Varian
Cary Eclipse
Fluorescence
Spectrophotometer
Who is Varian?
Varian, Inc. has been supplying essential
tools for scientists engaged in drug
discovery, life science, genetic engineering,
neuroscience studies, health care,
environmental analysis, industrial R&D and
quality control, and academic applications
for over 50 years. Our range of UV-VisibleNIR spectrophotometers, fluorescence
spectrophotometers, NMR spectrometers,
NMR imaging spectrometers, gas
chromatographs, gas chromatograph/mass
spectrometers, high-performance liquid
chromatographs, atomic absorption
spectrometers and inductively coupled
plasma spectrometers are supported by
extensive consumable supplies and services
that will keep your laboratory running
efficiently. We are a global organization
with offices or dealers in over 70 countries.
How does Cary fit in?
The Cary name has been associated with
high performance spectrophotometers for
over 50 years. From the Cary 11 introduced
in 1947 to the Cary 50, 100, 300, 400,
500 families introduced in 1997, the name
Cary has been synonymous with excellence
and performance. Cary instruments have
become the standard for researchers
wanting to push the limits of their
measurement techniques. Cary instruments
have also found a home in many of the
routine laboratories around the world
where their reliability and ease-of-use are
vital. The Cary Eclipse fluorescence
spectrophotometer fits well within the Cary
range, offering the excellent specifications
and modern software that has become the
Cary standard.
Whats inside?
Excellent sensitivity is
a result of using an
intense Xenon flash
lamp, coupled with
optimized grating blaze
angles and coatings
which ensure sensitivity
across the whole
wavelength range.
Photosensitive samples
are not exposed to
continuous light as the
Xenon flash lamp
flashes only to acquire
a data point.
Reduced volume
cuvettes (40 L and
400 L) are available.
The horizontal beam
profile ensures
excellent sensitivity
even with such
low volumes.
The Schwarzchild
collection optics
capture a large portion
of the light from the
powerful Xenon flash
lamp and direct it
through the sample.
This results in excellent
sensitivity and low
signal noise.
Red-sensitive
Photomultipler tube
detectors extend
sensitivity up to 900 nm
without sacrificing
UV performance.
Various built-in
filters on both
monochromators means
that you dont have
to buy your own to
minimize scattering or
exclude second-order
stray light.
Single window
operation: All controls
can be accessed from
this window, making
operation quick
and easy.
Data can be
automatically exported
to a spread sheet at the
end of the measurement.
Prepare to publish!
You can cut and paste
graphs from the Cary
Eclipse software into
any word processing
package, including any
bitmaps or text you
may have added to
the graph.
Three modes of
operation are offered:
fluorescence,
phosphorescence and
bio-/chemi-luminescence.
Create an excitation/
emission matrix (EEM)
or 3-D plot to see
exactly what is
happening in your
sample, e.g., what
happens to the emission
intensity as the
excitation wavelength
is changed? A 3-D plot
shows you the
interaction between
emission wavelength,
excitation wavelength
and fluorescence
intensity. The 3-D data
can be sliced to
produce single
excitation or emission
scans, or contour plots
can be created to
show the number of
emitting species.
Perform synchronous
scans with a constant
wavelength or
wavenumber difference
between monochromators
to resolve multicomponent mixtures
Cary Eclipse
software-Bio
The Bio package
contains the following
modules: Kinetics, Lifetimes,
Scan, Concentration, Simple
Reads, Advanced Reads, Align, GLP
Admin, Validate, System information
and ADL Shell (for more information
about each of these modules, refer to the
Cary Eclipse specifications brochure).
The Cary Eclipse provides some unique
capabilities, including:
Fast Kinetics
The Cary Eclipses electronic design,
coupled with the use of the Xenon flash
lamp, allows steady-state fluorescence
data to be collected at up to 80 points
a second. Use the rapid mix accessory to
analyze reactions that are over in less
than 1-2 seconds.
Data security guaranteed
Some spectrophotometers only store data
at the end of a run, so if the power fails
during the analysis you have to start again.
The Cary Eclipse software stores data as it
is acquired, so even if someone accidentally
pulls the power plug, youll still have the
data up to that point.
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Need an extension?
If you decide during an assay that you set
an incorrect end time, you can extend the
length of the assay without stopping the
measurement. You can also use the pause/
continue function to add reagents without
introducing spikes in your data by opening
the sample compartment lid.
Why do 4 experiments when you
can do 1?
The Dwell time option in the Kinetics
software allows you to measure multiple
data points per cell before moving to the
next cell. Use the multicell holder to
monitor four very fast reactions in the
one experiment.
Intracellular ion concentrations
Intracellular ion concentration analysis or
pH measurements are also possible with the
Cary Eclipse. Using the fast slew rate of the
monochromators, data can be acquired and
displayed in real time within a second for
ratiometric measurements or every 12.5 ms
for single wavelength dyes.
Perform time-resolved
measurements with
a 2 s gate time!
This example shows
the time-resolved
fluorescence decay
of Europium (III) over
a 2 ms time period.
In being able to
collect 1000 data
points, rate calculations
can be performed
with a high precision
and accuracy.
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Safety
It is Varians policy to manufacture safe
products and to meet all legal
requirements governing the design,
manufacture and sale of safe products.
As with all similar products, some or all
of the following hazards may be present:
high temperatures, high pressure gases,
explosive gases, magnetic and radio
frequency radiation, UV light and
electricity. Each product is designed to
protect operators from potential hazards.
Varian supplies instructions which
describe the correct procedures for the
operation and maintenance of each
product. The Cary Eclipse fluorescence
spectrophotometer is designed to measure
the emission of light from materials.
Automated polarizer
Manual polarizer
Microplate reader
PCB-150 peltier
water bath
Temperature controller
Temperature probe
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Sales offices
Australia
Varian Australia Pty Ltd
Mulgrave, Victoria
Tel 1 300 368 274
Austria
Varian Ges.m.b.H.
Vsendorf bei Wien
Tel (1) 69 99 669
Belgium
Varian Belgium NV/SA
Zaventem
Tel (0) 15 55 6460
Brazil
Varian
Indstria e Comrcio
Sao Paulo
Tel (11) 820 0444
Canada
Varian Canada Inc.
Mississauga, Ontario
Tel 1 800 387 2216
or (905) 819 8181
Europe (other)
Varian International AG
Bergen op Zoom
Tel (0164) 282800
France
Varian S.A.
Les Ulis Cdex
Tel (1) 69 86 3838
Germany
Varian GmbH
Darmstadt
Tel (6151) 703 362
Italy
Varian SpA
Torino
Tel (11) 997 9111
Japan
Varian Japan Ltd
Tokyo
Tel (3) 52321239
Korea
Varian Technologies
Korea Ltd
Kangnam Gu,
Tel (2) 345 22452
South America
Varian
Indstria e Comrcio
Sao Paulo
Tel (11) 820 0444
Spain
Varian Ibrica S.L.
Madrid
Tel (91) 472 7612
Sweden
Varian AB
Solna
Tel (8) 445 1620
Switzerland
Varian International AG
Zug
Tel (41) 749 88 44
Mexico
Varian S.A.
Mexico City,
Tel (5) 533 5985
Netherlands
Varian Nederland B.V.
Bergen op Zoom
Tel (0164) 282800
United States
Varian Associates
Walnut Creek
Tel 1 800 926 30 00 or
(925) 939 2400
Russian Federation
Varian Associates Inc.
Moscow
Tel (095) 9374280
Other countries
Varian Australia Pty Ltd,
Australia
Tel (3) 9560 71 33
Fax (3) 9560 79 50