KARYA TULIS ILMIAH

UJI ANTIINFLAMASI INFUSA SAMBILOTO HERBA

(Andrographis Paniculata) PADA MENCIT JANTAN SECARA
IN VIVO

Oleh :

ANDRI PRIYOHERIANTO,S.Farm., Apt.

AKADEMI FARMASI MITRA SEHAT MANDIRI

SIDOARJO
2012

UJI ANTIINFLAMASI INFUSA SAMBILOTO HERBA

(ANDROGRAPHIS PANICULATA)PADA MENCIT
JANTAN SECARA IN VIVO

INTISARI

Sambiloto adalah tanaman yang mempunyai rasa sangat pahit dan memiliki banyak
khasiat.Salah satunya di duga sebagai antiinflamasi, pengertian dari inflamasi adalah
suatu respon protektif tubuh terhadap cidera atau jejas. Salah satu tanda inflamasi
adalah edema. Sambiloto herba mengandung flavonoid yang berfungsi untuk
menghambat edema. Penelitian ini bertujuan untuk mengetahui adanya aktifitas
antiinflamasi sambiloto herba (andrographis paniculata ) terhadap edema pada paha
kaki mencit. Dalam penelitian ini menggunakan 15 ekor mencit yang dibagi menjadi 3
kelompok, yaitu kelompok kontrol yang diberi aquades, kelompok infusa dosis 30
mg/kgBB, dan kelompok infusa dosis 50 mg/kgBB. Pemberian aquades dan infusa
sambiloto dilakukan secara per oral lalu di ikuti dengan penginjeksian asam asetat 1%
untuk membuat terjadinya edema. Pengukuran besar edema dilakukan dengan
menggunakan tali rafia lalu diukur beswar edema yang terjadi dengan penggaris,
pengamatan dilakukan selama tiga jam. Data yang diperoleh dianalisis secara statistik
dengan anava one way, jika terdapat perbedaan antara perlakuan dilanjutkan dengan uji
LSD. Hasil penelitian menunjukkan pemberian infusa sambiloto herba dosis 30 mg/kg
BB dan dosis 50mg/kg BB dapat menyebabkan penurunan edema .
Kata kunci : infusa sambiloto, in vivo, edema, inflamasi

ABSTRACT
Sambiloto is a plantthat has avery bitter taste and has many benefits. One of the min
thought as anti-inflammatory, understanding of inflammation is a protective response of
the body again stinjuryor lesion. One sign of inflammation was edema. Bitter herb
contains flavonoids that function to inhibit edema. This study aimed to investigate the

anti-inflammatory activity of bitter herbs (andrographis paniculata) against edema in

mice thigh. In this study using 15 mice were divided into 3 groups, the control group
was given distilled water, infusion dose group 30 mg / kg, and the infusion dose groups
of 50 mg / kg. Giving distilled water and bitter infusion is done orally and reinjection
followed by 1% acetic acid to make the occurrence of edema. Edema of the
measurement is done using a rope and then measured beswar edema that occurs with a
ruler, observations were made during three hours. The data obtained were statistically
analyzed by one-way ANOVA, if there is a difference between treatments followed by
LSD test. The results showed granting bitter herb infusion dose of 30 mg / kg and
50mg/kg doses of BB can cause a decrease in edema.
Key word :sambiloto infusion, in vivo, edema, inflamation

DAFTAR ISI
Halaman

HALAMAN JUDUL ...........................................................................................

HALAMAN PENETAPAN PENGUJI .............................................................

ii

HALAMAN PENGESAHAN ............................................................................. iii

KATA PENGANTAR ......................................................................................... iv
INTISARI ........................................................................................................... vi
ABSTRAK ........................................................................................................... vii
DAFTAR ISI .....................................................................................................

viii

DAFTAR TABEL ...............................................................................................

DAFTAR LAMPIRAN ....................................................................................... xi

BAB 1.

BAB 2.

PENDAHULUAN
1.1

Latar Belakang ........................................................................

1.2

Rumusan Masalah ...................................................................

1.3

Tujuan Penelitian ......... .......................................................... 3

1.4

Manfaat Penelitian ..................................................................

TINJAUAN PUSTAKA
2.1

Sambiloto ..................................................................................

2.1.1 Klasifikasi Sambiloto .....................................................

2.1.2 Deskripsi Tanaman sambiloto ........................................

2.1.3 Kandungan kimia sambiloto ...........................................

2.1.4 Khasiat dan manfaat ......................................................

2.2 Inflamasi ......................................................................................

2.2.1 Etiologi ..........................................................................

2.2.2 Patofisiologi.................................................................... 10
2.2.3 Anti inflamasi ................................................................. 11
2.2.4 Penggolongan anti inflanasi ........................................... 12
2.3 Ekstraksi ...................................................................................... 17
2.3.1 Pemilihan penyari ........................................................... 17
2.3.2 Metode ekstraksi............................................................. 18
2.4 Hewan uji ( mencit ) ................................................................... 22
2.5 Uji anova ...................................................................... ........... 22

BAB 3.

METODE PENELITIAN
3.1

Jenis Penelitian ........................................................................ 26

3.2

Rancangan Penelitian.............................................................. 26

3.3

Variabel Penelitian .................................................................. 26

3.3.1 Variabel Bebas ............................................................... 27
3.3.2 Variabel Terikat .............................................................. 27
3.3.3 Variabel Terkendali ........................................................ 27

3.4

Waktu dan Tempat Penelitian. .............................................. 27

3.5

Alat dan Bahan ........................................................................ 27

3.5.1 Alat ................................................................................ 27
3.5.2 Bahan ............................................................................ 27

3.6

Prosedur Penelitian ................................................................ 28

3.6.1 Penyiapan hewan uji ....................................................... 28
3.6.2 Pembuatan simplisia sambiloto...................... ................ 28
3.6.3 Pembuatan edema .......................................................... 30
3.6.4 Pengujian efek anti inflamasi....................................... .. 30

3.7
BAB 4.

Analisa data .......................................................... .................. 31

HASIL DAN PEMBAHASAN

4.1

Infusa sambiloto
4.1.1 uji anti inflamasi sambiloto herba ................................... 32

BAB 5.

KESIMPULAN DAN SARAN

5.1 Kesimpulan .................................................................................. 38
5.2 Saran ........................................................................................... 38

Daftar Pustaka ................................................................................................... 39

LAMPIRAN