2

FAAH

Oleamide

NH2

O-phthalaldehyde

Oleic acid

Na2 SO3

1-sulphonatoisoindole

OH

6kb
3kb
1kb

150

150

100
a c t iv it y

c o n tro l)

F A A H -2

50

(%

c o n tro l)

50

(%

F A A H

a c t iv it y

100

0
-9

-8

-7

-6

-5

-1 0

-4

-9

-8

-7

-6

-5

[U R B 5 9 7 ] L o g [M ]

[U R B 5 9 7 ] L o g [M ]
-5 0

-5 0

(a )

(b )

FA A H

p r o t e in )
( n m o l/ m in / m g

a c t iv it y

15

A rD A h y d ro ly sis

0
-7

-6

-5

40

60

80

100

50

0
-1 0

-4

[U R B 5 9 7 ] L o g [M ]

-8

-6

-4

[U R B 5 9 7 ] L o g [M ]
-5 0

20

(b )
p r o t e in )

(a )

a c t iv it y

-5 0

F A A H

-8

20

A rD A ( M )

( n m o l/ m in / m g

-9

c o n t r o l)

a c t iv it y

(%

F A A H -2

50

100

(%

100
A c t iv it y

+ 50 nM URB597

S y D A h y d ro ly sis

c o n t r o l)

C o n tro l (- in h ib ito r )

150

150

F A A H

10

15

C o n tro l (- in h ib ito r)

10

+ 50 nM URB597
5

0
0

100

200

300

O D A ( M )

400

500