Chromatography is defined
by
components
Adsorption
of
mixture
by
adsorption
and
is
defined
solubility.
as
the
stationary
mobile
non-moving
phase
in
chromatography,
through
which
The
phase
mobile
and
phase
carries
the
mixture
dissolves
in
the
stationary phase.
phase.
polarity
instance,
of
a
Solubility and
substances.
separation
of
components
affinities
of
the
different
through
For
column
2
column being composed of a polar
Following
like
eluates
the
dissolves
principle
like,
of
the
grinded
sample.
The
pure
Pasteur pipette.
principle
as
column
in
which
the
phase
is
non-polar,
the
B. Column Chromatography
Figure 2 shows the column
chromatography
column
to
experiment
The
experiment
objective
is
to
of
separate
be
was
set-up.
utilized
The
in
the
prepared
by
this
the
Pasteur
pipette,
and
filling
the
layer
chromatography,
and
to
3
one of the lines. The pure extract
and each of the colored eluate
collected were spotted on the thin
layer
chromatography
times.
Then,
the
plate
10
developing
(1:1).
The
beaker
was
chromatography set-up
DCM-hexane,
solvent,
was
the
run
extracting
through
the
was
introduced
to
the
Through
the
same
minutes
to
allow
the
solvent
system to equilibrate.
After 15 minutes, the TLC plate
was placed inside the chamber. The
chamber was once again covered
with a watch glass, and the solvent
system was left to develop. After
reaching the 1.5 cm mark, the TLC
plate
was
removed
from
the
chamber to dry.
another
eluate
batch
of
colored
Figure 3: Development
After
drying,
the
distances
4
with a pencil for the measurement
of
the
distance
that
they had
travelled.
is
Rf =
the
most
inferred
component
non-polar
chromatography.
Based
is
the
orange
intermediate
in
Volume of
component
Yellow
Orange
Red orange
that
formula:
in
Chromatography
column
on
the
The
color
of
eluates
collected
being Capsorbin.
from
the
column
5
Figure 4 shows the TLC plate
the
experiment
yielded
correct
have
component
red
orange
eluates
development
shown
that
the
had
the
yellow
highest
during
the
and
the
stage
computed Rf values.
Color of
Distance
compone
of
nt
componen
Rf value
Chromatography is a method
origin (X)
in cm
4.4 cm
0 cm
0 cm
0.7 cm
were
some
errors
distances
absorption.
In
column
There
obtained.
solvent
Conclusion
orange
the
the
t from
Red
Yellow
Orange
Red
since
expected
Ideally,
were
none
travelled
of
by
not
the
the
used
with
solvent.
the
least
Among
non-polar
the
three
affinity
to
the
polar
volume
collected.
The
of
the
thin
eluate
layer
6
chromatography (TLC) experiment
t-prep/mcat/chemical-p
performed
did
expected
results.
not
yield
the
rocesses/separations-
Had
the
purifications/a/principles-of-
the
have
yellow
had
component
the
highest
the
components.
It
affinity
to
the
polar
stationary phase.
18,
2016
Thin
18,
2016
from
http://www.chemguide.co.uk/analy
sis/chromatography/thinlayer.html
Boston College. (n.d.).
References
of
was
chromatography
October
18,
2016
Retrieved
from
http://www.bc.edu/schools/cas/ch
from
emistry/academics/undergrad/org/
https://www.khanacademy.org/tes
fall/TLC.pdf