2.2
Other Supplies
1 pair of exam gloves
2 pair of sterile surgical gloves
3 alcohol prep pads
2 betadine prep swabs
Ophthalmic antibiotic solution
Balance saline solution
2 sterile eye drapes
2 #15 blades
2 cotton balls
2 eye caps
1 pen light
1 10cc syringe
1 16 gauge needle
1 purple top venojet tube
2 red top venojet tube
1 gray top venojet tube
1 biohazardous bag
1 consent form
3.5.2
3.5.3
3.5.4
3.5.5
If any of the above signs are observed on the physical assessment and are
deemed to be an indication of either high-risk behavior or an indication of
HIV or hepatitis infection then the tissue should be rejected.
The procurement technician will perform a pen light exam on the donor
and document any findings on the Physical Assessment Form
The donors weight shall be documented on IV Infusion/Transfusion
Worksheet
Document the date and time the donor was refrigerated if applicable on the
Donor History Form
NOTE: If a donor is 12 years of age or under and there is no pre-transfusion sample, then an
algorithm should be applied when any transfusion or infusion has occurred to determine that
there has not been plasma dilution sufficient to affect test results.
3.6.2
4.6 Remove prep gloves and dispose of them in a biohazardous waste bag.
5. Prepare Sterile Field
1.1 Sterile Field
1.1.1 Prepare the sterile field by first placing the sterile instrument tray on your
prepared work surface.
1.1.2 Remove the plastic dust cover.
1.1.3 Before opening he sterile kit, verify that the instrument kit is sterile by
checking the expiration date and integrity of the wrap.
1.1.4 Carefully open the outer and inner wraps.
1.1.5 Open additional sterile supplies onto the sterile field such as blades, eye
drapes, cotton-tip applicators, and corneal chambers.
1.2 Sterile Sleeves & Gloves
1.2.1 Open outside packages of sterile sleeves, gloves, and towels.
1.2.2 Scrub utilizing the Brush-Stroke Method.
1.2.2.1 Wet hands and arms.
1.2.2.2 Wash hands and arms to 2 inches above the elbows with an antiseptic
agent.
1.2.2.3 With hands held under running water, clean under fingernails with
disposable nail cleaner; discard after use.
1.2.2.4 Rinse hands thoroughly under running water, keeping hands up and
allowing water to drip from elbows.
1.2.2.5 Take sterile brush/sponge impregnated with antiseptic from package.
1.2.2.6 Scrub nail of on hand 30 strokes; each side of each finger, starting with the
thumb, 20 strokes; the back of the hand 20 strokes; the palm of the hand
20 strokes; the arm 20 strokes for each 3rd and each side to 2 inches above
the elbow.
1.2.2.7 Repeat steps for the other hand and arm.
1.2.2.8 Rinse hands and arms thoroughly.
1.2.3 Drying
1.2.3.1 Reach down and lift towel from package, being careful not to drip water
on the package. Bend forward slightly to avoid letting the towel touch
your attire.
1.2.3.2 Dry both hands independently. Dry one arm holding towel in opposite
hand and using an oscillation motion of the arm.
1.2.3.3 Draw the towel up to the elbow
1.2.3.4 Carefully reverse the towel, holding it away from the body.
1.2.3.5 Dry the opposite arm on the unused, now uppermost end of the towel.
1.2.4 Donning Sterile Gloves and Sterile Sleeves
1.2.4.1 Gloving using Open Glove Technique without a gown. This method of
gloving skin-to-skin, glove-to-glove technique. . The hand, although
scrubbed, is not sterile and must not contact the exterior of the sterile
gloves. The everted cuff on the gloves exposes the inner surface. The first
glove is put on with skin-to-skin technique, bare hand to inside cuff. The
sterile fingers of that gloved hand then may touch the sterile exterior of the
second glove, that is, glove-to-glove technique.
1.2.4.2 With the left hand, grasp the cuff of the right glove on the fold. Pick up
the glove and step back from the table.
1.2.4.3 Insert the right hand into the glove and pull it on, leaving the cuff turned
well down over the hand.
1.2.4.4 Slip the fingers of the gloved right hand under the everted cuff of the left
glove. Pick up the glove and step back.
1.2.4.5 Insert the hand into the left glove and pull it on, leaving the cuff turned
down over the hand.
1.2.4.6 With the fingers of the right hand, pull the cuff of the left glove up and
avoid touching the bare wrist.
1.2.4.7 Repeat step 5 for the right cuff, using the left hand, and thereby
completely gloving the right hand.
1.2.5 Donning sterile sleeves.
1.2.5.1 Reach down to the sterile package and slide gloved hand under folded
upper portion of sleeve. Step back from the table and let the sleeve
unfold.
1.2.5.2 Being careful to keep gloved hand protected by the fold in the sleeve, slide
opposite hand into sleeve and pull through the cuff.
1.2.5.3 Pull the sleeve to wrist level only.
1.2.5.4 Do not push glove all the way through the sleeve.
1.2.5.5 Pull upper portion of sleeve to 2 inches above elbow. Again, being careful
to protect sterile, gloved hand with folded upper cuff.
1.2.5.6 Once you have pulled sleeve to above elbow, do not return to that area
with the gloved hand again.
1.2.5.7 Repeat, using opposite hand and sleeve.
1.2.6
Don the second pair of sterile gloves using open glove technique as described
above.
6. Enucleation
1.1 Check the instruments to be sure none are missing.
1.2 Apply sterile drapes to donor lids
1.3 Using cotton-tip applicator, gently open the upper lid by pulling towards the top of
the head; insert the closed lid speculum under the upper and lower lids. Take care
not to abrade the cornea with the speculum.
1.4 Grasp the conjunctiva with the forceps near the limbus. Cut and dissect the
conjunctiva using the small curved scissors 360 around the cornea, using blunt
dissection.
1.5 Using a muscle hook locate rectus muscle. Clamp the lateral rectus muscle with the
small curved hemostat. Locate and sever the remaining five muscles. After the five
muscles have been severed, cut the lateral rectus muscle distally to the hemostat.
1.6 Insert the closed blades of the large enucleation scissors behind the globe. Open the
blades and position the optic nerve between the blades. Push the scissors toward the
back of the orbit and cut the optic nerve.
1.7 Using the hemostat that is attached to the lateral rectus muscle, gently lift the globe
and excise any connective tissue.
1.8 Place the globe in the bed of cotton in the appropriate eye jar with the cornea facing
up.
1.9 Pour a small amount of balanced saline solution or antibiotic solution in the jar.
1.10 Remove the top layer of gloves and repeat steps 6.1 through 6.7.
7. Concluding steps
7.1 Remove drapes
7.2 Leave head elevated
7.3 Place a cotton ball in each empty orbit. Place an eye cap over the cotton ball and pull
the upper and lower lids shut.
7.4 Place the lids on both the eye jars and label each jar. Ocular tissue is put in a Ziploc
bag and placed on wet ice for transport. Upon return to the eye bank ocular tissue
shall be placed in the section marked Quarantined of the Jewett Refrigerator.
7.5 Clean off any remaining debris (i.e., betadine, blood) using balanced saline solution
and gauze.
7.6 Complete a toe tag and affix to the body and rewrap the donor body in the body bag
or shroud and return it to the storage area from which it was removed.
7.7 Follow steps described in Exposure Control (Section 10) below.
7.8 Call the hospital contact person and the funeral home to inform them that the
procedure is completed and the body may be picked up.
8. Documentation
1.1 Document the date and time of enucleation and the media used on the Donor History
Form.
1.2 Complete an Eye Enucleation Post Operative Form if the enucleation was performed
in the hospital. Leave one copy with the hospital and return the other to the eye bank
to be retained in the donor file.
9. Autopsy
1.1 If an autopsy is to be performed by the Medical Examiner the technician will draw a
vitreous sample after the enucleation procedure and prior to the excision. Place the
vitreous sample in the gray top tube provided in the kit. The tube shall be labeled
with the donors name, date & time the sample was drawn. The tube should then be
taped to the donors chest.
1.2 The technician will then fill out the Eye Evaluation Form for Coroner/Medical
Examiners and leave it with the body.
1.3 Document on the Donor History From the date & time and where the autopsy will be
performed.
10. Exposure Control
10.1 Discard all sharps in the appropriate Biohazardous sharps container.
10.2 Dispose of all personal protective clothing and equipment in appropriate
Biohazardous waste containers.
10.3 Wash hands thoroughly before leaving the work area.
11. References
11.1 EBAA Medical Standards; November 1998
11.2 EBAA Procedures Manual; October 1996, (E1.000 & E1.100)
12. Effective Date March 1, 1999
13. Approvals
13.1______________________________________________________________
Director of Technical Services
Date
13.2______________________________________________________________
Executive Director
Date
13.3______________________________________________________________
Medical Director
Date