4 MAY-2016
ABSTRACT- The present investigation was carried out to the effect of Fenvalerate on haematological parameters in
freshwater fish Channa marulius using standard methods. Fish was exposed to (1/4th LC50) sub lethal concentration of
Fenvalerate (0.086ppm) for 96h.The blood sample were obtained from both, control and experimental fish and assayed
haematological parameters (Total Erythrocytes count, TEC; Total Leucocytes count, TLC; Haemoglobin, Hb; Packed cell
volume, PCV; Mean corpuscular volume, MCV; Mean Corpuscular Haemoglobin and Mean Corpuscular Haemoglobin
Concentration; MCHC). The result revealed that TEC, Hb percentage, PCV and MCHC counts were significantly
decreased, whereas TLC, MCV and MCH increased slightly in experimental fish. The study has thus indicated marked
changes in blood of Channa marulius after exposure to Fenvalerate.
Key-words- Fenvalerate, Hematology, Channa marulius, Haemoglobin, Sub lethal
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INTRODUCTION
The blood parameters in fishes are influenced by many Due to injudicious and indiscriminate use of agrochemicals,
factors viz. quality of water, temperature, the sex, size, fertilizers, pesticides, and insecticides to boost crop
season and age of fishes are directly reflected production with the sole aim of getting more yields. Water
haematological parameters of the fishes (Bhagat et al., bodies like ponds, lakes and rivers are continuously getting
1986). The study of fish blood parameters are important for polluted normally these pesticides reaches an
determining factors related to its physiological capacity aquatic environment through surface runoff and sediment
(Affonso, 2001; Wells et al., 2005). transport from treated effluents (Bauman, 1981).
Blood parameters are considered path physiological Direct application of pesticides to water bodies to control
indicators of the whole body and therefore are important in the inhabiting pests. Spray drift from normal agricultural
diagnosis the structural and functional status of fish operation and through heavy rainfall. In all cases these
exposed to toxicants (Adhikari et al., 2004). A number of chemicals may directly toxic as well as deteriorate the
hematological parameters such as RBC, WBC, and water quality by changing its physicochemical nature and
haemoglobin content and so on, are used to assess the cause ecological imbalance leading to health hazards to
functional status of the oxygen carrying capacity of the different types of aquatic organisms in general and fishes in
blood. Pollution of aquatic ecosystem by chemical used in particular (Tripathi, 1992). The herbicide can cause death
industry and agricultural is increasing day by day. of fishes either directly or due to saturation by destruction
of food chain many herbicides have shown to effect the
Access this article online growth and reproduction of fishes with evidence of tissue
Website: DOI: damage (Dhasarathan et al., 2000). The present study was
www.ijlssr.com 10.21276/ijlssr.2016.2.3.13 under taken to analyze the impact of 1/4th sub lethal
Quick Response Code: concentration of Fenvalarate on haematological parameters
of fresh water fish Channa marulius (Ham Buch).
(Mustak and Natrajan,1992),Cyprinus carpio exposed to and Subhisha, 2010). The causes of depletion in the Hb
Dimethoate (Chauhan, et al.,1994). Ramesh and Manvalara content in Puntius conchonius are supposed to
Manujan, (1992) reported decrease in Hb, TEC in degeneration, depression and destruction of the blood
Malathion exposed fresh water carp cyprinus carpio and forming material by the piscicidal compound (Patole and
sumithion exposed to Clarias batrachus respectively. Bhoi, 2013).
Anaemia induced stress may also be due to blood cell
injury and disrupted hemoglobin synthesis (Binna Kumari
Table-1: Haematological parameters of fresh water fish Channa marulius exposed to 1/4th sub lethal concentration
of Fenvalerate (0.086ppm) insecticide
WBC 4.25 0.65 3.80 0.18 4.69 0.3 5.11 0.09 6.38 0.52
(103/mm 3) (-11.84) * (9.38) * (16.15) * (33.38) **
PCV (%) 30.9 4.1 33.3 2.7 28.3 1.2 26.250.75 23.93 2.57
(7.20) * (-9.18)NS (-17.71) * (-29.12) **
MCH(Pg) 42.1 3.5 45.2 3.3 41.3 21 50.2 2.6 43.36 6.64
(6.85) NS (-1.93)NS (16.13) * (2.90) NS
MCHC(g /dl) 34.1 2.3 28.4 1.6 29.6 1.0 27.040.66 23.53 1.77
(-20.07) * (-15.20) * (-26.10) * (-44.92) **
Effect on PCV 42.1 pg. The values were decreased in 48 h. While the
The amount of PCV of exposed C. marulius after the 24 h, values are increased in 24 h,72 h and 96 h than over
48 h, 72 h and 96 h exposure were 33.3, 28.3, 26.25, control.
23.93% and mean control was 30.9 %. It was increase in Effect on MCHC
24h.Whereas in 48h, 72 h and 96 h was significantly The amount of MCHC in the blood of the fish recorded as
decreased from exposure period to control over. 28.4, 29.6, 27.04, 23.53g/dl respectively and means control
Effect on MCV value was 34.1 g/dl was to be found. The MCHC was
The amount of MCV of the fish exposed to sub lethal decreased from control to exposure period. Atamanalp et al,
concentration of Fenvalerate after the 24 h, 48 h, 72 h and (2003), Atmanalp and Yanik, (2003) found a significant
96 h exposed period revealed 163, 165, 172,184m3 increase in the level of RBC and significant decrease in
respectively and mean control was 122 3 respectively. MCH, MCHC, thrombocytes count and erythrocyte
The MCV contain in exposure period was significantly sedimentation rate in rainbow trout (Oncorhynchus mykess)
increased over control. following Cypermethrin and Mancozeb acute exposure. A
Effect on MCH decrease in important blood parameters has been reported
The amount of MCH in the blood of the fishes exposed during exposure to various pesticides in fish (Saxena and
to1/4th sub lethal concentration (0.086ppm) of Fenvalerate Seth, 2002).
for 24 h, 48 h, 72 h and 96 h was found to contain 45.2, The decrease in the above parameters indicates stage of fish
41.3, 50.2 and 43.36 pg respectively and mean control was caused due to decreased erythropoetic activity or increased
http://ijlssr.com IJLSSR 2015 All rights are reserved Page 3
Int. J. Life. Sci. Scienti. Res., VOL 2, ISSUE 3
destruction of red blood cells further support in favors of proximate composition of the fish Cyprinus carpio. Indian J.
this comes from the decrease noted PCV. Decrease in Hb Environ and Ecoplan 3(e): 339-446.
indicates poor O2 transport by blood caused by damage or [10] Dutta, H.M., Dogra, J.V., Sing, N.K., Roy, P.K., Nasar, S. T.,
due to increased accumulation of CO2 in blood. In the Adhakari, S. Munshi, J.S. and Richmonds, C.K. (1992). Ma-
lathion induced changes in serum protein and haematological
present investigation significant change was recorded in the
parameters of Indian catfish Heteropneustes fosslis
RBC, WBC, Hb, MCV, PCV and MCHC. The reduction in (Bloch);Bull.Environ.Contam.Toxicol. 49:91-97.
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an erythropoetic organ would have lowered those values. pathology.Bailliere Tindall, London, P.23-54.
[12] Gill, T.S., Pande, J. and Tiwari, H. (1992). Effect of
CONCLUSION Endosulfan on the blood and organ chemistry of freshwater
The present investigation show that Fenvalerate caused fish, Barbus conchonitus (Ham). Ecotoxicol.
Environ.Saf.21:80-89.
decrease in the haematological parameter in Channa
[13] Kalair, J.S., Mishra, V.S. and Sing, R. K. (1993). Pesticide
marulius which suggest that the Fenvalerate (synthetic sumithion induced haematological changes in mud eel
pyrethroids) may weak the immune system and result in Amphipnous cuchia; Bio.Mem.19:41-48.
severe physiological problem ultimately to the death of [14] Kumar, S., Lata, S. and Gopal, K. (1999). Deltamethrin
fish. Fenvalerate is a synthetic pyrethroid used to protect induced physiological changes in freshwater catfish Hete-
many fruits, vegetables and field crops against disease, ropneustes fossils; Bull.Envioron.Contam.Toxicol.62:
hence farmer come direct contact it and may impair their 254-258.
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ACKNOWLEDGMENT [16] Patole, S. S. and Bhoi, S.S. (2013). Comparative studies on
The authors are grateful to the principal, S.G. Patil Arts, haematological responses in the fresh water fish, Punctius
Science and Commerce College, Sakri Dist. Dhule (M.S.) conchonius exposed to two piscicidal plants extracts.
for providing necessary laboratory facilities. Also thankful [17] Ramesh, M., Manaralara Manujan, R. and Sivakumari, K.
to Zoological Survey of India, Western Regional Station, (1992). Effect of water hardness and toxicity of Malathion on
Akurdi, Pune (M.S.) for the identification of fish. haematological parameters of the fresh water fish, Cyprinus
carpio, J. Ecotoxicol. Environ. Monit. 2(1): 31-34.
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