Jurnal 1

1. Judul penelitian
Uji Aktivitas Penurun Kadar Glukosa Darah Ekstrak Daun Eugenia polyantha
pada Mencit yang Diinduksi Aloksan
2. Nama metode penelitian
Metode uji pada hewan diabetes diinduksi diabetogen (in vivo)
3. Prinsip metode penilitian
Induksi diabetes dilakukan terhadap hewan uji dengan diabetogen seperti aloksan dll,
dimana perkembangan hiperglikemia diperiksa setiap hari. Pemberian obat antidiabetik
secara oral terhadap hewan uji diabetes dapat menurunkan kadar glukosa darah hewan uji
tersebut dibandingkan kontrol.
4. Induktor sakit
Aloksan
5. Parameter uji
Kadar glukosa darah
6. Parameter keberhasilan uji
Penurunan kadar glukosa darah puasa pada 2 jam setelah pemberian obat
7. Analisis data
Data yang diperoleh dianalisis dengan uji ANOVA pada tingkat kepercayaan 95%.
Apabila hasil uji ANOVA menunjukkan adanya perbedaan bermakna, maka dilanjutkan
dengan uji LSD untuk mengetahui kelompok mana saja yang mempunyai perbedaan
bermakna.

Bagan Kerja
Pembuatan Sediaan Uji

Langkah Kerja Pegujian Aktivitas Penurun Kadar Glukosa Darah Ekstrak Daun Eugenia
polyantha pada Mencit yang Diinduksi Aloksan

Kesimpulan
Dari hasil penelitian dan analisis data yang telah dilakukan, dapat ditarik kesimpulan bahwa
ekstrak etanol daun Eugenia polyantha dengan dosis 2,62 mg/20 g BB dan 5,24 mg/20 g
BB dapat menurunkan secara bermakna kadar glukosa darah mencit jantan yang diinduksi
dengan aloksan (p<0,05). Karena sampai saat ini masih belum diketahui kandungan daun

salam yang berkhasiat menurunkan kadar glukosa darah mencit, maka disarankan untuk
melakukan penelitian isolasi kandungan Eugenia polyantha dan menguji khasiat
hipoglikemianya.

Jurnal 2
1. Judul penelitian
Antimicrobial activity and composition profile of grape (Vitis vinifera) pomace extracts
obtained by supercritical fluids

2. Nama metode penelitian

Agar diffusion method
3. Prinsip metode penilitian
Mengukur diameter zona bening yang ada disekitar sampel dalam media agar, kemudian
dihitung konsentrasi hambat minimalnya.
The extracts with positive inhibition zone (9 mm or more) in the ADM assays, against at
least one microorganism, were submitted to the determination of the minimum inhibition
concentration (MIC) by microdilution method in culture broth.
4. Bakteri yang digunakan dalam uji antimikroba
The antimicrobial activity of grape pomace extracts obtained by different extraction
methods was evaluated against Gram-positive bacteria S. aureus, ATCC 25923
(American Type Culture Collection) and B. cereus ATCC 11778, Gram-negative bacteria
E. coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 and fungi strains C.
albicans ATCC 14053, Candida parapsilosis ATCC 22019, Candida krusei ATCC 6258
5. Parameter uji
Pengamatan zona disekitar sampel pada media agar
6. Parameter keberhasilan uji
Adanya zona bening dengan diameter > 9 mm disekitar sampel uji pada media agar
7. Analisis data
The results of the extraction yield (X0) were statistically evaluated by a one-way analysis
of variance (ANOVA) by means of the software Statistica for Windows 7.0 (Statsoft Inc.,
USA) in order to detect significant differences among the values. The significant
differences (p < 0.05) were analyzed by Tukey test
sBagan kerja

Buah Grape
Pomace

Sample
preparation

Extraction
sampel

Uji Kualitatif
dan Kuantitatif
Fenol

Soxhlet
extraction
(SOX)

Antimicrobial
activity

Phenolic compounds in the grape

pomace extracts were detected by
reversed-phase HPLC

Supercritical fluid
extraction (SFE)

Solventsolute
separation in rotary

Antimicrobial activity
Agar diffusion method
(ADM)

All cultures
Mikroorganisme dibiakkan
were
dalam Mueller-Hinton agar and
incubated in
broth media
aerobic
condition

diluted in fresh medium to achieve a final

concentration of approximately 107 or 108
CFU/mL

Then the agar surface was perforated with 7

mm diameter holes (four holes) with
aseptically cut

filled the hole with the different grape pomace

extracts, used at concentration of 20 mg
extract/mL in a water solution of DMSO

incubated at 36C for 24

h

examined the inhibition

effect

Keterangan :

A positive result was defined as an inhibition zone (halo size) of 9 mm or more

around the holes, indicating the presence of antimicrobial substance, or group of

substances, in the extracts tested.

The sterility control was prepared with the culture broth and the DMSO (without

grape pomace and microorganism suspension).

The growth control was prepared with the culture broth, the DMSO and 5 L inoculum

of the microbial suspension (107 CFU/mL) (without extracts).

All experiments were performed in duplicate and the plates incubated for 24 h at 36C
The extracts with positive inhibition zone (9 mm or more) in the ADM assays, against
at least one microorganism, were submitted to the determination of the minimum
inhibition concentration (MIC) by microdilution method in culture broth, as describe
by Michielin et al. (2009).

Kesimpulan
The supercritical fluid extracts presented the highest antimicrobial effectiveness compared to
the other grape pomace extracts due to the presence of antimicrobial active compounds.
Syrah extracts were less efficient against the microorganisms tested and Merlot extracts were
more active against Gram-positive bacteria.