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Arabidopsis Genetics Post-Lab Assignment

(10 points)
Name:_____Jeff Tong________

Lab Section___105______

Table 1: Phenotypic Analysis of Arabidopsis seedlings


Seedlin

Trichom

Hypocot

Seedlin

Trichom

Hypocot

Seedlin

Trichom

Hypocot

es

yl

es

yl

es

yl

No.

( + or - )

Length
(S, M or

No.

( + or - )

Length
(S, M or

No.

( + or - )

Length
(S, M or

T)

T)

21

22

23

6
7

T)

41

42

43

24

44

25

45

26

46

27

47

28

48

29

49

10

30

50

11

31

51

32

52

12
13

33

53

14

34

54

15

35

55

16

36

56

17

37

57

18

38

58

19

39

59

20

40

60

Table 2: X2 Analysis of Phenotypic Analysis of Arabidopsis seedlings


sample of ___45__ seedlings
+

trichomes trichome

short

medium

tall

hypocotyls

hypocotyls

hypocotyls

s
observed (o) 30

15

21

17

expected (e)

11

11

23

11

10

-6

-4

16

16

100

36

16

0.4706

1.4545

9.0909

1.5652

1.4545

34

deviation (o- -4
e)
(o-e)2
(o-e)2/e
X2=((o-e)2/

X2=1.9251

X2=12.1106

e)

Table 3: Hypothesis Testing of Phenotypic Analysis of Arabidopsis seedlings


Degrees of

Critical Value

Freedom
trichome 1

Reject Null
Hypothesis?

3.84

No

5.99

Yes

character
hypocotyl 2
length
character

Table 4: Expected (Predicted) Phenotypic Ratios for the Dihybrid Cross


Phenotype

Expected Ratio (show your math)

short hypocotyl + trichomes

(3/16)*45 = 8

short hypocotyl - trichomes

(1/16)*45 = 3

medium hypocotyl + trichomes

(6/16)*45 = 17

medium hypocotyl - trichomes

(2/16)*45 = 6

tall hypocotyl + trichomes

(3/16)*45 = 8

tall hypocotyl - trichomes

(1/16) *45 = 3

Table 5: X2 Analysis of the Dihybrid Cross


sample of ___45___ seedlings
short
+

short
-

trichomes trichome

medium
+

medium
-

tall
+

tall
-

trichomes

trichomes

trichomes

trichomes

s
observed (o) 14

11

expected (e) 8

17

-6

-2

-2

(o-e)2

36

16

36

(o-e)2/e

4.5

5.3333

2.1176

0.5

1.3333

deviation
(o-e)

X2=((o-e)2/

X2=13.7842

e)

Table 6: Hypothesis Testing of Dihybrid Cross

Degrees of

Critical Value

Freedom
Sum of all

Reject Null
Hypothesis?

11.07

Yes

characters

Part II Questions (page 5-25):


14. Used the pooled data from the Canvas site for the following calculations:
a. What percentage of the plants with a CC genotype had the expected
phenotype?
54.17%
b. What percentage of the plants with a Cc genotype had the expected
phenotype?
45.45%
c. What percentage of the plants with a cc genotype had the expected
phenotype?
68.42%

15. Why do you think the percentages that you calculated in #14 above are less
than 100%?
Due to the subjectivity of classifying a hypocotyl as short, medium, or tall, the
results will vary depending on who is classifying, and even what angle it was
viewed at (some plants grew horizontally or diagonally, which may have had an
effect on their perceived or actual length). In addition, as similarly seen in human
height, length of a hypocotyl may depend on environmental factors, and beyond

this, length may be represented on a continuous spectrum instead of purely


segregated into three distinct categories of short medium and tall.