v.

max patr
v.max sln

0
0.2
0.4
0.6
0.8
1
1.2

1/0,0134= 74,62
1/0,0087=114,94

KM patron= 74,62x3,4786 = 259,57

KMSln= 114,94x 0,1208= 13,88

Tiempo
15
15
15
15
15
15
15
15

[AR]

Absorbancia

0
0.0334
0.043
0.0754
0.097
0.122
0.161
0.185

0
0.103
0.137
0.249
0.324
0.41
0.548
0.632

0
0.048
0.114
0.194
0.267
0.341
0.403

V=[AR]/T

Absorbancia
f(x) = 0.3478571429x - 0.0134285714
R = 0.9968057698

[S]
0
0.44
0.88
1.77
2.66
3.55
4.44
5.33

0.0022266667
0.0028666667
0.0050266667
0.0064666667
0.0081333333
0.0107333333
0.0123333333

V
0.0028666667
0.0050266667
0.0064666667
0.0081333333
0.0107333333
0.0123333333

1/V

V/[S]
0
0.00325758
0.00283992
0.00243108
0.00229108
0.00241742
0.00231395

1/[S]
0

348.837209
198.938992
154.639175
122.95082
93.1677019
81.0810811

1.13
0.56
0.376
0.282
0.225
0.1876

Absorbancia
f(x) = 3.4435604349x - 0.008168015
R = 0.9996609847

Velocidad en funcin de [S]

f(x) = - 0.0896193739x + 0.0032723467

R = 0.6923645652

orbancia

n de [S]

1/V vs 1/[S]
f(x) = 0.0033016117x - 0.0771057912
R = 0.9815291359

1/V vs 1/[S]
0.0771057912

[S]

V
0.22 0.00222667
0.44 0.00286667
0.88 0.00502667
1.33 0.00646667
1.77 0.00813333
2.22 0.01073333
2.66 0.01233333

1/s
1/v
4.54545455 449.101796
2.27272727 348.837209
1.13636364 198.938992
0.7518797 154.639175
0.56497175 122.95082
0.45045045 93.1677019
0.37593985 81.0810811

line-Burk
f(x) = 89.1578939295x + 78.3456192936
R = 0.9329224792

v
0.00222667
0.00286667
0.00502667
0.00646667
0.00813333
0.01073333
0.01233333

ho

michaelis -menten

line-Burk

939295x + 78.3456192936
4792

f(x) = - 0.3881565342x + 0
R = 0.555274281

v/[S]
0.01012121
0.00651515
0.00571212
0.00486216
0.0045951
0.00483483
0.00463659

hoftee]

f(x) = - 0.3881565342x + 0.0085465539

R = 0.555274281

[AR]
0
0.0334
0.043
0.0754
0.097
0.122
0.161
0.185

[S]

V/[S]

V=[AR]/T
1 0.00286667
1.5 0.00502667
2 0.00646667
2.5 0.00813333
3 0.01073333
3.5 0.01233333

V=[AR]/T

Absorbancia

0
0.103
0.137
0.249
0.324
0.41
0.548
0.632

V=[AR]/T

[S]

0
0.00222667
0.00286667
0.00502667
0.00646667
0.00813333
0.01073333
0.01233333

1/v
0
0.5
1
1.5
2
2.5
3
3.5

449.101796
348.837209
198.938992
154.639175
122.95082
93.1677019
81.0810811

Michaelis-Menten
V vs [S]

Chart Title

f(x) = 1.6121955476x + 0.0010844783

R = 0.2425626519

0
0
0.00445333 0.00222667

0.00286667
0.00335111
0.00323333
0.00325333
0.00357778
0.00352381

0.00286667
0.00502667
0.00646667
0.00813333
0.01073333
0.01233333

1/[S]
2
1
0.66666667
0.5
0.4
0.33333333
0.28571429

ten

V/[S]
0
0.00445333
0.00286667
0.00335111
0.00323333
0.00325333
0.00357778
0.00352381

1/[S]

Chart Title

0844783

Lineawer
1/Vo vs 1

1/[V]
0

0.66666667
0.5
0.4
0.33333333
0.28571429

198.938992
154.639175
122.95082
93.1677019
81.0810811

f(x) = 303.9434926404x - 2.2593106662

R = 0.9960672932

Lineawer-Burk
1/Vo vs 1/[S]

34926404x - 2.2593106662
72932

Calibracin

concentracion Absorbancia
0
0
0.2
0.048
0.4
0.114
0.6
0.194
0.8
0.267
1
0.341
1.2
0.403

[AR]

Absorbancia

0
0.334
0.43
0.754
0.97
1.22
1.61
1.85

0
0.103
0.137
0.249
0.324
0.41
0.548
0.632

[S]

V=[AR]/T
0
0.5
1
1.5
2
2.5
3
3.5

V/[S]

V=[AR]/T

0.04453333 0.02226667

0
0.02226667
0.02866667
0.05026667
0.06466667
0.08133333
0.10733333
0.12333333

f(x) = 0.3478571429x - 0.0134285714

R = 0.9968057698

V=[AR]/T
0
0.02226667
0.02866667
0.05026667
0.06466667
0.08133333
0.10733333
0.12333333

[S]

1/v
0
0.5
1
1.5
2
2.5
3
3.5

44.9101796
34.8837209
19.8938992
15.4639175
12.295082
9.31677019
8.10810811

Michaelis-Menten
Vo vs [S]

f(x) = - 1.2166790347x + 0.1011364151

R = 0.0414180902
Chart

Title

f(x) = - 1.2166790347x + 0.1011364151

R = 0.0414180902
Chart

0.02866667
0.03351111
0.03233333
0.03253333
0.03577778

0.02866667
0.05026667
0.06466667
0.08133333
0.10733333

Title

Calibracin

0.0134285714

1/[S]
2
1
0.66666667
0.5
0.4
0.33333333
0.28571429

V/[S]
0
0.04453333
0.02866667
0.03351111
0.03233333
0.03253333
0.03577778
0.0352381

Michaelis-Menten
Vo vs [S]

1/[S]

1
0.66666667
0.5
0.4
0.33333333
0.28571429

1364151

Chart Title

1/v

34.8837209
19.8938992
15.4639175
12.295082
9.31677019
8.10810811

1364151

Chart Title

f(x) = 36.9490082571x - 2.9579142543

R = 0.9909078367
Lineawever-Burk

1/Vo vs 1/[S]