Fresh leaves
Homogenize for 5 Min. in
MeOH H2O (4:1) (10 X vol.or Wt) filter
Residue
Filtrate
Extract with
EtOAc (x5), filter
Residue
Filtrate,
CHCl3 extract
Evaporate
Fabre (Mainly
Polysaccharide)
Dry, evaporate
Neutral Extract
(Fat, Waxes)
CHCl3-MeOH extract
Dry, evaporate
Basic extract
(Most Alkaloids)
Basify to pH 10
with
NaOH,
extract
with
CHCl3-MeOH
(3:1, twice) and
CHCl3
MeOH extract is
polar extract (quaternary
Alkaloids and N-Oxides)
Fig. A general Procedure for extracting plant tissues and fractionating into different classes according to
polarity (Harborne, 1983).