In Lab 2 experimental session, the compound light microscope (manufactured by

_____) was used for viewing cellular structure and collecting the data on cellular size
in prokaryotes (names) using staining and sterile techniques. For high
magnification, an oil immersion lens was used.
For reviewing prokaryote name, a drop of 1% NaCl was placed on the center of the
sterile glass slide. The bacteriological loop was used to mix the bacterial with the
1% NaCl on the slide and spread to roughly the size of a dime. After the smear was
dry, the area was covered with 95% methanol to fix the bacteria. After the methanol
dried, a drop of crystal violet stain was added on it and after one minute exes stain
was rinsed with distilled water. The sample then was viewed using light microscope
100X objective.
For reviewing prokaryote name, a small drop of congo red stain was added at the
end of the sterile dry slide. The sterile bacteriological loop was used to transfer the
bacterial to the drop of the stain to allow mixing. After the stain was spread evenly
by pulling a short edge of another dry slide along the bottom slide. The stain was air
dried, covered with coverslip and then imaged using the light microscope 100X
objective.
For reviewing Cyanobacteria name, a small drop of the cyanobacteria was placed
on the center of sterile dry slide and was covered with a coverslip. The sample was
then viewed using light microscope 40X objective.