Separate FAME cis and trans isomers with DB-23

Baseline resolution of FAME cis and trans isomers

Excellent resolution of 40 FAMES in under 30 minutes
Best column to column reproducibility for FAMES analysis
modulating cellular functions. Areas
of particular interest include the
cytotoxic actions of butyric acid and
sphingolipids and how dietary fatty
acids modify mitochondrial membrane structure. The laboratory uses
DB-23 columns and FID detectors to
determine the concentration of individual lipid metabolites from all
types of biological samples.

By Cameron George
Technical Support Engineer

Capillary columns for the analysis of

FAMES must possess the necessary
selectivity and efficiency for resolution and accurate quantification of
the fatty acids that make up complex
lipid classes. DB-23, (50%-Cyano-

At the University of California at

Davis, Professor J. Bruce German and
his laboratory are focused on understanding the role of dietary lipids in

O R D E R

I.D. (mm)

Length (m)

Film (m)

Part Number

0.25

60

0.25

122-2362

Oven:

6
13

12

8
5

Injector:

Detector:

G U I D E

DB-23

Part No.:
Carrier:

Baseline Resolution of C16 and

C18 cis-, trans- isomers

For the analysis of FAMEs, the

proven selectivity of DB-23 combined
with the tightest quality control specifications in the industry provide
superior results every time.

Phase/Description

Column:

FAMES Analysis

propyl)-methylpolysiloxane, has a
very strong dipole characteristic,
providing excellent resolution of C16
and C18 FAME isomers. This resolution is not achieved using other common stationary phases. Increasing
column length to 60 meters produces
the required number of theoretical
plates for complete resolution.
Finally, each DB-23 is processed and
tested to the exacting specifications
for retention index and efficiency.
This helps to assure that the next
DB-23 will be like the last one.
The combination of column and
phase dimensions shown below has
been used successfully in the laboratories of UC Davis and Lipomic
Technologies, Inc.

DB-23
60 m x 0.25 mm I.D., 0.25 m
122-2362
Hydrogen at 43 cm/sec
(constant pressure mode)
130C for 1.0 min
130-170C at 6.5C/min
170-215C at 2.75C/min
215C for 12 min
215-230C at 40C/min
230C for 3 min
Split, 270C
Split ratio 50:1
FID, 280C

1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.

10
14

1
11

19

2
13

21

15
17

20

16

22

18

23 24
2526

27

28

29 30

31 32

33 34

C6:0
C7:0
C8:0
C9:0
C10:0
C11:0
C12:0
BHT
C13:0
C14:0
C14:1n5
C15:0
C16:0
C16:1n7(trans)
C16:1n7(cis)
C17:0
C17:1
C18:0
C18:1n9(trans)
C18:1n9(cis)
C18:1n7

22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.

C18:2n6
C18:3n6
C18:3n3
C18:2(d9,11)
C18:2(d10,12)
C20:0
C20:1n9
C20:2n6
C20:3n6
C20:4n6
C20:3n3
C20:5n3
C22:0
C22:1n9
C22:2n6
C22:4n6
C22:5n3
C24:0
C22:6n3
C24:1n9

Short Analysis Time

35

37
37
38 3940

10

12

14

16
Time (min)

18

20

22

24

41

26

28

Chromatogram provided courtesy of Steve Watkins and Jeremy Ching, Lipomic Technologies, Inc.

www.agilent.com/chem

Separation Times Volume 14 Number 3 2001

13