E. W. CBAMPTON
of nutrition,
Macdonald College, itcGill
P.O., Prov. Quebec, Canada
university,
PIVE FIGURES
Macdonald
491
Copyright, 1947
The Wistar Institute of Anatomy and Biology.
All rights reserved.
This
One
WQC6-KP4-PJYF
492
E.
W.
CRAMP-TOST
VITAMIN
C BIOASSAY
493
494
E. W. CEAMPTOX
Successful
pregnanciesHemorrhage
fetusAbortionNumber
and rsorptionof
WITH FRESH
OR DRIED
ROUGHAGE
-13%3.73.03.0100
gm260
gm30
gm275
gm43
gm80%20%0%3.93.53.5100
gm
VITAMIX
C BIOASSAY
495
ir- *T-~ TI
'^i\A
0.5 mg
1.0 mg
2.0 mg
Fig. 1 Photomicrographs
(X440)
showing development of the odontoblast
cells in guinea pig incisor teeth resulting from the daily intake of 0.5, 1.0, and
2.0 mg of ascorbic acid, respectively. P, o, and d indicate pulp, odontoblasts and
dentine, respectively.
496
E. W. CRAMPTON
?'.
.: *'''.'.
'.'" ' '
.> ;
';. '
- ~ -"'.-?';
' .'
''
--VjaA.:.-.- -
-
Fig. 2 Photomicrograph
(X 100) showing irregular odontoblast row in a
scorbutic tooth. (P) indicates disorganized pulp, (es) calcifiscar tissue, (o) de
generating odontoblasts, and (d) dentine, respectively.
Fig. 3
Formative
VITAMIN
C BIOASSAY
497
Fig. 4
Mature odontoblast
a
to
C
of
70
of
(P) indicatcs,
498
E.
W.
CRAMPTOX
considerations
VITAMIX
C BIOASSAY
499
pr'
yS
{Q\
b2
500
E.
W.
CRAMPTON
GROWTH METHOD
pr
Aqueous
soinFresh
ascorbicjuicejuice,
orangeCone,
orangeAqueous
soinFresh
ascorbicjuiceSynthetic
orange,
juiceApple orange
juice2.83.55.43.23.23.23.2322159211822331010610101010.039.074.053.070.082.065.04449.356.526.557.657.657.657.624761
1nc in growth assay to give pr equal to odontoblast assay.
2i.e., Growth assay has 44% the precision of the odontoblast assay.
VITAMIN
C BIOASSAY
501
502
E. W. CRAMPTON
alcohol20%
alcohol40%
alcohol60%
alcohol80%
alcoholAbsolute
alcoholAbsolute
alcoholOedarwood
oil52
paraffin60"C.
C.
paraffinIN9:00
a.m.12:
noon5:00
00
p.m.9:00
a.m.12:
noon5:00
00
p.m.9:
a.m.10:30
00
a.m.12:
noon2:
00
30 p.m.changing
noon5:
00
p.m.9:
00
a.m.12:
00
noon5:
00
p.m.9:00
00
a.m.10:30a.m.12:00
noon2:30
p.m.4:30
p.m.
onceOUT12:
VITAMIN
C BIOSSAY
503
treatment
of the data
CITED
BLISS, C. I., ANDH. P. MARKS 1939 The biological assay of insulin. II. The
estimation of drug potency from a graded response. Quart. J. Pharm.
and Pharmacol., 12: 182.
504
E.
W.
CEAMPTOX