A.
B.
C.
D.
2.
The lowest biomass yield in a culture of Escherichia coli will be in
A.
B.
C.
D.
3.
When two populations compete for a single growth limiting substrate in a continuous
fermenter, which organism would not be washed out?
A.
B.
C.
D.
4.
The continuous cultures are not widely used in industry because
A.
B.
C.
D.
5.
The lowest yield of ATP /is in
A.
fermentation
B.
aerobic respiration
C.
anaerobic respiration
D.
6.
Mixing in an anaerobic sludge blanket reactor is due to
A.
B.
C.
swimming of microbes
D.
7.
In batch culture, protogon is produced from peptone during the stationary phase with a yield
of 0.4 protogon mg per g of peptone. If it is to be produced in a chemostat at a dilution rate
of 0.5 h-1 from a medium containing 10 g.l-1 of peptone, then the rate of protogon synthesis
would be
A.
0 g.l-1h-1
B.
0.5 g.l-1h-1
C.
1 g.l-1h-1
D.
2 g.l-1h-1
8.
Formation of end product by Lactococcus lactis will become non-growth associated as lactic
acid accumulates because
A.
B.
C.
cells will redirect ATP to facilitate the diffusion of lactic acid and H + out of the cells
D.
cells will redirect ATP to the active transport of lactic acid and H + out of the cells
9.
Which of the following would not be subjected to the "glucose effect"?
A.
Aspergillus fumigatus
B.
Saccharomyces cerevisiae
C.
Escherichia coli
D.
Aspergillus niger
10.
Immobilized cell reactors for wastewater treatment have the advantage of having/being
A.
B.
C.
D.
11.
Which of the statement is true for continuous reactor at steady state?
A.
B.
C.
D.
12.
A continuous bioreactor in which only the flow rate is used to control the rate of cell or
product productivity is called
A.
turbidostat
B.
chemostat
C.
level stat
D.
pH
13.
For organisms growing in a chemostat, the specific growth rate
A.
cannot be determined
B.
C.
D.
14.
Saccharomyces cerevisiae is being grown in a chemostat converts glucose to biomass,
ethanol, glycerol and carbon dioxide. At steady state, the concentration of glucose, biomass,
ethanol and glycerol will
A.
B.
C.
be constant
D.
15.
In aerobic yeast fermentation for production of citric acid from alkanes using a fed-batch
A.
B.
C.
Fast addition of alkanes will inhibit the cells and reduce oxygen transfer rates
D.
Fast addition of alkanes will cause the cells to grow too quickly
16.
A continuous reactor has a dilution rate of 0.5 h -1. Its residence time would be
A.
ln(2)/0.5
B.
ln(2) x 0.5
C.
0.5 h
D.
2h
17.
Which of the following is used to calculate mass of substrate in the reactor?
A.
B.
C.
D.
18.
If biomass yields are constant, then the biomass productivity of a culture grown in
continuous reactor will
A.
B.
C.
D.
19.
Low dissolved oxygen concentrations leads to
A.
B.
C.
D.
20.
In a continuous reactor, the medium contains 40 g.l -1 of maltose and the medium flow rate is
10 litres per hour and the effluent contains 20 g.l -l of lactate. What is the productivity of
lactate production from this reactor?
A.
50 g maltose.l-1
B.
50 g lactate.l-1
C.
200 g maltose.l-1
D.
200 g lactate.l-1
21.
A fed-batch reactor containing 1 g.l-1 of substrate in 2 litre of medium. It is fed with 1 g.l-lof
substrate at the rate of 1 litre per hour. After 10 hours, the concentration of substrate in the
reactor was 0.5 g.l-1 . The mass of substrate that was used by the culture in the reactor was
A.
2g
B.
6g
C.
8g
D.
12g
22.
Toxins accumulated in a reactor can lead to low biomass yields probably due to which of the
following?
A.
B.
C.
D.
23.
The cellular productivity in a continuous stirred tank fermenter (CSTF) increases with an
increase in the dilution rate and reaches a maximum value. If the dilution rate is increased
beyond the maximum point, the productivity will
A.
decrease abruptly
B.
increase
C.
increase drastically
D.
be zero
24.
Fluidized bed bioreactors provide higher mass transfer rates than packed bed bioreactors
because
A.
B.
C.
D.
25.
In fed-batch fermentation, mass of substrate utilized is calculated using the relationship
A.
initial mass of substrate in the reactor + final mass of substrate in the reactor +
flow rate x [substrate in the feed]
B.
initial mass of substrate in the reactor - final mass of substrate in the reactor +
flow rate x [substrate in the feed]
C.
initial mass of substrate in the reactor + final mass of substrate in the reactor -
D.
initial mass of substrate in the reactor + final mass of substrate in the reactor flow rate
26.
A chemostat has a liquid volume of 2 litres and is being fed at a rate of 4 litres per hour.
Dilution rate for this reactor will be
A.
2 litres
B.
C.
2 h-1
D.
27.
Mixing per unit volume is observed to be poorest in
A.
B.
C.
D.
28.
The growth of an organism on glucose is described by the following Monod model
parameters: m = 0.5 h-1 and Ks =0.1 g.l-1, if the concentration of glucose in the feed is 10
g.l-1 and the dilution rate is set to 0.4 h-1, then the steady state concentration of glucose in
the effluent will be
A.
0 g.l-1
B.
0.5 g.l-1
C.
1.0 g.l-1
D.
10 g.l-1
29.
A continuous reactor at steady state contains 0.04 g.l -1 of biomass and 0.02 g.l-1 of phenol.
The feed contained 0.1 g.l-1 of phenol. The biomass yield would be
A.
0.4 g.g-1
B.
0.5 g.g-1
C.
1.0 g.g-1
D.
5.0 g.g-1
30.
A culture system with constant environmental conditions maintained through continual
provision of nutrient and removal of wastes is called __________ culture system.
A.
continuous
B.
batch
C.
fed-batch
D.
semi continuous
31.
The main reason for production of antibiotics in fed batch reactors is
A.
B.
C.
D.
32.
Fed batch reactors is used to produce vinegar because
A.
B.
C.
D.
33.
When a culture of fermenting yeast becomes metabolically uncoupled, ethanol
A.
B.
C.
D.
34.
Mixing profiles closest to plug flow are observed in
A.
B.
C.
D.
all of these
35.
On a glucose medium, the growth of an organism is characterized by the following Monod
model and stoichiometric parameters m = 0.3 h-1, Ks =0.05 g.l-1 and Yxs = 0.3 g.g-1When
this organism is grown in a 2 litre continuous on a medium containing 10 g.l -1 of glucose
added at 1 l.h-1, the steady state concentration of biomass in the reactor will be
A.
0 g.l-1
B.
0.4 g.l-1
C.
0.5 g.l-1
D.
10 g.l-1
36.
Unsteady state mass balance for dynamic model of continuous stirred tank reactor (CSTR) is
A.
time rate of total amount in rector = rate of addition to reactor -rate of removal +
rate of formation
B.
C.
rate of total amount in rector = rate of addition to reactor - rate of removal + rate
of formation
D.
37.
A fed-batch reactor initially contains 2 litre of medium. If it was fed at 1 litre per hour, then
after 10 hours, the volume of the reactor will be
A.
1 litre
B.
2 litre
C.
3 litre
D.
13 litre
38.
A Bacillus sp. produces an antibiotic only during the stationary phase when grown in a batch
culture. If grown in a 5 litre continuous culture, the steady state productivity of the antibiotic
A.
B.
C.
will be zero
D.
will be infinite
39.
In a fluidized bed reactor, the function of the disengagement zone is to
A.
B.
reduce the velocity of the particles near the surface of the reactor
C.
D.
40.
Saccharomyces cerevisiae produces high biomass yields at low glucose concentrations and
high dissolved oxygen concentrations. Which of the following should be followed for
maximizing its biomass productivity?
A.
B.
C.
D.