Clinically Tested
Toxicologically Tested
C.S.I.R.
Council of Scientific
& Industrial Research
Converts
proinsulin to insulin
Vijaysar
Rich in flavonoids ,
strengthens the cells
and help maintain
normal blood
glucose level
Giloy
A unique herb to
improve immunity.
Helps improve
resistance to
infections
Exerts
Anti-oxidant Action
BLOOD GLUCOSE
METABOLISM
and Restores Quality of Life
Majeeth
Powerful anti-oxidant
activity. Helps protect
vital organs from
oxidative damage
Methika
One of the
best sources of
Micro-nutrients.
Nourishes & tones
the vital organs
Gudmar
Maintains
post-prandial
blood glucose level
by delaying glucose
absorption
Dosage : Tablets : 2 tablets twice a day, Granules: teaspoonful twice a day, half
half an hour before meals
an hour before meals
or as directed by Physician.
BREAKTHROUGH PHYTORESEARCH
of the Decade
Reduces level of
glycosylated Hb
JOINTLY DEVELOPED BY
THE SCIENTISTS OF
CENTRAL INSTITUTE OF
MEDICINAL & AROMATIC
PLANTS, (LUCKNOW)
BLOOD
GLUCOSE
REGULATOR
TO MANAGE THE LIVES OF SUFFERING DIABETICS
funs'kd
Dr. Chandra Shekhar Nautiyal
TATA Innovation Fellow, FNA, FNASc, FNAAS
Director
FOREWORD
Insulin
Glucose
Insulin Receptor
Glucose
Channel
Normal
body cell
Body
cell with
Diabetes
Increased Blood
Glucose Level
Manages glucose
absorption &
uptake
Supplements bioactive
constituents like
galactomannan, reduces
post prandial glucose level
because of its property to
delay intestinal absorption
of glucose and benefits in
blood glucose management Trigonella foenum-graecum
(Methi)
What is special
about BGR-34?
BGR-34 is a novel,
natural DPP-4 inhibitor
and unlike other DPP-4
inhibitors in market, BGR34 exerts cardioprotective
action. It also exerts
powerful anti-oxidant
action, helps prevent
development of triopathic complications. An
optimized concentration
of synergistically acting
extracts makes BGR-34
highly efficacious in
management of
Diabetes.
C.S.I.R.
Effectively controls diabetes by inhibiting DPP-4
(GLP-1 & GIP)
1066, 2009 )
DPP-4 on brush
border of intestine
GLP-1
GIP
Retards glucose
absorption
Controls
Blood Sugar Level
80
% Inhibition
Increases
Glucose uptake
& Storage by
muscles
Increases
insulin secretion
Enhances
Insulin sensitivity
% Inhibition
Decreases glucose
production by liver
(Decreases gluconeogenesis)
Reduces feeling
of hunger
(Increases satiety)
60
40
20
0
-1.0
80
60
40
20
0
-1.5
0.0
0.5
1.0
Log Conc.
0.0
0.5
1.0
1.5
2.0
Log Conc.
Figure-2: DPP-4 inhibitory activity of Berberis aristata ext.
Converts Pro-insulin
to insulin
C.S.I.R.
Repairs & Revives -cells, Enhances Insulin Release
A study conducted with Pterostilbene, a constituent derived from Pterocarpus marsupium
(Vijaysar) showed hypoglycemic activity in experimental subjects because of presence of tannates
in the extract. Marsupin, pterosupin and liquiritigenin obtained from
vijaysar showed antihyperlipidemic activity. ()Epicatechin, its active
Flavonoid
principle, has been found to be insulinogenic, enhancing insulin release
fraction from
by converting proinsulin to insulin. Like insulin, () epicatechin
stimulates oxygen uptake in fat cells and tissue slices of various organs,
Pterocarpus
increases glycogen content of experimental subjects diaphragm
marsupium (Vijaysar)
in a dose-dependent manner.
Pancreas
HbA1c (%)
Drug
Group
Baseline
Vijaysar
(n=45)
10.5
Tolbutamide
(n=45)
10.5
Parameter
At 36
weeks
8.9
8.7
Mean fall
(95%C.I)
Fasting
1.6*
1.8*
Postprandial
Vijaysar
(Pterocarpus marsupium)
A rich source of
(-)epicatechin
Repairs
& Revives
-cells
of islets
of Pancreas
Increases
glucose
mediated
insulin
secretion
Increases
glucose
utilisation
in tissues
Drug
group
At
Baseline
At 36
weeks
Mean fall
(95%C.I)
Vijaysar
(n=172)
9.4
7.0
2.4
Tolbutamide
(n=177)
9.4
6.7
2.7
Vijaysar
(n=172)
13.9
9.6
4.3
Tolbutamide
(n=177)
13.8
9.4
4.4
Helps
Control
Diabetes
Mellitus
Weight
Plasma Glucose
Triglycerides
Blood Glucose
Concentration
200
150
100
Reduces intestinal
absorption of glucose
Trigonella foenum-graecum (Methi) seeds
contain 45% to 60% carbohydrate
( ga l a c to m a n n a n ) , 6 % to 1 0 % l i p i d
(polyunsaturated fatty acids), and 20% to
30% protein (4-hydroxyisoleucine) being
one of the major amino acids.
Galactomannan, a polysaccharide polymer
that dissolves in water because of the
presence of galactose side chain, has been
reported to reduce postprandial blood glucose
response because of its viscous property and has
the potential to reduce intestinal absorption of low
concentrations of glucose and benefit in blood glucose
management.
(Nutrition res., 29, 49-54, 2009)
50
STD
Control
HFD
Control
TFG
Treated
60
50
40
% Inhibition
70
30
20
10
0
50
100
150
200
Concentration (g/ml)
250
Aqueous extract
C.S.I.R.
40
30
20
10
0
50
100
150
200
Concentration (g/ml)
250
Aqueous extract
C.S.I.R.
Strengthens - cells functional capacity
Regulate glucose
homeostasis
Decreases
gluconeogenesis
Increases glucokinase
& G-6PD activity
Decreases G-6P activity
Protects from oxidative
stress
Gymnemic acid is the main active constituent of Gymnema sylvestre possessing beneficial effects
in controlling blood glucose level by strengthening -cells.
Gymnema sylvestre
(Gurmar)
Promotes repairative
regeneration of islet cells.
Increases secretion of glucose
mediated insulin.
Causes delay of glucose
absorption from intestine.
Increases the utilization of
glucose as it increases the
activities of enzymes
responsible for utilization of
glucose by insulin-dependent
pathways.
Increases in phosphorylase
activity, decreases in
gluconeogenic enzymes and
Sorbitol dehydrogenase.
Berberis aristata
(Daruharidra)
The steptozocin induced diabetics had a damage over pancreatic acinar region as can be seen by reduced
pancreatic amylase and lipases and these decreases are corrected by Gurmar therapy.
Groups time
Evaluation
st
SGPT
BGR-34
Placebo
SGOT
p value
Hb
I week
line)
Group(base
1 (NC)
204.6833.4
31.82+5.18
BGR-34
194.5612
27.51+2.16
13.4+1.59
0.42
th
IV Week
Group
2 (NE)
203.3512.6
30.20+6.29
BGR-34
192.3114
28.55+3.12
14.1+2.26
0.48
th
VIII week
Group
3 (DC)
200.1528.4
65.78+7.29
BGR-34
191.0018
61.56+3.41
12.5+1.82
0.36
th
XII week
Group
4 (DE)
197.6216.4
48.58+4.29
BGR-34
190.1822
39.69+2.89
13.3+1.72
0.41
th
XVI week
Group
5 (DG)
192.3923.8
45.34+3.69
BGR-34
188.2424
45.44+5.01
12.9+1.79
0.51
Values are given as mean S.D for five determinations in each group.
NC: Normal control, NE: Normal rats administered Rubia cordifolia ,
DC: Diabetic control, DE: diabetic experimental rats administered
Rubia cordifolia, DG: Diabetic rats administered glybenclamide
C.S.I.R.
Primary screening of anti-diabetic potential of the herbal combinations in different proportions was
undertaken by conducting Oral Glucose Tolerance Test (OGTT) as per the selected parameters. The treatment
groups were dosed orally at 2000mg/kg body weight, 30 min. before giving glucose solution. Group 1 of
experimental subjects was treated as normal control, and given only glucose solution orally. Group 2 referred
as positive control, was treated with Metformin at 250 mg/kg body weight. Group 3-9 were given different
combinations of herbs. The blood glucose level at intervals of 15, 30, 60 and 90 min were recorded after giving
glucose solution successively. Based on the glucose metabolism kinetics of combinations, BGR-34 was found
to have most optimum composition with best of anti-hyperglycemic activity.
"
BGR-34, delays in
absorption of
glucose from GIT,
Inhibits Advanced
glycation
end products (AGEs)
accumulation,
enhances insulin
release & increases
conversion of
pro insulin to
insulin.
"
Glucose (mg/dL)
275
Vehicle
Metformin
I
225
(2:2:0)
BGR-34
150148146144142140138136134132-
FBS 1
FBS 16
210205200195-
II (2:1:0)
III (2:0:1)
175
IV (2:0:2)
125
V (2:0:5:0:5)
VI (2:0:0)
75
0
15
30
45
60
75
90
VII (2:1:1)
105
25
Conclusion:
15
30
60
90
Group-1 Vehicle
899.03
BGR-34
20220.85
BGR-34
215.714.65
BGR-34
1319.97
BGR-34
85.53.41
BGR-34
Group-2 Metformin
92.44.92
BGR-34
151.310.41
BGR-34
138.85.90**
BGR-34
109.15.88
BGR-34
80.64.67
BGR-34
Group-3 I (2:2:0)
70.28.03
BGR-34
161.629.53
BGR-34
11724.74***
BGR-34
52.69.34***
BGR-34
65.14.07
BGR-34
Group-4 II (2:1:0)
80.85.32
BGR-34
145.236.35
BGR-34
128.67.88***
BGR-34
89.47.10***
BGR-34
75.33.70
BGR-34
47.53.03
BGR-34
147.413.89
BGR-34
126.610.21***
BGR-34
76.610.46***
BGR-34
71.64.80
BGR-34
Group-6 IV (2:0:2)
56.86.10
BGR-34
148.817.94
BGR-34
123.615.15***
BGR-34
773.18***
BGR-34
59.751.65
BGR-34
Group-7 V (2:0.5:0.5)
NBRMAP-DB
56.52.67
BGR-34
183.616.70
BGR-34
135.64.82***
BGR-34
665.68***
BGR-34
58.61.40
BGR-34
Group-8 VI (2:0:0)
46.36.59
BGR-34
115.7510.59
BGR-34
104.2512.65***
BGR-34
60.53.48***
BGR-34
63.253.09
BGR-34
86.45.18
BGR-34
90.36.20**
BGR-34
625.41
BGR-34
138.26.91
BGR-34
117.75.91***
BGR-34
190185PPBS 1
PPBS 16
180- Fig 2: Comparison between means of Post Prandial Blood
Sugar (PPBS) mg % values in drug and placebo arms
before and after the treatment
8.07.87.67.47.27.0HbA1c 1
6.8-
HbA1c 16
ALP (IU/L)
200 ***
150 -
***
100 50 0Vehicle
Metformin
BGR-34
Streptozotocin
(NBRMAP-DB-II)
***
***
Vehicle
Metformin
BGR-34
Streptozotocin
(NBRMAP-DB-II)
#
*
50 -
40 30 20 10 0-
Vehicle
Metformin
BGR-34
(NBRMAP-DB-II)
Streptozotocin
Group1
Bio-chemical
Parameters
Vehicle
BGR-34
Group 2
Metformin
Group 3
BGR-34
(NBRMAP-DB-II )
Group 4
Streptozocin
ALP(IU/L)
52.61
BGR-34
3.24
Creatinine (mg/dl)
0.36
BGR-34
0.02
0.29 0.03
0.40 0.03***
0.69 0.02#
TC (mg/dl)
33.46
BGR-34
2.80
39.59 2.28*
37.18 1.42*
51.15 4.6#
The values are expressed as mean SEM. n = 6 experimental subjects per group. Significant
difference of diabetic control from normal control on consecutive days: #P < 0.001. Significant
difference of treated groups from diabetic control on the corresponding days: * P < 0.05,
** P < 0.01, *** P < 0.001
"
"
Rubia cordifolia
2. Berbamine
3. Palmatine
Insulin secretagogues
Gymnema sylvestre
Tinospora cordifolia
Pterocarpus marsupium
Berberis aristata
C.S.I.R.