MTh 7-10AM
Rohani B. Cena, DVM, MSc
Rm108 Institute of Molecular Biology and
Biotechnology, National Institutes of Health, UP
Manila
MWTh 8-10AM
MICROSCOPY
Types of Microscope
Simple Light Microscope (Uses single lens)
Compound Microscope (Uses a set of lenses)
Stereoscopic/Dissecting Microscope
Fluorescence Microscope
Phase-contrast Microscope
Differential Interference Microscope
Electron Microscope(Uses electron beams on electromagnetic lenses)
Scanning Electron Microscope (SEM)
Transmission Electron Microscope(TEM)
Stereoscopic/Dissecting Microscope
Sample View
Fluorescence Microscope
Sample View
Sample View
Sample View
Sample View
Sample View
Mechanical Parts
-
Base
-Nosepiece
Pillar
-Dust shield
Inclination Joint
Arm
Stage
Draw tube
Optical Parts
Terms to Know
Magnifying Power
10x, 40x, 100x
-ability to magnify the
view of an object
Numerical Aperture
0.30, 0.65, 1.30
- Light refracting capability
Resolving Power
-ability to reveal adjacent details as separate and distinct.
-typical compound microscope has a resolving power of 0.2-0.4 nm
Working Distance
- distance between the lens of the objective and the object on
the slide when it is in focus.
Old
New
Objectives
Adjustment
Knobs
Illumination
(Light Source)
Stage Clips
Separate
Joined
Ambient Light
using Mirror
Immovable
Light Bulb
Movable
Compound
Stereoscope
Magnification
10x-100x
4x
Orientation
Separate
Joined
Movement of object
across field
Inverted
Same
Type of Image
Produced
Virtual
Real
Formulas
Magnifying Power
of Ocular Lens
Example:
Magnifying Power of HPO = 40x
Magnifying power of Ocular =10x
40 x 10= 400x
Example:
Number of Lines in stage = 5
Number of Lines in ocular = 9
Ocular
Stage
Example:
Size of Drawing = 3 cm or 30mm
Actual size of object = 0.0648 mm
30mm/0.0648mm = 462.96x
or 463x
3cm