External PCR Plate

PI Name:_______________________________________________

Date:__________________________

Name:_________________________________________________

Account:_______________________

Template:

Phone:________________________

Default*

Sybr (Default with Melt curve)

Email

Other (Modified):______________________

Comments:
*Default probe-based protocol: 1) 50 C for 2 min 2) 95 C for 10 min 3) 40 cylces of 95 C for 15s and 60 C for 1 min

gDNA/cDNA

RNA

Please write PI, researchers name, and date on the side of a plate
Please mark out the wells in use and label different detectors (if applicable)
1

10

11

12

13

14

15

16

17

18

(+)

(-)

21

22

23

24

A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P

Save Plate
Plates will be discarded after one week

SDS result files are now located on our website:

http://www.vetmed.ucdavis.edu/vme/taqmanservice/PI's last name.html

Thank you for using Real-time PCR Research and Diagnostics Core Facility
Your account will be charged $22.00 per run

Note: Please clearly type (in DIFFERENT COLORED BOLD font) or hand write modified protocols next to other.
Plates with slightly modified protocols, must have the change highlighted or circled.
Reaction volumes that are different than 12uL must be marked in the comments section.

qPCR samples needed for 280 wells 2.26.16

Sample

original ng/ul adjusted ng/ul final volume per well

23.11
17
0.24
18.52
14
0.29
23.68
18
0.22
10.31
8
0.50
2.81
2
2.00
15.94
12
0.33
23.18
17
0.24
41.28
31
0.13
22.86
17
0.24
9.00
7
0.57
5.76
4
1.00
35.32
27
0.15
9.98
7.5
0.53
9.45
7
0.57
8.88
7
0.57
-5.80
-4
-1.00
-6.85
-5
-0.80
2.83
2
2.00
(+)
125.00
12.5
0.32
(-) dH2O
0.00
0
0.00
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18

For each probe at 25x volume

- probe, 20x conc.
- mastermix, 2x
- dH2O

12.5
125
12.5

final volume total

needed per plate (with
extra volume just in
case)
4.24
5.14
4.00
9.00
20.00
6.00
4.24
2.32
4.24
10.29
18.00
2.67
9.60
10.29
10.29
20.00
20.00
20.00
5.76
0.00

bring to volume with dH20 combined volume

67.76
72.00
66.86
72.00
68.00
72.00
63.00
72.00
52.00
72.00
66.00
72.00
67.76
72.00
69.68
72.00
67.76
72.00
61.71
72.00
54.00
72.00
69.33
72.00
62.40
72.00
61.71
72.00
61.71
72.00
52.00
72.00
52.00
72.00
52.00
72.00
66.24
72.00
72.00
72.00