Course Description: This course deals with the basic concepts of immunology and principles of serological
procedures, reading, interpretation of results and their clinical significance.
Credit Units:
Contact Hours:
Prerequisite:
Placement:
Course Objectives:
At the end of the course, the student should be able to:
1. Explain the immune system of the body
2. apply the principles of immunology in serology and laboratory diagnosis of specific disease through the use of
serological reactions
3. appreciate the importance of the course in the diagnosis and treatment of diseases
4. manifest the following values: Integrity. honesty, critical thinking, empathy and value for life
Course Outline:
Part 1: IMMUNOLOGY
UNIT 1: INTRODUCTION
1. Historical Development
2. Definition of Terms
UNIT II: IMMUNITY
1. Natural/Innate Immunity
o First line of Defense
- Anatomical/ Physical Barriers of Infections
o Second Line of Defense
- Physiological Barriers
- Biochemical Factors
- Cellular Factors
- Phagocytosis
o Third Line of Defense
- Immune response
2. Acquired/Adaptive Immunity
2.1. Active Acquired Immunity
2.2. Passive Acquired Immunity
2.3. Humoral Immunity
2.4. Cell-Mediated Immunity
2.4.1. Cytokines
UNIT III: THE IMMUNE SYSTEM
1. Phylogeny of the Immune System
2. Anatomy Compartments of the Immune System
3. Anatomy and Physiology of the Lymphoid Organs
4. Cells of the Immune System
Ontogeny of the Lymphoid Cells
Characteristics and Functions of the Lymphoid Cells and
other cells involved in the Immune response
UNIT IV. ANTIGENS
1. Structure and Biologic Properties of an Antigen
3. Factors Affecting Immunogenecity
UNIT V. ANTIBODIES
1. Biologic Structure and Functional Properties of
Antibodies
2. Classification of Antibodies
3. Enzymatic Fragmentation and Reduction of An
Antibody Molecule
4. Theories of Antibody Synthesis
5. Immunoglobulin Genetics
6. Antibody Diversity
7. Monoclonal Antibody Production
UNIT VI. MAJOR HISTOCOMPATIBILITY
COMPLEX
1. MHC Cluster of Genes: Characteristics and
Importance
2. MHC Genetic Regions
H2 Complex Map
HLA Complex Map
3. HLA Classes of Molecules
4. HLA Testing and Their Application
UNIT VII: COMPLEMENT SYSTEM
1. Major Proteins of the Complement System
2. Pathways of Activation
3. Biologic Consequences of Activation
4. Measurement of its Biologically Active Components
UNIT VIII: BIOLOGY OF THE IMMUNE RESPONSE
1. Fate of the Antigen
2. Phases of Immune response
3. Structure and Functions of the T cell Receptor (TCR)
4. T and B cell Activation Process
UNIT IX: IMMUNOMODULATION
1. Immunoprotentiation
2. Immunosuppression
3. ImmuneTolerance
UNIT X: ASSAYS OF IMMUNECOMPETENCE
1. Enumeration and Functional Assays of T cells
2. Enumeration and Functional Assays of B Cells
3. Assays on the Functions of Phagocytes
UNIT XI: HYPERSENSITIVITY REACTIONS
1. Types of Hypersensitivity
2. Laboratory Methods
UNIT XII: TRANSPLANTATION
1. Terminologies used in Transplantation
1.1.Types of Graft
2. Host response to Transplantation
2.1. Host-versus-Graft reaction
2.2. Graft-versus-Host reaction
Laboratory Exercises:
1. Anatomical body defense- skin
2. Chemical body defense- effect of normal human
serum on certain Species of bacteria
3. Phagocytosis
4. Organs and Cells of the immune system
5. Preparation of Antibodies- Antiserum in rabbits
6. Preparation of Serial Dilution
7. Flocculation-VDRL, RPR
8. Precipitation by Ring method
9. Immunodiffusion
10. Febrile Agglutination Tests-Widal Test, Weil Felix
test
11. CRP Latex Agglutination test
12. RF Latex Agglutination test
13. ASO Latex Agglutination test
14. HBs One Step Serum test (w/emphasis on the
mechanism of reaction)
15. Particle Agglutination Test for Anti-HIV
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Prepared by:
Jzyl Anne D. Pedronio, R.M.T.
Lecture Instructor
Approved by:
Arturo Marcelino Jr., R.M.T
MLS- Program Coordinator
Prepared for:
3rd Year, 2nd Semester
A.Y. 2016-2017
LABORATORY: 40%
50%
QUIZZES
30%
CLASS STANDING
S H A R P-
20%
Seatwork/ Activities
Homework
Attendance
Recitation/ Reporting/Interview
Participation
2.5%
2.5%
2.5%
10%
2.5%
________________
100
Prepared by:
Approved by:
LABORATORY: 40%
50%
25%
25%
QUIZZES
CLASS STANDING
S H A R P-
15%
35%
Seatwork/ Activities
Homework
Attendance
Recitation/ Interview
Participation
10%
5%
5%
10%
5%
________________
100
LABORATORY REQUIREMENTS:
Individual:
1. Laboratory PPE (Personal Protective Equipment)
a. Laboratory Gown
d. Hair Net
b. Goggles
e. Face Mask
c. Gloves
f. Close Duty/ School shoes
2. Seatwork/ Activities
a. Colored Folder
c. Art Materials
b. Long-Sized Bond Paper
3. Recitation/Interview Requirements
a. 1 x 1 picture
b. Index Card
4. Written Requirements
a. BLACK ORDINARY PEN should ALWAYS be used in answering any written requirements.
b. Written output should be written legibly, no cursive/script and/or PRINTED.
5. Group:
1. Maintenance of cleanliness and neatness
a. Each group should have a maintenance kit which should include the following:
i. Tissue paper
iv. Cloth/ Towel
ii. Alcohol
v. Laundry Soap
iii. Hand soap
vi.
vii.
viii.
ix. Prepared by:
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xii.
xiii. John Christian Rubino, R.M.T.
Marcelino Jr., R.M.T.
Approved by:
Arturo
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xx. SCHEDULE AND COVERAGE:
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xxii. IMMUNOLGY AND SEROLOGY LECTURE: Wednesday, 12:00 NOON-3:00 PM
xxiii. IMMUNOLGY AND SEROLOGY LABORATORY: Monday, 1:00 PM-4:00 PM
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xxvii. TOPICS
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lxxxvii. 8. Precipitation by Ring method
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xcv. 10. Febrile Agglutination Tests-Widal Test, Weil Felix test
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clviii. Prepared by:
clix.
clx. Jzyl Anne D. Pedronio, R.M.T.