Research Article
*Farah Amna Othman , Nooraain Hashim , Noriham Abdullah , Azizah Abdul Hamid , Mohamad Faiz Foong Abdullah , Zainon Mohd Noor , Normah
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1
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Ismail , Wan Rozianoor Mohd Hassan , Nurul Husna Rosli
1
Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.
2
Institut Agro-Bioteknologi (ABI), MARDI, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
INTRODUCTION
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Int. J. Pharm. Sci. Rev. Res., 26(2), May Jun 2014; Article No. 08, Pages: 39-43
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Int. J. Pharm. Sci. Rev. Res., 26(2), May Jun 2014; Article No. 08, Pages: 39-43
Table 1: Haematological values of sub acute treatment with A. bilimbi fruit ethanolic extract of male rats
Treatment Groups
WBC (x10 )
12.222.73
42.003.38
10.532.46
36.833.11
14.013.33
Control
9.441.33
125 mg/kg
8.760.50
250 mg/kg
8.640.41
500 mg/kg
RBC (x10 )
9.850.28
PCV (%)
14.030.37
ab
31.171.33
39.001.65
a, b
MCV (m )
11.672.37
Hb (g/dl)
MCH (pg)
50.4810.18
17.383.85
14.030.37
42.172.82
16.270.97
36.532.45
16.360.69
4.030.37
14.850.01
MCHC (%)
39.691.69
34.643.14
39.072.37
ab
45.331.90
15.150.44
ab
38.451.74
Table 2: Haematological values of sub acute treatment with A. bilimbi fruit ethanolic extract of female rats
Treatment Groups
7.171.80
9.381.61
7.990.52
125 mg/kg
8.690.48
500 mg/kg
WBC (x10 )
Control
250 mg/kg
RBC (x10 )
40.171.78
39.001.90
8.741.98
a
8.640.86
10.001.49
13.750.35
ab
51.675.05
13.480.28
45.272.28
14.030.37
14.850.01
44.172.60
a, b, c
MCV (m )
33.331.67
Hb (g/dl)
8.070.33
PCV (%)
MCH (pg)
MCHC (%)
17.721.67
15.801.10
ab
41.672.77
53.796.92
34.431.08
34.921.61
42.542.16
17.490.68
18.202.06
34.272.22
Figure 1: Cross sections of hearts (H & E staining, 20X). (A): Control, (B): 125 mg/kg, (C): 250 mg/kg and (D): 500 mg/kg
showed normal histopathological features of cardiac muscles, with the nucleui (arrows) were located centrally.
Figure 2: Cross sections of kidneys (H & E staining, 20X). (A): Control, (B): 125 mg/kg, (C): 250 mg/kg and (D): 500 mg/kg
showed intact Bowmans capsules (arrows), glomeruli (arrow) and renal tubules.
Figure 3: Cross sections of livers (H & E staining, 20X). (A): Control, (B): 125 mg/kg, (C): 250 mg/kg and (D): 500 mg/kg
revealed well preserved hepatocytes (arrows) with brought out nuclei and cytoplasm that was also not vacuolated.
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Int. J. Pharm. Sci. Rev. Res., 26(2), May Jun 2014; Article No. 08, Pages: 39-43
Figure 4: Cross sections of rats ovaries (H & E staining, 4X). (A): Control, (B): 125 mg/kg, (C): 250 mg/kg and (D): 500
mg/kg animals revealed normal developing follicles and corpora lutea (arrows).
Figure 5: Cross sections of testes (H & E staining, 10X). (A): Control, (B): 125 mg/kg, (C): 250 mg/kg and (D): 500 mg/kg
showed normal arrangements of the internal structures of the seminiferous tubules. The tubules consist of various stages
of spermatogenic cells and were connected through interstitial space containing interstitial tissues.
Figure 6: Cross sections of rats epididymis (H & E staining, 20X). (A): Control, (B): 125 mg/kg, (C): 250mg/kg and (D): 500
mg/kg showed normal sperm density in epididymal lumen.
Histopathological examination revealed intact cells and
tissue architectures and no gross pathological lesion in all
selected organs of both control and A. bilimbi extract
treated rats (Figure 3-6).
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Int. J. Pharm. Sci. Rev. Res., 26(2), May Jun 2014; Article No. 08, Pages: 39-43
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