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DICKY BAGUS WIDHYATMOKO

18 19 MARET 2015
DEPARTEMEN MIKROBIOLOGI KLINIK, FAKULTAS KEDOKTERAN
UNIVERSITAS AIRLANGGA

BAKTERIOLOGI INFEKSI KULIT &


JARINGAN LUNAK

POKOK BAHASAN
Staphylococcus spp
Mycobacterium leprae

DISEDIAKAN
Staphylococcus :

Uji Katalase

S. aureus

Uji Koagulase

S. epidermidis

Mannitol Salt Agar


(MSA)

S. saphrophyticus

Novobiocin test
MRSA Identification
Mycobacterium leprae :
slide only

Identifikasi Staphylococcus
Uji Katalase
Uji Koagulase
Mannitol Salt Agar (MSA)
Novobiocin test

Staphylococcus spp

Use of Catalase Test


The

catalase test is primarily used to


distinguish among Gram-positive cocci
Staphylococcus are catalase-positive,

of the genera Streptococcus


Enterococcus are catalase-negative.

members

and

Catalase Test
Metode :
slide (drop) method
tube method
tube (slant) method

Catalase
2H2O2 O2 + 2H2O
Streptococci vs. Staphylococci

Catalase Test

Catalase Test

Catalase POS

Staphylococcus

Catalase NEG

Catalase Test (tube method)

Catalase Test (tube (slant)


method)

Coagulase Test
differentiates strains of Staphylococcus aureus

from S. epidermidis and other coagulasenegative species


S. aureus strains are usually capable of
coagulating EDTA-treated plasma in the tube
test and will produce clumps of cells in the
slide test.

Coagulase Test
method :
Slide Test
Tube Test

Coagulase Test (Slide Test)

Differential
Characteristics
S. aureus
Coagulase

Fibrinogen Fibrin

Staphylococcus aureus

Coagulase POS

Coagulase NEG

Mannitol Salt Agar


Contains :
Mannitol
Phenol Red as a pH indicator (pH = 7.8)
Reaction

Mannitol Fermented acid produced


pH < 6.9 : yellow color
pH > 8.4 : red color

various spesies on
Tripticase Soy Agar (TSA)

A. S. aureus

B. S. epidermidis

C. E. coli

various spesies on
Tripticase Soy Agar (TSA)

A. S. aureus

B. S. epidermidis

C. E. coli

Novobiocin Test
Differentiate between S. epidermidis w/ S. saprophyticus
Using Novobiocin disc ( 5 g)
< 15 mm = Resisten S. saprophyticus

Identifikasi MRSA
MRSA : Methicillin-Resistant Staphylococcus

aureus
Methicillin Oxacillin Cefoxitin
CLSI M100
Using Cefoxitin disc :
21 mm : MRSA
> 21 mm : non - MRSA

Suspect MRSA

Collect Sample from wound

Collect Sample from nose

Mycobacterium Leprae
Sample : Skin scrap using scalpel
Using Slide with Ziehl-Neelsen Stain method
Presence

of globi (singular = globus):


encapsulated amphophilic masses of nonviable bacilli found within macrophages or in
extracellular space

Result : (Bacterial Index)


1+ At least 1 bacillus in every 100 fields.
2+ At least 1 bacillus in every 10 fields.
3+ At least 1 bacillus in every field.
4+ At least 10 bacilli in every field.
5+ At least 100 bacilli in every field.
6+ At least 1000 bacilli in every field.

On Hand
Pengamatan & Pelaporan Preparat Demo
Uji Katalase
Kultur Media dari spesimen langsung (amies

swab) dengan :
Blood Agar Plate
McConkey Agar