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By

Alfa- Joy V. Fallurin, Neil Julius Magsino, David Trinidad


BS BIOLOGY
Mrs. Celeste R. Desingano

Transport
through Cell
Membranes

INTRODUCTION
Cells make use of both passive and active processes to transport substances across their
membranes. Passive transport processes are those that require no metabolic energy from
the cell but rely solely on the physical properties of the substances themselves. Active
transport processes require energy expenditure by the cell to move substances whose
physical prevent their independent motion. In this exercise, we will observe large-scale
examples of passive transport processes.

OBJECTIVES:
To determine the difference in the diffusion of solid in cold and warm water.
To know the effects of osmosis (hypotonic and hypertonic) through the use of chicken
egg.
To understand more about filtration and its outcome.

MATERIALS AND EQUIPMENT

Potassium permanganate crystals


Petri dish
Boiling water (100 C) and ice water (0 C)
Forceps
Chicken egg ( soaked in vinegar in three days)
Syrup
Food coloring
Container
Distilled water
Filter paper
Glass funnel
Erlenmeyer flask
Copper sulfate
Boiled starch solution
Charcoal (powdered)

PROCEDURES
A.

Physical Basis of Passive Transport

1.

2.

Using forceps, place a small crystal of potassium permanganate


in the center of a petri dish of calm, hot water. Place a second crystal
of the same size in a dish of calm, cold water. Be careful not to
disturb the dishes by touching them or causing them to shake.
Observe the two dishes over a period of 30 minutes, carefully
noting any changes in the crystals.

B. Diffusion and Osmosis


1. Place a chicken egg (soaked in vinegar for three days) in a container
filled with food color and water, while the other egg in a container
filled with syrup.
2. Then wait and observe the egg for one week.
C. Filtration
1. Place a cone made of filter paper inside a glass funnel.
2. Position the funnel over an empty flask
3. Mix together 3ml of copper sulfate solution, 4ml of boiled
starch solution, a pinch of charcoal powder, and 5 to 8ml of
distilled water. Note the physical characteristics of each as
you mix them.
4. Pour the mixture
into the funnel.
5. Observe the mixture being
filtered by the paper.

RESULTS AND DISCUSSION


A.
1.

Physical Basis of Passive Transport

In cold water, the size of the diameter of the solid Potassium


permanganate is 85mm
and the circle is light violet in color.
2. In hot water, the size of
the diameter of the solid
Potassium
permanganate is 90mm
and the circle is dark
violet in color.

Conclusion
Therefore, potassium permanganate in hot water has a bigger
diameter than in cold water since hot water can easily dissolved the
Potassium permanganate.
B. Diffusion and Osmosis
1. The chicken egg, is placed in a container with food coloring and
water. It increases its size.
2. The chicken egg, is placed in a container with syrup, it decreases
its size.
Conclusion
Therefore, chicken egg in a container with food coloring and
water increases its size since
there is less solute and more
water resulting to a swell
and burst cell whereas in
chicken egg placed in a container
with syrup decreases its size
since there is more solute
and less water resulting to a
shrink and shrivel cell.

C. Filtration
1. The process become progressively slower and water is the
outcome of
the substance being filtered
( charcoal, cooper sulfate and
starch)
Conclusion
Therefore, the process
become progressively
slower since gravity
creates pressure needed to push the
substances in order for
water to across a paper
membrane.