Bacteriological Examination and Antibiogram of Milk Sample of Clinically Infected Dairy Cow Suffering From Mastitis (PDF Download Available)

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Bacteriological Examination and

Antibiogram of Milk Sample of
Clinically Infected Dairy Cow Suffering
from Mastitis
Article (PDF Available)January 2015 with 11 Reads
1st Praveen KUMAR Praveen
5.87 Arawali Veterinary College, ...
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Available from: Praveen KUMAR Praveen, Jan 15, 2016
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https://www.researchgate.net/publication/290447834_Bacteriological_Examination_and_Antibiogram_of_Milk_Sample_of_Clinically_Infected_Dairy_Cow_Suffe...
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Original Research
Bacteriological Examination and Antibiogram of Milk Sample of

Clinically Infected Dairy Cow Suffering from Mastitis
Subha Ganguly1,*, Arpita Padhy 2, Saraswat Sahoo3, Shyam Lal Garg4, Praveen Kumar Praveen5,
Rajesh Wakchaure6
1
Associate Professor & Head, 2Assistant Professor, Department of Veterinary Microbiology, 3Assistant Professor, Department
of Veterinary Gynaecology & Obstetrics, 4Teaching Associate, Department of Livestock Production Management, 5Assistant
Professor, Department of Veterinary Public Health & Epidemiology, 6Associate Professor, Department of Animal
Genetics & Breeding, Arawali Veterinary College, Rajasthan
*Corresponding Author:
Email: ganguly38@gmail.com
ABSTRACT
Mastitis is chronic inammation of the mammary gland of cattle and can have infectious and non-infectious etiology. It is
characterized by physical, chemical and usually bacteriological changes in the milk and pathological changes in the glandular
tissue of the udder and aects quality and quantity of milk. Mastitis is usually caused by bacteria that invade the udder, multiply
and produce toxins which are harmful to the mammary gland. It remains the most economically important disease of dairy
industries around the world producing great economic loss to farmers. There are two forms of mastitis viz., clinical mastitis
(CM) and Sub clinical mastitis (SCM).
Key Words: Antibiogram, Bacteriological examination, Mastitis, Milk
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9 References
specication of the manufacturer required for

INTRODUCTION
The indiscriminate uses of antibiotics and irrational
laboratory purpose. Incubation of the petridishes
treatment of bovine mastitis with dierent antibiotics
layered with the agar containing antibiotic discs was
done at 37oC for 24 h in a B.O.D. incubator installed at
have invited serious complications like multiple drug
resistance. Till date dierent types of antibiotics have
the department.
been tried against the pathogens in bovine mastitis with
or without identication and drug sensitivity testing
RESULTS AND DISCUSSION
(Patnaik et al., 2013; Paul et al., 2013; Ganguly, 2013).
The milk sample was subjected to spread plate culture
on Nutrient agar media plates. After incubation at 37oC
The present study was conducted to identify the
etiology of clinical mastitis and the antibiotics/
for 24h it revealed the presence of smooth, raised,
antibacterial drugs which show sensitivity against the
mucoid, circular colonies with regular edges. Grams
various pathogenic agents.
method of staining revealed Gram positive cocci shaped
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organisms arranged in the form of chains when
examined under the high power magnication of the
MATERIALS AND METHODS
Article: [Qualitative
of the resultsmicroscope.
of a method proposed
Milk sample was collected by hand stripping
method inevaluation
compound
The for bacteria
were
standardization
of
the
bacteriologic...
a sterile sample collection tube from the aected
bacteriologically determined to be grouped under
quarter of the udder of a cross bred cattle
exhibiting
Streptococcus spp. (Cruickshank et al., 1975; Buxton
Jul 1977
clinical symptoms of mastitis maintained at the
and Fraser, 1977; Fine gold and Martin, 1982;
Instructional Livestock Farm Complex (I.L.F.C.) of
Ananthanarayan and Paniker, 2009).
Arawali Veterinary College. The aected cattle were
Antibiotic assay revealed the bacterial isolates to be
clinically examined at the Teaching Veterinary Clinical
highly sensitive toShare
the antibiotics,
Ampicillin and
Complex (T.V.C.C.) of the college. The collected milk
Chloramphenicol with low degree of sensitivity to
sample was then produced to the Department of
Amoxicillin. The degree of sensitivity was determined
Veterinary Microbiology during November, 2015 for
on the basis of zone of inhibition formed by the isolated
bacteriological examination and reporting.
bacteria after exposure to the particular antibiotics.
The milk sample was examined bacteriologically
The results obtained on cultural properties of the
(Buxton and Fraser, 1977) by gar plate culturing and by
bacteria and its antibiotic disc diusion assay revealed
staining by Grams Method followed by antibiotic
in the present study was in correlation with the ndings
sensitivity test by Kirby-Bauer antibiotic disc diusion
of Kumar et al. (2010), Patnaiket al. (2014) and Paul et
assay method on Mueller-Hinton agar with certain
al. (2013).
modications (Patnaik et al., 2014) using antibiotic
CONCLUSION
discs provided by the supplier (Titan Biotech Ltd.,
Bhiwadi, Rajasthan, India). The concentration of

The present study revealed the presence
of
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Bhiwadi, Rajasthan, India). The concentration of

antibiotic in each lter paper disc was as per the
The present study revealed the presence of

Streptococcus spp. of bacteria responsible for causing
International Journal of Medical Microbiology and Tropical Diseases, October-December, 2015;1(1):6-7

Article: [Qualitative evaluation of the results of a method proposed for
standardization of the bacteriologic...
Jul 1977
See all
9 References
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Subha Ganguly et al.
Bacteriological Examination and Antibiogram of Milk Sample of Clinically.
clinical mastitis in dairy cattle. The bacterial strain was

found to be sensitive to broad spectrum antibiotics
which was reported and recommended to the T.V.C.C.
for their administration in divided doses on alternate
daily intervals in mixed preparations.
ACKNOWLEDGEMENTS
The authors are thankful to Honble Dean and
Management (Hony. Chairman and Secretary, Aastha
Society, Sikar) of Arawali Veterinary College, Sikar for
providing the necessary facilities to carry out this
research work.
REFERENCES
1.
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3.
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9 References
Ananthanarayan, R. and Paniker, C.K. Jayaram. 2009.

Textbook of Microbiology. 8thed. Universities Press (India)
Pvt. Ltd. Hyderabad, India. ISBN 978 81 7371 674 4.
Buxton, A. and Fraser, G. 1977. Animal Microbiology. Vol.
1. Blackwell Scientic Publications.
Cruickshank, R., Duguid, J.P., Marmion, B.P. and Swain,
R.H.A. 1975. Medical Microbiology. 12th edn. Vol. II,
Churchill Livingstone, London.
Finegold, S.M. and Martin, M.J. 1982. Diagnostic
Microbiology. 6th ed. The C.V. Morsby Co., London.
Ganguly, Subha. 2014. A comprehensive and illustrious
review on clinical and diagnostic aspects of Mastitis
infection in high yielding lactating cows. World J. Pharma.
Res. 3(9): 352-60.
Kumar, Mayur, Prasad, Arun, Tiwary, B.K. and Ganguly,
Subha. 2010. Study on incidence of mastitis in cattle
population of Ranchi (Jharkhand) under dierent dairy farm
conditions. Livest. Line, 4(6): pp. 8.
Patnaik, Subhasree, Prasad, Arun and Ganguly, Subha.
2013. Mastitis, an Infection of Cattle Udder: A Review. J.
Chem. Biol. Physical Sci., Section-B [Biological Sciences]
3(4): 2676-8.
Patnaik, Subhasree, Prasad, Arun and Ganguly, Subha.
2014. Biochemical characterization and antibiogram of
Staphylococcal microorganisms associated with subclinical
mastitis in lactating crossbred cows. Anim.
Rep.,
PeopleSci.
who
read8(4):
this publication also read:
123-9.
Paul, I., Isore, D.P., Joardar, S.N., Mukhopadhayay, S.K.,
Article: [Qualitative
evaluation of the results of a method proposed for
Ganguly, S. and Pal, S. 2013.Bacteriological
investigation
and antibiogram on Methicillin-resistant
Staphylococcus
standardization
of the bacteriologic...
aureus (MRSA) causing subclinical mastitis in dairy cattle
Jul 1977
population of West Bengal. Indian J. Comp.
Microbiol.
Immunol. Infect. Dis. 34(2): 56-9.
Share
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International Journal of Medical Microbiology and Tropical Diseases, October-December, 2015;1(1):6-7

Jul 1977
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Citations
References
Textbook of Microbiology
Article Jan 1978 World Journal of Pharmaceutical Research
Ananthanarayan
Paniker
Read
Diagnostic Microbiology
Article Jan 1982 World Journal of Pharmaceutical Research
S.M. Finegold
E.J. Baron
Read
A COMPREHENSIVE AND ILLUSTRIOUS REVIEW ON CLINICAL AND

DIAGNOSTIC ASPECTS OF MASTITIS INFECTION IN HIGH YIELDING
LACTATING CATTLE
[Show abstract]
Full-text Article Nov 2014

Subha Ganguly
Read full-text
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Bacteriological Examination and Antibiogram of Milk Sample of

specication of the manufacturer required for

Bhiwadi, Rajasthan, India). The concentration of

Bhiwadi, Rajasthan, India). The concentration of

The present study revealed the presence of

International Journal of Medical Microbiology and Tropical Diseases, October-December, 2015;1(1):6-7

People who read this publication also read:

Download Full-text PDF

Subha Ganguly et al.

Bacteriological Examination and Antibiogram of Milk Sample of Clinically.

clinical mastitis in dairy cattle. The bacterial strain was

Ananthanarayan, R. and Paniker, C.K. Jayaram. 2009.

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International Journal of Medical Microbiology and Tropical Diseases, October-December, 2015;1(1):6-7

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A COMPREHENSIVE AND ILLUSTRIOUS REVIEW ON CLINICAL AND

Full-text Article Nov 2014

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[Qualitative evaluation of the results of a method proposed for standardization

Article: [Qualitative evaluation of the results of a method proposed for

Article Jul 1977

[The antibiogram and the bacteriological examination in antiboitic therapy of

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Article May 1962

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