Reference Numbera

JOB SAFETY / HAZARD ANALYSIS

&
RISK ASSESSMENT FORM
UNIVERSITY OF KUALA LUMPUR
(MICET)
Safety data sheets for ALL chemicals used in your project need to be attached WITH
this form.
Once this assessment has been signed by you, a technician, your project supervisor
and safety health officer; an assigned reference number will be given, please submit:

the original document of this assessment to TECHNICIAN IN-CHARGED [TIC] of

main laboratory used; to be kept in the laboratory safety file.

If more than one laboratory used in this project, submit a copy of this document to
the TIC to be kept in the laboratory safety file.

Ensure this assessment is resubmitted for approval when work out of office hour.

Ensure this assessment is read and signed annually by all concerned.

Ensure this assessment is reviewed annually by the project supervisor.
Remember that anything that you put in this form you must follow!

1.

Title of Procedure / Experiment

Extraction of Essential Oil from Christia vespertilionis

as Herbal Supplement
2.

Name of author
HARITH AQWA BIN
KHAIRUSLY
STUDENT ID

Contact Number
0166216713
55103314255

STUDENT EMAIL

3.

aqillah953@gmail.com

Name of Academic Supervisor

Supervisor
Co supervisor

4.

MOHD EDYAZUAN BIN

AZNI
SHARIFAH MARIAM BINTI
SAYED HITAM

Method / Procedure

4.1 Chemicals

Methanol
Aqueous methanol
Anhydrous sodium sulfate

4.2 Materials and Apparatus

4.3

Blender / Grinder
Rotatory Vacuum Evaporator
Distilled Water
Filter paper Whatman No 1
Drying oven
Sealable bag
Universal bottles

Procedure

a) Sample preparation
- Wash the stems and leaves under the running tap water about 5 minutes until it
-

clean and weight it.

Moisture content analysis was performed by spreading moist butterfly wings
leaf on a metal tray and dried at atmospheric temperature and pressure

Butterfly wings leaf then proceed to the blending by cup of cold water for
extraction of juice.

4.2 SAMPLE EXTRACTION

a) Hydrodistillation extraction
Put 100g of sample with 1.0 L distilled into a Clevenger-type apparatus.
The extraction process was carried out for 3 hours.
For the first hour, the collected extracted essential oil are decented from the

condensate at 5 minute intervals.

Next, the extraction is decanting for every 30 minute.
In order to remove the water, the extracted oil is dried in anhydrous sodium

sulfate pellets.
Store the products in universal bottles.
Analyze the sample using HPLC.

b) Soxhlet extraction
Put 100 g of the samples inside the thimble.
Install the solvent on the hot plate in the BUCHI Extraction System.
Put the tube holder on the thimble.
Let the hot plate heat up for five minutes.
Allow the extraction process to take place for 2 until 3 hours.
Collect the produtcs after the extraction finish.
The products contain mixture of methanol and essential oil.
Separate the mixture by using the rotary evaporator in order to obtain pure

essential oil.
Analyze the sample using HPLC.

Type of hazards identified in this project.9

(All hazards must be identified during sampling in & out the campus, performing the experiment in the laboratory and analyzing of
results).

Type of hazards

Table to be completed

Potential Hazards
Yes

Physical hazards

Please fill in table 5a

Biological hazards

Please fill in table 5a

Chemical hazards

Please fill in table 5b

No

/
/
/

5a Hazardsthat have potential to cause harm (potential hazard)

Hazards identified
Handling Electrical Equipment

Broken glassware during experiment

Precautions / Emergency action

Avoid contact with any aqueous solution

Power cable inspection
Do not touch the equipment with wet hand
Treat all circuit as though they were energized
Disconnect electrical equipment before working it
Observe and follow all pertinent instruction and electric warning
Do not touch or operate any device that has a danger or caution tag attached
Do not touch the bare electric wire or connection

Avoid the sharp edges

Handle with care
Ensure there is no broken glasses left while cleaning
Use the first aid

Slip, Trips and Falls

Heat and caustics

Workstation setup

Report the technician on duty

Wear a proper PPE

Wear safety shoes

Keep the floor clean and water free

Report to supervisor if there any needed to non-slipped tilling or other non-slip floor product

Report to the technician on duty

Wear eye protection, goggles and skin protection such as rubber gloves and lab jackets when
conducting the experiments.

Any case of splashes, head over to the emergency shower to rinse the part exposed to the
splashes

Report technicin on duty

Setting up workstation correctly

Make sure there is enough light
Report technician on duty

5b. Substances used which have potential to cause harm (potential hazard)
NB: This section is concerned with hazards inherent in the substances rather than the way they might be used in this procedure. All columns
must be filled according to Safety Data Sheet.

Substance

Hazards identified
(e.g. toxic, flammable)

MEL or OEL (if assigned Precautions in

- do not quote LD50 etc.) handling
(if gloves required state
type)

Methanol

Flammable liquid and

vapor
May cause eye irritation.
May cause mild skin
irritation. Exposure to
high concentrations of gas,
vapor, or mist may cause
irritation.
Chronic ingestion of
ethanol
has
been
associated
with
an
increased incidence of
cancer, liver cirrhosis, and,
if
ingested
during
pregnancy,
congenital
malformations.

Use only in a well-ventilated

area. Avoid breathing vapor or
mist. Avoid contact with eyes,
skin and clothing. When
handling, use appropriate
personal protective equipment
(see Section 8). Wash
thoroughly after handling. Keep
away from heat, sparks, flame
and all other sources of
ignition. Wash hands and any
exposed skin after removal of
PPE. Releases to the
environment should be avoided.
Review and implement
appropriate technical and
procedural waste water and
waste disposal measures to
prevent occupational exposure
or environmental releases.
Potential points of process

Emergency action
in the event of spillage
etc.
Small Spill:
Dilute with water and mop
up, or absorb with an inert
dry material and place in an
appropriate waste disposal
container.
Large Spill:
Flammable
liquid.
Poisonous liquid. Keep
away from heat. Keep away
from sources of ignition.
Stop leak if without risk.
Absorb with DRY earth,
sand
or
other
noncombustible material. Do
not get water inside
container. Do not touch
spilled material. Use water
spray to reduce vapors.

emissions of this material to the

atmosphere should be
controlled with dust collectors,
HEPA filtration systems or
other equivalent controls

Aqueousmethanol

Flammable liquid and

vapor
May cause eye irritation.
May cause mild skin
irritation. Exposure to
high concentrations of gas,
vapor, or mist may cause
irritation.
Chronic ingestion of
ethanol has been
associated with an
increased incidence of
cancer, liver cirrhosis, and,
if ingested during
pregnancy, congenital
malformations.

Use only in a well-ventilated

area. Avoid breathing vapor or
mist. Avoid contact with eyes,
skin and clothing. When
handling, use appropriate
personal protective equipment
(see Section 8). Wash
thoroughly after handling. Keep
away from heat, sparks, flame
and all other sources of
ignition. Wash hands and any
exposed skin after removal of
PPE. Releases to the
environment should be avoided.
Review and implement
appropriate technical and
procedural waste water and
waste disposal measures to
prevent occupational exposure
or environmental releases.
Potential points of process
emissions of this material to the

Prevent entry into sewers,

basements
or confined
areas; dike if needed. Call
for assistance on disposal.
Be careful that the product
is not present at a
concentration level above
TLV. Check TLV on the
MSDS and with local
authorities.
Small Spill:
Dilute with water and mop
up, or absorb with an inert
dry material and place in an
appropriate waste disposal
container.
Large Spill:
Flammable liquid.
Poisonous liquid. Keep
away from heat. Keep away
from sources of ignition.
Stop leak if without risk.
Absorb with DRY earth,
sand or other noncombustible material. Do
not get water inside
container. Do not touch
spilled material. Use water
spray to reduce vapors.

atmosphere should be
controlled with dust collectors,
HEPA filtration systems or
other equivalent controls

Sodium sulfate
anhydrous

Hazardous in case of eye

contact (irritant). Slightly
hazardous in case of skin
contact
(irritant),
of
ingestion, of inhalation.

Do not ingest. Do not breathe

dust. Avoid contact with eyes.
Wear suitable protective
clothing. If ingested, seek
medical advice immediately
and show the container or the
label. Keep away from
incompatibles such as oxidizing
agents, metals.

Prevent entry into sewers,

basements or confined
areas; dike if needed. Call
for assistance on disposal.
Be careful that the product
is not present at a
concentration level above
TLV. Check TLV on the
MSDS and with local
authorities.
Small Spill:
Use appropriate tools to put
the spilled solid in a
convenient waste disposal
container. Finish cleaning
by spreading water on the
contaminated surface and
dispose of according to
local and regional authority
requirements.
Large Spill:
Use a shovel to put the
material into a convenient
waste disposal container.
Finish cleaning by
spreading water on the
contaminated surface and
allow to evacuate through
the sanitary system.

Skin Contact Flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and wash before reuse. Unless
contact has been slight obtain medical attention.
Inhalation Remove to fresh air, rest and keep warm. If breathing is difficult give artificial respiration and obtain medical attention.
Eye contact Irrigate with copious amounts of eye wash or water for at least 15 minutes. Assure adequate flushing by separating the eyelids
with fingers. Obtain medical attention
Ingestion Do not induce vomiting. If conscious provide water for person to thoroughly wash out mouth (and sip if required). Obtain medical
attention.

Full safety data sheets for the above chemicals must available in file at the laboratory in use.
Please ensure data sheets for any previously unused chemical are added to the laboratory's safety file.
Nomenclature
CoSHH:
Control of Substances Hazardous to Health
MSDS :
Material Safety Data Sheet

MEL:
OEL:

Maximum exposure limit

Occupational exposure limit

TWA:

Time weighted average

5b.

Equipment used in the procedure which may cause harm

NB: This section is concerned with hazards inherent in the equipment rather than the way it might
be used in this procedure.

Equipment
Blender/Grinder

Glassware :
Beaker
Filter funnel
Drying oven

Nature of hazard

Precautions to be taken

Electrical hazard

Physical hazard

Thermal

Electrocution

Use guard for utilization

Cables checked for any
damage
Read all instructions
carefully, even if familiar
with the appliances
Never leaver an appliances
unattended when in use
Switch off and remove the
plug from the power when
not in use.
Handles blades carefully to
avoid any injury
Handle equipment carefully.
Wear gloves
Store at safe place

Wear PPE during conducting

experiment
Unplug the oven when not in
use
Ensure the condition of the
oven is good
Handle propely to avoid
accident
Beware of the hot surface of
the oven

NOTE:
ALL EQUIPMENTS USED ELECTRICAL POWER WILL HAVE ELECTRICAL HAZARDS.

5c. Laboratories required

1) Lab 21, Downstream Bioprocessing Lab
2) Lab 13A, Upstream Bioprocessing Lab

5d. First Aid & Fire Fighting Equipment (FAFFE) Available

FAFFE
Eye wash & emergency shower

LABORATORY: 21
Model: HAWS Corporation
Last date checked: 12/3/2015
Condition: GOOD

LABORATORY: 13 A
Model: HAWS Corporation
Last date checked: 2/2/2015
Condition: GOOD

Antiseptic cream
Dressing

Expire: November 2016

Cotton wool
Thermometer
Plaster-Cosmoplast
Medical Gauze Sponge
Gauze Swab
Safety pin
Alcohol pad

Expire: 7/10/2016
Cotton wool
Clinical Thermometer
Plaster-Cosmoplast
Gauze Swabs
Surgical tape
Alcohol Pad
Gauze bandage

Yellow lotion
Sterile pas
Franch oil

Triangular Bandage
Antiseptic Cream
Franch Oil
Burn aid cream
Expire: Not Available
Expire: 02/2016
Expire: 01/2017
Available
Checked on: 2014 /2015
Next checking on:
2015/2016

Eyemo
Yellow lotion
Dettol
Chemical spill kit
Fire Extinguisher

6.

Expire: October 2016

Expire: October 2016
Expire: November 2016
Available
Checked on: 2014 /2015
Next checking on: 2015/2016

Disposal routes for waste materials

Tick [ ] or complete appropriate box for each category of waste WITH reference to FIRST
SCHEDULE of ENVIRONMENTAL QUALITY (SCHEDULED WASTES) REGULATIONS 2005.

Nature of
waste:

Route
Sink

Black
bag

for

disposal:

Black Solvent drum

(SW311;
bin (e.g.
SW410)

SW322; SW323)

Broken glassware

Other route
(specify)

Chemical waste
Gloves, Tissue paper
and Face mask

Sharps
bin

Aquoeous
methanol

NOTE:The following list of scheduled wastes are not exhausted, the above regulation must be
referred to complete this section.
SW109
SW206
SW311
SW322
SW323
SW421

Waste containing mercury or its compound

Spent inorganic acids
Waste of oil or oily sludge
Waste of non-halogenated organic solvents
Waste of halogenated organic solvents
Rags, plastics, papers or filters contaminated with scheduled wastes

NOTE:
PLEASE SPECIFY YOUR BIOHAZARD WASTE - ROUTE OF DISPOSAL

7a. Specific risk assessment for procedure and precautions to be

taken

Full PPE equipment must be worn everytime handling an experiment and in the laboratory.
A spillage mighy occur, so to avoid any clothing, eye and skin contcact with the chemical
and avoid heavy inhalation in the area as well.
Wear glove and mask if necessary, while rhandling acid and alkaline.
Have to handle with care any fragile glassware .
Wash hand before leave the laboratory.
A full alert need to be considered while handling hot equipment. Wear glove to avoid
burning.
Read manual, labels and tags before use any electrical equipment

After following all the above precautions the overall risk assessment for this procedure is LOW.

8.

Level of supervision required and suitability for out-of-hours work

Please mark box [ ] to indicate appropriate supervision category for this procedure.

Category A
Category B
/

Category C
Category D
Category E

work may not be carried out except under the direct supervision of a
specified member of staff who is present continuously
work may not be started without the supervisor's advice and approval, some
additional training and initial direct supervision
work may not be started without the supervisor's advice and approval and
some additional training - no direct supervision required
although extra care must be observed, workers should already be competent
and adequately-trained for this task
risks are insignificant and supervision unnecessary

8a Is this procedure authorised for out-of-hours work ?

YES :___

NO:__/_

[TO BE FILLED FOR WORK TO BE DONE OUT OF OFFICE HOURS]

Date and time

Supervisor Approval

HOS Approval

8b Emergency Contact
No

NAME

CONTACT NO

1.

Security Guard

06-5512054

2.

Safety and Health Officer

019-2436965

Name: NOR AINI BTE BUROK

3.

Supervisor

013-6218161

Name: MOHD EDYAZUAN BIN AZNI

4.

Co-supervisor (if any)

013-9039991

Name: SHRIFAH MARIAM BINTI

SAYED HITAM
5.

Safety and Health Officer

Name: Abdul Hakim BIN HAJI ABU
BAKAR

012-6231203

SHO Approval

Note:
Supervisor is required to make hourly phone calls to check on students when they work out of
office hour.

Assessment reference number 9.

Signature of technician involved in the project.

Form Checked By
(Technician):Form Checked By
(Technician):9a.

Signature
(Date)
Signature
(Date)

Signature of academic supervisor and safety& health officer

Note that this assessment needs to be reviewed and signed annually to take into account any
developments of the procedure [if the project is more than one year, eg postgraduate].

Supervisor (Name):

Signature
(Date)
Signature
(Date)

Co-Supervisor (Name):

9b. Signature of safety & health officer or Safety and Health

Committee member
Safety & Health Officer /SHEC member
Signature
(Name)
(Date)

10. Signature and contact number of workers involved with this

procedure
Please sign annually to say you have read the attached document and understood it.

Personnel
involved
1
3
5
7
9

Status

Signature &
Contact No.
(Date)

2
4
6
8
10

Personnel
involved

Status

11
Assessment reference number

12

Review Section [TO BE FILLED BY POSTGRADUATE ONLY].

11.

Signature of the reviewer/s

Note that this assessment needs to be reviewed and signed ANNUALLY to take into account any
developments of the procedure
(This section should be signed by the technician/s in charge of the areas where the work is to be
done)

Signature
(Date)

Could any less

hazardous substance or
procedure be used?

Signature
(Date)

Could
hazar
proce