A new allograft for eyelid repair

A. Yu. Salikhov, E. R. Muldashev, R. T. Bulatov, N. D. Kulbaev

The Association for Research in Vision and Ophthalmology
Abstract Book Annual Meeting
Fort Lauderdale, Florida
May 14-May 19, 1995
p.347-255

Purpose. To develop an allograft for eyelid repair to restore the eyelid tarsus, to prevent postoperative ectropion and entropion.
Methods. Histological (62 rabbits), immunological, clinical studies (1014 patients with eyelid carcinoma, ectropion, entropion, coloboma, deformations, etc. followed-up for 1-6 years).
Results. The dermal layer of skin with high content of hyaluronic acid, processed under the
Alloplant technology (E.R.Muldashev et al., 1987, 1988) has mechanical features providing tarsus
restoration and can serve as a frame for the eyelid, thus preventing entropion or ectropion.
Allogratfs of the mentioned tissues have low immunogenecity, are gradually replaced by compact
recipient tissue and can be used in various types of eyelid surgery. A randomized group of 262
patients followed up for 1-6 years after operation have proved the treatment to be positive in 74%
of cases.
Conclusion. The allogratfs of skin derma produced according to the Alloplant technology can be
recommended as a suitable material for eyelid tarsus restoration.

Regeneration of cornea in alloplant keratoplasty

Nigmatullin R.T., Muldashev E.R., Muslimov S.A.
8-th International Meeting on Cataract.
Bali (Indonesia).
1995 . P60

It was a well known fact, that lamellar keratoplasty depends on availability of donor corneas. Due
to national, religious and legal reasons, the use of donor corneas is difficult and sometimes even
impossible. The Center has developed a new biomaterial Alloplant that was patented in Russia and
USA. Alloplant for lamellar keratoplasty is produced from the human cadaveric tendon with the
concentrated content of keratan sulfate. This material is semitransparent. Alloplant keratoplasty
was performed in 106 rabbits.The implanted Alloplant and the cornea bed have been studied in 7360 days period with the morphologic methods and the quantitative polarization methods specially developed in the Center. It was found out, that Alloplant was replaced with recipients cornea tissue in three reparative steps. In 7-30 days period Alloplant became homogeneous, fragmentary and
the anisotropic coefficient increased. Then in 90-360 days period new corneal plates were formed
and replaced Alloplant. The structure and optic properties of the regenerate was identical to those
of the host cornea. No ngiogenesis was observed in the studies. The resultsobtained allow to recommend Alloplant to be used in clinic in therapeutic, barrier and optic keratolasty.