BS
3-4
BIOCHEMISTRY
Isotonic Solutions
Isotonic solution is a solution in which the concentration of solutes is
equal,
so:
a. water diffuses into and out of the cell at equal rates.
b. theres no net movement of water across the plasma membrane
c. the cells retain their normal shape.
If the concentration of solute (salt) is equal on both sides, the water will
move back in forth but it wont have any result on the overall amount of water on
either side.
Animal cell and plant cell will retain its normal shape and size.
Hypotonic Solution
Hypertonic refers to the solution in a concentration gradient having the
greater concentration (of solute). Hypo means less.
In plant cells, the central vacuoles will fill and the plant becomes stiff and
rigid, the cell wall keeps the plant from bursting.
In animal cells, the cell may be in danger of bursting , organelles called
contractile vacuoles will pump water out of the cell to prevent this.
Experiment no. 1
BIOLOGICAL MEMBRANES
(Dialysis, Osmosis, Diffusion)
SAN MIGUEL, Djanelle Mei M.
Schematic
BIOCHEMISTRY
PSYCHOLOGY 3-4
1
Diagram
BS
DIFFUSION
1.1 Diffusion of solutes in water
Prepare a series
of four test
tubes
Repeat the
determination.
Melt
gelatine/agar
according to
manufacturers
procedures.
Dispose the
used materials
and clean your
workplace.
2. Osmosis
2.1 Osmosis (Longanisa Casing Model)
Prepare
two set
ups.
Place ~10 mL of
saturated sugar
solution in a test
tube.
Record gathered
data.
Use an Iron
clamp to
fasten the
test tube.
Tabulate the
results.
Prepare 3
clean glass
slides and
cover slips.
Use a
STERILE
lancet to
make a
puncture at
the donors
Dispose the
used materials
property and
clean your
workplace.
Record the
observation.
Compare the sizes of
the RBCs of the
different slides.
3. Dialysis
Prepare two set ups. (3 test
tubes for each set up.)
Prepare Methylene Blue,
Chloride and Calcium.
Prepare 5 Beakers and
Use an Iron
clamp to
fasten the
test tube.
Does a faint
blue color in
the beakerwater
yes
Record the
observation.
No