Oleh:
dr. Putu Indah Andriani, S.Ked
KATA PENGANTAR
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Puji syukur penulis panjatkan kepada Tuhan Yang Maha Esa atas berkat,
kasih dan rahmat-Nya penulis dapat menyelesaikan proposal penelitian yang
berjudul Efek Formula Poliherbal Ekstrak Hibiscus Rosa Sinensis dan Vitis
Vinifera Terhadap Pertumbuhan Folikel Rambut. Proposal penelitian ini dibuat
dalam rangka mengikuti ujian seleksi program pendidikan dokter spesialis
combine degree di program pasca sarjana Universitas Udayana tahun 2013.
Pada kesempatan ini penulis mengucapkan terima kasih yang sebesar-
besarnya kepada :
1. Seluruh dosen di Universitas Udaya selama penulis menempuh pendidikan
semua pihak yang telah membantu dalam pelaksanaan dan penyelasaian proposal
penelitian ini.
Penulis
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ABSTRAK
Rambut adalah salah satu bagian penting bagi tubuh manusia. Alopecia merupakan
suatu kondisi dimana rambut mulai menipis kemudian rontok dan akhirnya
menyebabkan kebotakan. Terapi untuk perawatan alopecia sudah banyak
dikembangkan, antara lain dengan menggunakan metode laser, operasi, maupun
obat-obatan sintetis seperti minoxidil dan finasteride yang berfungsi untuk
merangsang pertumbuhan rambut. Tapi sayangnya pilihan metode terapi yang
digunakan ini memerlukan biaya yang cukup besar dan belum menjangkau bagi
sebagian besar masyarakat. Sehingga perlu dikembangkan suatu ekstrak alami dari
tumbuhan yang mudah diperoleh dan bisa berfungsi untuk merangsang
pertumbuhan rambut. Ekstrak tumbuhan yang bisa digunakan adalah daun Hibiscus
rosa sinensis dan biji Vitis vinifera. Penelitian ini bertujuan untuk mengetahui
pengaruh pemberian ekstrak daun Hibiscus rosa sinensis, biji Vitis vinifera, ataupun
kombinasi keduanya terhadap pertumbuhan rambut.
Kata Kunci: Hibiscus Rosa Sinensis, Vitis Vinifera, Folikel Rambut, Alopecia
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ABSTRACT
Effect Polyherbal Formulation Hibiscus rosa sinensis and
Vitis vinifera Extract on Hair Growth
Hair is the one of the impotents parts of human body. Alopecia is a condition when
the hair become thinner, fall off, and bald. Now days, there are many treatment for
alopecia, such as laser, operation, and used synthetic agent like minoxidil and
finasterine for stimulating hair growth. But unfortunately, this kinds of treatment
modalities are too expensive for most of the society in Indonesia. For these reason,
this research try to develop some alternative herbal formulation for hair growth.
Herbal formulation will use in this research are extract from the Hibiscus rosa
sinensis leaf and from Vitis vinifera seed . The purpose of these research are to
know the effect from the extract Hibiscus rosa sinensis leaf, Vitis vinifera seed, and
combination both of them to stimulate hair growth.
There are many kinds factors involved in hair growth. These factor can be divine by
intrinsic factor and extrinsic factor. The intrinsic factors consist of genetic, androgen
hormone, and autoimmune. The extrinsic factors consist of UV radiation, chemical
radiation, chemmotherapy effect, bacterial or fungal infection in the scalp. All of
these factors finally will be increase Reactive Oxygen Species, Nitric Oxide, and
many kinds of cytokine pro-inflammatory. Extract from the Hibiscus rosa sinensis
leaf and Vitis vinifera seed, contain antioxidant and anti-inflammatory that can be
used to inhibit this inflammation process. Extract from Hibiscus rosa sinensis also
contain anti-androgen compound that can inhibit androgen hormone bond with
androgen receptor in hair follicle. Extract from the Vitis vinifera seed also contain
high concentration oligomer prothocyanidins, which is important for hair growth.
Combination of these kind polyherbal formulation will be give synergy effect for
hair growth.
This research will use Randomised Pre-Post Test Control Group Design. Samples
for this research are 32 albino mice. The samples will be divine into four groups,
each group consist of 8 albino mice. Group 1 (Use Vaseline cream), group 2 (use
cream which is contain 5% extract Hibiscus rosa sinensis leaf), group 3 (use cream
which is contain 5% extract Vitis vinifera seed, and group 4 (use cream which is
contain 5% combination extract Hibiscus rosa sinensis leaf and Vitis vinifera seed).
Measurement of parameters hair follicle growth like length and density of the hair
in 1 cm2 area will be doing in 15th, 20th , 30th day after apply these cream. Histology
examination will be doing in 30th day after apply these cream.
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