral eye) is remote.26 On
the other hand, there is evidence demonstrating the opposite, through
manifestations of adverse events associated with bevacizumab in the
89
Adverse events
Anemia17
Cataract progression19
Congestive heart failure17
Death17
Elevation of blood pressure17
Endophthalmitis17
Foreign Body sensation22
Intraocular pressure rise15,17,20,21,23
Iris neovascularization15
Marked anterior chamber reaction15
Moderate anterior chamber reaction15
Myocardial infarction17
Peripheral vascular disease17
Pigment epithelial tears19
Posterior vitreous detachment19
Progression of fibrous proliferation15
Subconjunctival hemorrhage22
Syncope17
Transient anterior chamber reaction21
Transient intraocular pressure rise17
Vitreous hemorrhage15
Worsened renal function17
Bv
% (n/N)
4 (4/90)
7 (4/54)
1 (1/90)
2 (2/90)*
1 (1/90)
1 (1/90)
4 (1/124)
22 (9/41)
2 (1/41)
19 (8/41)
2 (2/90)
1 (1/90)
3 (3/54)
15 (8/54)
2 (1/41)
1 (1/90)
19 (7/37)
1 (1/90)
0 (0/41)
3 (3/90)
Pg + Bv
% (n/N)
13 (2/15)
47 (7/15)
-
Tr + Bv
% (n/N)
8 (6/70)
0 (0/37)
3 (1/37)
19 (7/37)
0 (0/37)
12 (4/33)
3 (1/37)
-
PDT + Bv
% (n/N)
6 (3/50)
0 (0/50)
8 (4/50)
-
Comparison groups
Sham
Pg
% (n/N)
% (n/N)
5 (1/19)
0 (0/19)
0 (0/19)
0 (1/19)
0 (0/19)
0 (0/7)
0 (0/37)
0 (0/52)
0 (0/37)
0 (0/37)
0 (0/37)
0 (0/19)
10 (2/19)
0 (0/37)
0 (0/7)
0 (0/19)
0 (0/33)
0 (0/19)
0 (0/37)
0 (0/19)
PDT
% (n/N)
0 (0/52)
0 (0/52)
0 (0/52)
-
Tr
% (n/N)
7 (1/13)
-
PDT + Tr
% (n/N)
14 (2/14)
-
The percentages were obtained from all studies reporting at least one event in the bevacizumab group (irrespective of whether combined with other options or alone). Bv = Bevacizumab; Pg = Photocoagulation; Tr = Triamcinolone;
PDT = Photodynamic therapy.
*Reported causes of death were myocardial infarction and pancreatic cancer.
contralateral eye.7 Therefore, it seems sensible for the time being for
researchers to choose types of allocation other than involving different
treatments for each eye of the same individual.
The present scenario is that, taken together, the studies that have
been published are still of an exploratory nature, given the diversity of
comparisons, co-interventions, dosages and variables of interest (outcome measurements), along with the variety of ways of reporting these
variables. Thus, it is recommended that the specialists within this field
should come to a consensus regarding which outcomes are relevant and
how these should be analyzed.
It is possible to estimate the costs associated with bevacizumab.
Bashur et al. found that an average of 2.4 treatments with 2.5 mg bevacizumab per patient was needed to treat persistent subretinal fluid or cystic macular disease, and that 2.3 treatments with photodynamic therapy
were needed when leakage from choroidal neovascularization was present
on fluorescein angiography over a six-month period.16 The gross cost of
bevacizumab is about USD 5.5 per mg (United States dollars).27 The assumed cost of photodynamic therapy with verteporfrin, or Visudyne is
USD 3,000.00.28 The meta-analysis demonstrated that, in order to improve visual acuity in one patient, 3.45 patients (29/100) would have to
be treated with Bevacizumab and 8.25 patients (12/99) with photodynamic therapy (Figure 4, comparison 1c). The estimated expense would
be USD 113.85 for bevacizumab and USD 56,925.00 for photodynamic
therapy. Considering the findings of Brown et al.,29 an average of 11 treatments of 0.5 mg ranibizumab per patient would have to be administered,
at a cost of USD 3,900.00 per mg. 27 To improve visual acuity, defined as
15 letters at 12 months, 2.48 patients (56/139) would have to be treated with ranibizumab. The estimated cost would be USD 53,196.00.
90
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Sources of funding: Science and Technology Department of the Science Secretariat, Brazilian Ministry of Health. The sponsor had no role in the design and conduct of the study or
in collection, management, analysis and interpretation of the data or in preparation, review
or approval of the manuscript
Conflict of interest: None known
Date of first submission: October 24, 2008
Last received: March 31, 2009
Accepted: April 23, 2009
91