RINGKASAN

EFEK PEMBERIAN SUPLEMEN WHEY PROTEIN TERHADAP KADAR

PROTEIN KARBONIL PLASMA MENCIT (MUS MUSCULUS) YANG
DIBERI PERLAKUAN AKTIVITAS EKSENTRIK

Protein karbonil merupakan marker oksidasi protein oleh radikal bebas di dalam tubuh.
Radikal bebas dalam tubuh dapat terbentuk oleh karena aktivitas fisik salah satunya aktivitas
eksentrik. Radikal bebas yang meningkat melebihi kadar antioksidan dalam tubuh akan
mengoksidasi berbagai macam protein di dalam tubuh yang kemudian akan membentuk
gugusan karbonil yang disebut dengan protein karbonil. Kadar protein karbonil dalam tubuh
diketahui meningkat setelah melakukan aktivitas eksentrik (Lee et al, 2002; Powers &
Jackson, 2008).
Whey protein merupakan protein yang berasal dari susu sapi, yang diketahui dapat
menghambat stress oksidatif akibat aktivitas fisik dan juga mampu mengimbangi kehilangan
protein otot dengan meningkatkan stimulasi protein otot. Asam amino sistein yang terdapat
dalam whey protein diketahui mampu meningkatkan sintesis glutation endogen. Glutation
merupakan antioksidan endogen yang poten untuk menangkal radikal bebas (Counous, 2000;
Wang et al, 2014).
Penelitian ini merupakan penelitian eksperimental dengan randomized post-test only
control group design. Penelitian ini menggunakan 30 ekor mencit jantan (Mus musculus) galur
Balb/c, dibagi menjadi lima kelompok, yaitu K1 (placebo, langsung diambil darah), K2
(placebo selama 3 hari + lari downhill, darah diambil 4 jam setelah lari), K3 (whey protein
selama 3 hari + lari downhill, darah diambil 4 jam setelah lari), K4 (placebo selama 3 hari +
lari downhill, darah diambil 72 jam setelah lari), K5 (whey protein selama 3 hari + lari
downhill, darah diambil 72 jam setelah lari). Pengambilan darah pada mencit dilakukan
melalui jantung, sehingga dilakukan prosedur anastesi dan pembedahan terlebih dahulu.
Setelah darah terambil, mencit di korbankan. Pengambilan darah tersebut digunakan untuk
pemeriksaan kadar protein karbonil plasma dengan teknik proteomik menggunakan
microplate reader (ELISA).
Data rerata SD protein karbonil plasma pada K1 adala 0,59 0,65; pada K2 adalah 1,24
0,11; pada K3 adalah 1,22 0,17; pada K4 adalah 1,24 0,33 dan pada K5 adalah 0,19 0,13. Uji
normalitas kadar protein karbonil plasma menunjukkan data berdistribusi normal tetapi uji
homogenitas menunjukkan data tidak homogen. Uji beda kadar protein karbonil plasma
menggunakan uji Brown-Forysthe dan dilanjutkan dengan uji Games-Howell. Hasil penelitian
ini menunjukkan bahwa pemberian whey protein 3 mg/g BB mencit selama 3 hari menurunkan
kadar protein karbonil plasma setelah aktivitas eksentrik.

Protein carbonyls are markers of protein oxidation by free radicals in the body. Free
radicals in the body can be formed by physical activity, one eccentric activity. Increased levels
of free radicals exceeds the antioxidant levels in the body will oxidize a wide range of proteins
in the body, will then form a carbonyl group of the so-called protein carbonyl. Protein
carbonyl levels in the body proved to be increased after the eccentric exercise

Whey protein is a protein derived from cow's milk, which is known to inhibit oxidative
stress due to physical activity and also able to offset the loss of muscle proteins by increasing
the stimulation of muscle protein. Cysteine amino acids contained in whey protein known to
increase endogenous glutathione synthesis. Glutathione is a potent endogenous antioxidant
for free radical protection

This study is a randomized experimental post-test only control group design. This
study used 30 male mice (Mus musculus) strain Balb / c, are divided into five groups, namely
K1 (placebo, directly drawn blood), K2 (placebo for 3 days + run downhill, blood is taken 4
hours after a run), K3 (whey protein for 3 days + run downhill, blood is taken 4 hours after a
run), K4 (placebo for 3 days + run downhill, blood was taken 72 hours after the run), K5
(whey protein for 3 days + run downhill, blood is taken 72 hours after the run). Blood
sampling in mice conducted through the heart, so do anesthesia and surgical procedures
beforehand. Blood sample used for examination of plasma levels of protein carbonyls with
proteomics techniques using microplate reader (ELISA)

Mean SD plasma protein carbonyls in K1 was 0.59 0.65; on K2 was 1.24 0.11;
the K3 is 1.22 0.17; Q4 was 1.24 0.33 and the K5 was 0.19 0.13. Normality test plasma
levels of protein carbonyls showed normal distribution but homogeneity tests showed the data
are not homogeneous. Different test plasma levels of protein carbonyls using test-Forysthe
Brown and continued by Games-Howell. The results of this study showed that administration
of whey protein 3 mg / g for 3 days decreased plasma levels of protein carbonyls after
eccentric exercise.
 ABSTRAK
EFEK PEMBERIAN SUPLEMEN WHEY PROTEIN TERHADAP KADAR
PROTEIN KARBONIL PLASMA MENCIT (MUS MUSCULUS) YANG
DIBERI PERLAKUAN AKTIVITAS EKSENTRIK

Latar belakang: Protein karbonil merupakan marker terjadinya okidasi protein di dalam
tubuh. Oksidasi protein terjadi oleh karena kadar ROS yang meningkat pada kondisi stress
oksidatif. Whey protein mengandung asam amino sistein, yang dapat mensintesis glutation
endogen, sehingga mampu menghabat terjadinya stress oksidatif.
Tujuan: Mengetahui efek suplemen whey protein terhadap penurunan kadar protein karbonil
plasma pada aktivitas fisik eksentrik
Metode: Penelitian ini merupakan penelitian eksperimental dengan randomized post-test only
control group design dan menggunakan 30 ekor mencit jantan, dibagi menjadi lima kelompok,
yaitu K1 (placebo, langsung diambil darah), K2 (placebo selama 3 hari + lari downhill, darah
diambil 4 jam setelah lari), K3 (whey protein selama 3 hari + lari downhill, darah diambil 4
jam setelah lari), K4 (placebo selama 3 hari + lari downhill, darah diambil 72 jam setelah lari),
K5 (whey protein selama 3 hari + lari downhill, darah diambil 72 jam setelah lari).
Pengambilan darah tersebut digunakan untuk pemeriksaan kadar protein karbonil plasma.
Hasil: Analisis statistik uji Post Hoc Games Howell menunjukkan kadar protein karbonil
menurun signifikan pada kelompok yang diberi whey protein, dibandingkan dengan kelompok
yang tidak diberi whey protein terutama pada jam ke 72 setelah lari downhill (p = 0,006)
Kesimpulan: Terdapat efek positif signifikan pemberian whey protein terhadap penurunan
kadar protein karbonil plasma pada aktivitas fisik eksentrik
Kata kunci: whey protein, protein karbonil, aktivitas eksentrik
Background: Protein carbonyls are markers of protein oxidation by free radicals in the body.
Protein oxidation occurs due to elevated levels of ROS in oxidative stress conditions. Whey
protein contains the amino acid cysteine, which can synthesize endogenous glutathione, so as
to inhibit the oxidative stress.

Objective: to determine the effects of whey protein supplementation on plasma protein

carbonyls levels in eccentric exercise

Methods : This study is a randomized experimental post-test only control group design. This
study used 30 male mice (Mus musculus) strain Balb / c, are divided into five groups, namely
K1 (placebo, directly drawn blood), K2 (placebo for 3 days + run downhill, blood is taken 4
hours after a run), K3 (whey protein for 3 days + run downhill, blood is taken 4 hours after a
run), K4 (placebo for 3 days + run downhill, blood was taken 72 hours after the run), K5
(whey protein for 3 days + run downhill, blood is taken 72 hours after the run). Blood sample
used for examination of plasma levels of protein carbonyls

Results: Statistical analysis Games Howell Post Hoc test showed levels of protein carbonyls
decreased significantly in the group given whey protein, compared with the group not given
whey protein, especially on the hour to 72 after the downhill run (p = 0.006)

Conclusion: There is a significant positive effect on the administration of whey protein

decreased levels of plasma protein carbonyls in eccentric exercise

Key words: whey protein, protein carbonyls, eccentric exercise