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Sex Transm Inf 2001;77:271275
Original
article
271
Objective: To assess the association between vaginal douching and sexually transmitted
infections (STI) among a group of female sex workers (FSWs) in Nairobi, Kenya.
Methods: This study was part of a randomised, placebo controlled trial of monthly prophylaxis
with 1 g of azithromycin to prevent STIs and HIV infection in a cohort of Nairobi FSWs. Consenting women were administered a questionnaire and screened for STIs.
Results: The seroprevalence of HIV-1 among 543 FSWs screened was 30%. HIV infection was
significantly associated with bacterial vaginosis (BV), trichomoniasis, gonorrhoea, and the presence of a genital ulcer. Regular douching was reported by 72% of the women, of whom the
majority inserted fluids in the vagina, generally after each sexual intercourse. Water with soap was
the fluid most often used (81%), followed by salty water (18%), water alone (9%), and a
commercial antiseptic (5%). Douching in general and douching with soap and water were significantly associated with bacterial vaginosis (p = 0.05 and p = 0.04 respectively). There was a significant trend for increased frequency of douching and higher prevalence of BV. There was no
direct relation observed between douching and risk for HIV infection or other STIs.
Conclusion: The widespread habit of douching among African female sex workers was
confirmed. The association between vaginal douching and BV is of concern, given the increased
risk of HIV infection with BV, which has now been shown in several studies. It is unclear why we
could not demonstrate a direct association between douching and HIV infection. Further
research is required to better understand the complex relation between douching, risk for bacterial vaginosis, and risk for HIV and other STIs.
(Sex Transm Inf 2001;77:271275)
International Centre
for Reproductive
Health, Department of
Obstetrics and
Gynaecology, Ghent
University, Ghent,
Belgium
K Fonck
M Temmerman
Department of
Medical Microbiology,
University of Nairobi,
Nairobi, Kenya
K Fonck
R Kaul
F Keli
J J Bwayo
Department of
Community Health,
University of Nairobi,
Nairobi, Kenya
E N Ngugi
Departments of
Medical Microbiology,
Community Health
Sciences and
Medicine, University
of Manitoba,
Winnipeg, Canada
S Moses
Correspondence to:
Professor Dr M
Temmerman, ICRH Ghent
University, De Pintelaan 185,
9000 Gent, Belgium
marleen.temmerman@
rug.ac.be
Accepted for publication
18 April 2000
Introduction
Heterosexual intercourse is the major route of
transmission of HIV in sub-Saharan Africa.
The role of ulcerative and non-ulcerative sexually transmitted infections (STIs) in facilitating
the transmission of HIV is well established.
However, the role of local genital tract factors
in HIV transmission is less clear.
There is some evidence that frequent
vaginal douching may increase womens susceptibility to sexually transmitted agents,
through modification of the vaginal flora. Several studies have suggested that vaginal
douching may increase risk for cervical
infections.14 Others have shown that douching
predisposes women to pelvic inflammatory
disease (PID),58 with a twofold increased risk
of PID associated with douching.6 8 Genital
tract infections such as cervicitis and PID
have, in turn, been identified as a risk factor for
HIV acquisition. Hence, vaginal douching
may indirectly have a facilitating role in the
heterosexual transmission of HIV. In addition,
vaginal douching has been reported to be
associated with reduced fertility.9 Finally,
douching has been suggested as a risk factor
for cervical cancer, although the evidence for
this has been inconsistent.10
The insertion of various substances into the
vagina is a common global practice. Intravaginal substances, largely through their astringent
www.sextransinf.com
HIV negative
(n = 373)
HIV positive
(n = 161)
30.4
10.6
30.9
9.9
50%
50%
16.3
6.1
3.9
13.9
118
46%
54%
15.6
6.2
4.0
19.1
93
34 %
47%
19 %
57 %
31 %
23 %
15 %
55 %
28 %
72 %
35 %
55%
10 %
47 %
31 %
26 %
16 %
66 %
27 %
73 %
OR
(95% CI)
p Value
0.4
0.4
0.2
0.9 (0.71.1)
0.005
0.8
0.5
0.08
0.02
1.9 (1.03.6)
2.1 (1.23.9)
1.0 (reference)
0. 8 (0.61.0)
1.0 (0.81.3)
1.1 (0.81.5)
1.1 (0.81.5)
0.7 (0.50.9)
1.0 (0.81.4)
1.0 (0.71.6)
0.04
0.01
NA
0.02
0.5
0.2
0.4
0.01
0.5
0.9
Bacterial vaginosis
Candida
Trichomoniasis
Chlamydia
Gonorrhoea
Syphilis
Clinical ulcer
CIN
HIV negative
HIV positive
No (%)
No (%)
OR (95% CI)
p Value
138/303 (46)
28/303 (9)
43/369 (12)
30/327 (9)
30/370 (8)
23/369 (6)
2/373 (1)
4/373 (1)
73/129 (57)
11/130 (9)
41/156 (26)
11/124 (9)
22/156 (14)
8/159 (5)
5/161 (3)
5/161 (3)
1.5 (1.02.3)
0.9 (0.41.9)
2.7 (1.74.4)
1.0 (0.52.0)
1.9 (1.03.3)
0.8 (0.31.8)
6.0 (1.131.0)
3.0 (0.811.2)
0.05
0.9
<0.001
1.0
0.04
0.7
0.03
0.2
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STI and vaginal douching in a population of female sex workers in Nairobi, Kenya
Table 3
Bacterial vaginosis
Vaginal yeast
Trichomonas vaginalis
Neisseria gonorrhoeae
Chlamydia trachomatis
Syphilis
CIN
HIV
Never
douched
Regular douching,
any product
Ever douche
with water alone
Ever douche
with soap
Ever douche
with salt
No (%)
No (%)
No (%)
No (%)
No (%)
50/121 (41)
9/120 (8)
22/147 (15)
15/147 (10)
10/134 (8)
9/147 (6)
2/150 (1)
44/148 (30)
161/310 (52)*
30/313 (10)
64/381 (17)
37/382 (10)
31/318 (10)
22/381 (6)
7/392 (2)
117/385 (30)
13/26 (50)
1/27 (4)
2/30 (7)
1/30 (3)
1/27 (4)
1/30 (3)
1/31 (3)
3/30 (10)*
125/234 (53)*
23/236 (10)
53/286 (19)
28/287 (10)
27/235 (11)
20/285 (7)
5/293 (2)
92/289 (32)
28/50 (56)**
3/51 (6)
10/62 (16)
6/62 (10)
3/53 (6)
2/63 (3)
2/64 (3)
18/62 (29)
BV negative
(n = 220)
BV positive
(n = 211)
31.0
49%
16.0
6.4
3.8
13.8
110
30.5
45%
15.9
6.2
4.0
14.9
116
35%
49%
16%
57%
26%
23%
22%
14%
53%
6%
31%
3%
19%
22%
10%
5%
26%
34%
45%
18%
56%
32%
25%
26%
16%
63%
2%
29%
3%
12%
23%
21%
13%
35%
OR (95% CI)
0.9 (0.81.1)
0.9 (0.51.5)
0.9 (0.51.5)
1.0 (reference)
1.0 (0.71.5)
1.3 (0.92.0)
1.1 (0.71.7)
1.1 (0.91.4)
1.1 (0.81.4)
1.5 (1.02.2)
0.4 (0.11.0)
0.9 (0.61.4)
1.1 (0.71.8)
0.7 (0.51.0)
1.0 (0.81.3)
2.3 (1.34.1)
3.0 (1.46.5)
1.5 (1.02.3)
p Value
0.5
0.4
0.7
0.8
0.2
0.3
0.5
0.7
0.7
NA
0.8
0.2
0.7
0.5
0.6
0.04
0.06
0.9
0.8
0.06
0.9
0.003
0.003
0.05
www.sextransinf.com
Discussion
The current study confirms that vaginal
douching is a widespread practice among
African female sex workers, as previously
described. In our study, 72% of the women
reported vaginal douching. Vaginal douching
is also widely practised among pregnant
women and women attending STD clinics in
Africa.13 16 The use of vaginal products for the
treatment of vaginal symptoms as well as the
use of vaginal agents to achieve a tightening
eVect is widespread.13 17 18 The specific preparations used for douching vary according to
local cultural factors. In several African
settings, herbs and dry leaves are used for
treatment of vaginal infections or for a tightening eVect to achieve dry sex.12 19 Although
the question was not specifically asked in our
study, it appears that personal hygiene was the
main reason for douching, as most women did
so after each intercourse. Most women used
water alone or a combination of water and
soap. Commercial products were rarely used.
The women in our study belonged to a low
socioeconomic group, and hence may not have
been able to aVord rather expensive commercial products.
The STD and HIV rates in this population
are similar to other African cohorts, except for
the CIN rate of 2%, which is lower than
reported from most other studies, even in low
risk groups in east Africa.20 Health seeking
behaviour and previous screening and treatment of cervical lesions might explain these low
rates of CIN.
We describe a significant association between douching (with soap and water) and an
increased prevalence of bacterial vaginosis.
More frequent douching was also significantly
associated with more prevalent bacterial vaginosis. This is consistent with results from other
studies. A case-control study has shown that
genital hygiene accounts for a twofold increase
in the risk of bacterial vaginosis.21 Although the
cross sectional nature of these studies
precludes the establishment of a cause and
eVect relation (for instance, women with BV
symptoms may douche more often), the
finding in a prospective cohort study that
acquisition of bacterial vaginosis is associated
with douching22 makes a causal relation more
likely.
There is now considerable evidence that the
presence of bacterial vaginosis has a role in the
acquisition of HIV.23 Hence, vaginal douching
may indirectly facilitate the heterosexual
transmission of HIV infection. We did not find
a direct association between douching and
HIV prevalence, but other factors associated
with douching in our cohort may have acted to
reduce HIV risk. Women who reported
douching were more likely to report using
condoms all the time. Vaginal douching
appeared to be used as a form of personal
hygiene, and it seems reasonable that women
more concerned with hygiene might also be
more likely to use condoms. The association
between douching and BV would still be
apparent, but this increased condom use
would bias our ability to detect any eVect of
BV on enhanced HIV acquisition. As has been
seen in previous studies on this subject, there
appears to be a complex relation between HIV
infection and vaginal douching. In a study in
the Central African Republic, Gresenguet et al
found a positive association between HIV and
the use of vaginal agents for the self treatment
of discharge and itching.12 In women without
vaginal symptoms, however, the use of vaginal
agents was not associated with HIV infection.
Dallabeta et al found an increased prevalence
of HIV infection among women using
non-commercial medicines in Malawi.13 The
potential mechanisms whereby vaginal
douching could possibly enhance HIV
transmission are twofold: (1) through
irritation of the vaginal mucosa, thus promoting the proliferation of lymphocytes which are
target cells for HIV24; and (2) through
dehydration of the vaginal mucosa, rendering
the vaginal epithelium more vulnerable to
local trauma.25
In summary, frequent douching seems to be
a common practice among female sex workers
in Africa, and we found a significant association between douching and bacterial vaginosis, but not with other STIs or HIV infection.
The association between vaginal douching and
bacterial vaginosis is of concern, given the
increased risk of HIV infection with BV, which
has now been shown in several studies. It is
www.sextransinf.com
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doi: 10.1136/sti.77.4.271
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