Int. J. Life. Sci. Scienti. Res., VOL 2, ISSUE 2, pp: 93-96 (ISSN: 2455-1716) Impact Factor 2.

4 MARCH-2016

Research Article (Open access)

Morphological Study and Role of Gonad Cells in Freshwater Fish- Reproduction

M.V. Gaikwad1*, P.K. Banmeru2 and Y.K.Khillare3
1
Depertment of Zoology, Late Ku. Durga K. Banmeru Science College Lonar
2
Department of Electronics, Late Ku. Durga K. Banmeru Science College Lonar
3
Depertment of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad (MS)

ABSTRACT- Reproduction is a vital process provided to all living individuals by the nature itself to reproduce their
similar one after death. Channa gachua is used due to easily throughout year availability. Male gonad was removed during
different reproductive phases. The nourishing protein contain was recorded by Lowrys method 660nm during matured
male gonad as 0.555 mg/ml. Sertoli cells were observed on their development and parameters were recorded with the help
of phase contrast microscope (Phase contrast-Olympus) as total number of Sertoli cells 150199 in number, diameter of
the cell 0.10.2 perimeter of the Sertoli Cell 0.1 to 0.01 in matured testis on the colour intensity. Same conditions
were used to abnormal male brooder in hatchery the nourishing protein contain was comparatively low about infertile
male brooder in matured gonad as 0.467mg/ml. The development of Sertoli cell and its parameter were comparatively low
as number of Sertoli cells 99120, diameter of the cell 0.0010.005 perimeter of the Sertoli Cell in male brooder. The
lesser development of sertoli cell leads to improper development of Sertoli gives insufficient sperms cell count for
abnormal fertilization.
Key words- Testis, Ovary, Reproduction, Reproductive phases, Supportive cells, Channa gachua
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INTRODUCTION
Reproduction is a vital process provided to all living Fishes has got a complicated reproductive behavior very
individuals by the nature itself to reproduce their similar typical embryonic development. Morphologically, healthy
one after death. Channa gachua is used due to easily testis consists of different organells with the cells lie Sertoli
throughout year availability. Channa gachua is having and Leydigs cells which are really a supportive and
important as much as Indian major carps. It is a good functional unit of gonads.
brooder used in induced breeding process in Chinese The somatic Sertoli cell in fishes plays an essential role in
hatchery and to bring perceptual breeding in it. Reproduc- embryonic determination of a male somatic sex and
tions in any animals are one of the basic functions to spermatogenesis during adult life. One individual Sertoli
propagate the respective population in cyclic manner. cell sullies a clone of developing germ cells with nutrient
*
Address for Correspondence: and growth factors and it is well established that the
Dr. Milind V. Gaikwad number of Sertoli cell. The testes of teleost fishes show
H.O.D.
greater morphological variation than in other vertebrates
Depertment of Zoology
Late Ku. Durga K. Banmeru Science College Lonar, [1-3]. In most cases, testes are a pair of elongated structures
Dist. Buldana, India composed of branching seminiferous tubules embedded in
Received: 27 Jan 2016/Revised: 13 Feb 2016/Accepted: 26 Feb 2016 the stroma. The testis consists of thin-walled tubules or

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Int. J. Life. Sci. Scienti. Res., VOL 2, ISSUE 2

lobules that contain germ cells - the spermatogonia- which of phase contrast microscope (Phase contrast-Olympus) as
are endodermal in origin. Germ cells divide in clusters total number of Sertoli cells 150199 in number, diameter
enclosed by a cyst. Primary spermatogonia - the stem cells of the cell 0.10.2 perimeter of the Sertoli Cell 0.1 to
- which are present throughout the year, divide mitotically 0.01 in matured testis on the colour intensity.
to give rise to secondary spermatogonia which get Same conditions were used to abnormal male brooder in
transformed into primary spermatocytes. They divide by hatchery the nourishing protein contain was comparatively
meiosis and give rise to spermatids from which low about infertile male brooder in matured gonad as
spermatozoa are formed. The seminiferous tubules are 0.467mg/ml.The development of Sertoli cell and its
packed with spermatozoa in the pro-spawning and parameter were comparatively low as number of Sertoli
spawning periods. Vertebrate testis shows a germinal cells 99120, diameter of the cell 0.0010.005
compartment represented by seminiferous tubules perimeter of the Sertoli Cell in male brooder. The lesser
(seminiferous lobules), in which two cell types are found: development of sertoli cell leads to improper development
somatic and germinal. Compared to mammals, Sertoli cell of Sertoli gives insufficient spem cell count for abnormal
supporting capacity in the teleost Nile tilapia is remarkably fertilization.
high [4]. The number of mitotic divisions during Photo 1. Testis in growing phase
spermatogenesis to form primary spermatocytes is also
species-specific Schulz et.al. (2002). In mammals, the ratio
germinal cells: A Sertoli cell (which expresses the Sertoli
cell supporting capacity) is relatively defined for each
species [5-7].
MATERIAL AND METHOD
Channa gachua was used for male gonadal study and
collected from Godavary River near Aurangabad. Fish was
dissected and gonad removed, fixed with help of formal
solution as fixative.
The nourishing protein contain was measured by Lowrys
method 660nm during matured male gonad. Sertoli cells
were observed on their development and parameters were
recorded with the help of phase contrast microscope (Phase
contrast-Olympus). Staining of the section made with
reagents Haematoxyline and Eosin.
Photo 3 - Matured testis phase position
RESULT AND DISCUSSION in Channa gachua (Ham-1822)
Male gonad was removed during different reproductive Fig. 1 Shows Sertoli cells, Ledigs cell and Semniferus
phases. The nourishing protein contain was recorded by tibule
Lowrys method 660nm during matured male gonad as
0.555 mg/ml. Sertoli cells were observed on their
development and parameters were recorded with the help

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Int. J. Life. Sci. Scienti. Res., VOL 2, ISSUE 2

Fig. 2 Shows developing follicles in mature stage of

Female Channa gachua

Table 1. Shows cell parameter

CONCLUSION
Like all mammals teleosts have Sertoli cell and it performs
nursing function for developing and developed sperms. It
also shows phagocytic function for all debris in the testes
due to apoptosis. Improper development of supporting cell
of gonads fails to bring proper fertilization. Sometime may
100X
tend to infertility also.
ACKNOWLEDGEMENT
This study was carried out in financial support (RGNFS), at
the fishery science laboratory at Department of Zoology Dr.
Babasaheb Ambedkar Marathwada University, Aurangabad
and Department of Zoology Late Ku. Durga K. banmeru
Science College Lonar, Dist. Buldana.
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Photo 1. Maturing eggs of Channa gchua
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Int. J. Life. Sci. Scienti. Res., VOL 2, ISSUE 2

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