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INTISARI

Bakteri Streptococcus alpha merupakan bakteri paling dominan


dan berperan penting pada awal pembentukan plak gigi. Pertumbuhan
bakteri Streptococcus alpha dapat dihambat dengan antibakteri bahan alam
yang mengandung flavonoid seperti ekstrak propolis Trigona sp. Tujuan
penelitian ini adalah untuk mengetahui pengaruh ekstrak propolis Trigona
sp dalam menghambat pertumbuhan bakteri Streptococcus alpha isolat
plak gigi anak.
Penelitian in vitro dilakukan pada ekstrak propolis Trigona sp
konsentrasi 4%, 6%, 8% dan 10% yang diperoleh dari hasil maserasi
sarang lebah Trigona Sp dari Ambarawa-Jawa Tengah dengan etanol 70%.
Isolat bakteri Streptococcus alpha diperoleh dari plak gigi pasien anak di
klinik kedokteran gigi anak RSGM Prof Soedomo. Zona hambatan
pertumbuhan bakteri Streptococcus alpha diamati dengan metode difusi.
Replikasi 3 kali tiap konsentrasi. Data dianalisis dengan uji Kruskal-Wallis
dan Man-Whitney.
Hasil penelitian menunjukkan terdapat perbedaan rata-rata zona
hambat pertumbuhan Streptococcus alpha yang bermakna (p<0,05) antara
ekstrak propolis Trigona sp konsentrasi 4%, 6%, 8% dan 10%. Pada
peningkatan konsentrasi ekstrak propolis Trigona sp tiap 2% terjadi
peningkatan zona hambat antara 0,38-0,57 mm. Terdapat perbedaan ratarata zona hambat bermakna antar tiap konsentrasi ekstrak propolis Trigona
sp (p 0,05). Disimpulkan bahwa pada konsentrasi 4% - 10% ekstrak
propolis Trigona Sp terjadi peningkatan rata-rata zona hambat
pertumbuhan bakteri Streptococcus alpha isolat plak gigi anak.

Kata kunci : Ekstrak propolis Trigona Sp, bakteri Streptococcus alpha,


plak gigi anak, zona hambatan pertumbuhan

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ABSTRACT

The Streptococcus alpha bacteria is the most dominant bacteria and plays
an important role in the early formation of dental plaque. Streptococcus alpha
bacteria growth can be inhibited by natural antibacterial ingredients such
as Trigona sp propolis extract containing flavonoids. The purpose of this study
was to determine the effect of Trigona sp propolis extract in inhibiting the growth
of Streptococcus alpha bacteria isolated from dental plaque of children.
In vitro studies conducted on Trigona sp propolis extract concentration
of 4%, 6%, 8% and 10% obtaine from the maceration of honeycomb AmbarawaTrigona Sp of Central Java with 70% ethanol. Streptococcus alpha bacteria
isolates obtained from dental plaque of pediatric patients in pediatric dentistry
clinic of Prof Soedomo dental hospital. Streptococcus alpha bacteria
growth inhibition zone was observed with diffusion method. Replication of
three times for each concentration. Data were analized with the Kruskal-Wallis
and Man-Witney test.
The results showed there were significant differences in the average
growth inhibition zone of Streptococcus alpha (p <0.05)between Trigona
sp propolis extract concentration of 4%, 6%, 8% and 10%. By increasing the
concentration of Trigona sp propolis extract occurs every 2% increase
in inhibition zone between 0.38 to 0.57 mm. There are significant differences
in the average inhibition zone between each concentration of Trigona
sp propolis extract (p 0.05). It was concluded that the concentration of 4% to
10% Trigona Sp propolis extact increased the average inhibition zone of
Streptococcus alpha bacteria growth of childrens dental plaque.

Keywords : Trigona Sp propolis extract, Streptococcus alpha bacteria, dental


plaque of children, growth inhibition zone

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