Pd:2
12/16/16
Ms. Leno.
The result that occurs with enzyme catalase control and experimental trial.
During the lab process our team conduct an enzyme activity experiment. An enzyme
is known as protein that speeds up chemical breakdown of food. There are many
different types of enzymes that help catalyze chemical reaction. In other words, proteins
increase the rate of reaction. The experiment is based on the enzyme catalase to
investigate how pH will affect the reaction causing it to increase as it breaks down
hydrogen peroxide. Then, the H2O2 is decomposed to water and oxygen. In the lab
procedure our team came up with a hypothesis predicting that if the pH buffer of 7 is
added to the H2O2 of 1.5% then, it would increase the rate of reaction. Which typically
meant that our prediction could change as we increase the pH buffer to a larger amount
into the H2O2.
Catalase Lab Procedure:
Computer with Internet access and Vernier LoggerPro software
LabQuest Mini
Vernier Gas Pressure Sensor
29. Click File, Save As, and save with the file name:
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Logger Pro Data
Email the graph to Ms. Lenowitz by following these final steps!
30. Click File and choose Print Graph. In the drop down menu of printers, choose
32. Open your MESA email and Compose a new email to
glenowitz@mesacharter.org
33. Attach the PDF to the email.
34. Press send!
Data Analysis:
The result of the experimental trial supported the original hypothesis which is if the pH
buffer of 7 is added to the H2O2 1.5% then, it would increase the rate of reaction. In the
experimental trial there were a higher slope of ( m=0.01347 kPA/min) due to the amount
of pH that was added to the hydrogen peroxide to increase the reaction. While, the first
control trial wasnt accurate because of the slope of (m= -0.1249 kPa/min) maybe it is
due to the amount of pressure that cause the slope went negative reaction. The result
make sense and was accurate because the hydrogen peroxide and pH buffer with a higher
amount causes the enzyme and H2O2 increase in the gas pressure . In the end, the lab
procedure went smoothly until the group had decided not to support as a team to find the
accurate slope for the trial to see if the reaction would go higher or lower.