OF MICROBIOLOGY
Chapter 2
Introduction
Classification of microorganisms
Bacteria, Fungi
Protozoa , Viruses
Chlamydia, Rickettsia
Mycoplasmas
Ureaplasmas
Viruses
Glycoproteins
Viruses
Intracellular parasites
20-400 nm in size
Single-stranded RNA
Plant pathogens
No nucleic acid
Creutzfeldt-Jacob syndrome
Fungi
Prokar Bacteria Eukar- Protozoa
yotes Archeae yotes Algae
Differences of Prokaryotes and
Eukaryotes
Prokaryotes Eukaryotes
No true nucleus True nucleus
Single stranded Chromosome
chromosome not separated from
separated from cytoplasm
cytoplasm
Haploid Diploid
Archaea
Extreme
thermophiles
Extreme halophiles
Pathogens
Action Saprophytes
Bacillus
megaterium
Micrococcus
luteus
Obligate Intracellular Parasites
Rikettsia
Chlamydia
Bacteria of Pharmaceutical Importance
Possess cell
wall
Facultative Mesophiles
anaerobes
Strict Strict
anaerobes aerobes
Fungi
Non-photosynthesizing
plants
Yeasts
Moulds or molds
Yeasts
5-10 m
Unicellular organisms
Binomial Nomenclature
Genus species
Chemoheterotrophs
Obtain C, N, and energy
by breaking down
organic compounds
Microbial Metabolism
Oxidation Reduction
accept loss of
electrons electron
Microbial Metabolism
Glycolysis Fermentation
Aerobic respiration Sugars
Anaerobic Ethanol, CO2, acid,
respiration alcohol
Microbial Metabolism
Proteins
Action of Antibiotic
Toxicity
Ethanol
Primary Organic acids
metabolites
Antibiotics
Secondary Enzymes
metabolite Toxins
Carbohydrates
Secondary Metabolites
Antibiotics
Amylase
Enzymes Biological washing powders
Botox
Toxins Clostridium botulinum
Carbohy- Dextran
drates Plasma expander
Microbial Cultivation
Culture
Function Form
Consistency Composition
Types of CM Based on Function
Basal Enriched
Differential Selective
Basal/Basic/General/Simple/Ordinary
BAP, CAP
BAP
CAP
Differential Media
Indicator or dye
Differentiates groups of
microorganisms
Inhibitory substances
Butt or deep
Slant
Tubed Butt-slant
Broth
Plated
Butt or Deep
Butt-Slant
Broth
Types of CM Based on Consistency
Synthetic
Non-synthetic
Cultivation Methods
Binary fission
Liquid Turbidity
media
Solid Colony
media
Anaerobic Cultivation
Ascorbic Redox
acid indicator
Resazurin
Anaerobic Cultivation
Planktonic
Freely suspended cells in liquid
Sessile
Biofilm or microcolony mode of
growth
Enumeration of Microorganisms
Using
microorganisms Assessing the
in the nutrient
manufacture capability of a
of therapeutic growth medium
agents
Microbial Counting
Total count
Viable count
Pour plate
Membrane filter
Turbidity method
Time consuming
Epifluorescence
ATP luciferin
Impedance
Manometric gas
consumption or production
Epifluorescence Microscope
Epifluorescence
Manometric Method
Microbial Genetics of Bacteria
Genome
Chromosomes (double-
stranded DNA)
Plasmids
Bacteriophage
Bacterial Chromosome
1 mm or 1000-3000
more genes
Multiple
copies
Plasmids
Multiple Two or
copies more
Plasmids
Replicate
independently Pili
Pass from 1
cell to another Conjugative
Sexual
division -
meiosis
Genetic Variation and Gene
Expression
Genotype
Phenotypic adaptation
Genotypic adaptation
Mutation or conjugation
Pharmaceutical Importance of the
Major Categories of Microorganisms
Fungi Protozoa
Viruses
Pathogens
Antibiotics
Sources (streptomycetes)
of Steroids, enzymes, CHO
Contaminant
Opportunistic
pathogens
Protozoa
Potential pathogens
Veterinary medicine
Tropical areas
Preservation of Microorganisms
Cryopro- Glycerol/dimethylsulphoxide
tectant 10% (v/v) in liquid medium