General Biochemistry Lecture

Proteins: Structure-Function & Separation Techniques

14 Dimayacyac, Catrina
____Name____________________________ Score / 35
2C-PH
Yr., Sec., & Course______________________

I. Modified True or False (11 pts)

Encircle the T if the statement is true. Otherwise, if the statement is false, change the underlined
word(s)/phrase to correct the statement. Write your answer on Column I.

Column I
decrease There is an increase in the solubility of the protein to be isolated when its pI is equal to
T
the pH of the protein mixture.
T Arginine will migrate towards the cathode at pH = 12.0.
Last Protein that has a negative net charge will be eluted first in the cation exchange
T
chromatography.
The pores of the semipermeable membrane in dialysis will allow the large molecules to
T
diffuse across the membrane.
T Urea is a denaturing agent which destroys all H-bonds that stabilize a protein.
Chaperones are highly conserved heat shock proteins responsible for protein folding,
T assembly, translocation, and degradation under stress conditions and in many normal
cellular processes.
Sigmoidal Hemoglobin has a hyperbolic O2 binding curve that exhibits positive cooperativity among
T
subunits.
Vitamin C Hydroxylation of proline residues in collagen is catalyzed by prolyl hydroxylase, an
T
enzyme activated by vitamin B.
Sickle-cell anemia is a genetic disease caused by the change of Val to Glu on the sixth
T
amino acid residue of -subunit in hemoglobin.
Glutamate T Given the amino acid sequence, L-I-F-E, the amino acid residue at C-terminal is leucine.
Glyconolysis T Glucagon activates glycogenesis.

II. Matching Type (25 pts)

Match Column A with Column B. Write your answer on the space provided.

COLUMN A COLUMN B
A. Purification Techniques
N
______ 1. Isoelectric focusing L. Based on solubility
N
______ 2. Anion exchange chromatography M. Based on affinity
N
______ 3. Anion exchange chromatography N. Based on charge
O
______ 4. Gel filtration chromatography O. Based on molecular size
L
______ 5. Salting-out
L
______ 6. Reverse phase chromatography

B. Collagen & Elastin

X
______ 7. Random coil conformation W. Collagen
X
______ 8. Desmosine crosslinks X. Elastin
W
______ 9. Triple helix structure Y. Both
Z
______ 10. Globular protein(s) Z. None of the choices
W
______ 11. Has a repeating aa sequence of X-Y-G
Y
______ 12. Found in connective tissues

C. Hemoglobin & Myoglobin

A
______ 13. Tetrahedral arrangement of subunits A. Hemoglobin
B
______ 14. O2 storage B. Myoglobin
C
______ 15. Contains heme C. Both
C
______ 16. Fibrous protein(s) D. None of the choices
A
______ 17. Tetramer

D. Insulin and Glucagon

F
______ 18. Secreted by cells of pancreas E. Insulin
E
______ 19. It is secreted in response to high blood glucose level F. Glucagon
G
______ 20. Maintains glucose homeostasis G. Both
G
______ 21. Endocrine hormones H. None of the choices
E
______ 22. With intra- and inter-disulfide bridges
F
______ 23. Made up of one polypeptide chain

E. Immunoglobulins
R
______ 24. Contains the antigen-binding site R. Variable region
T
______ 25. Contains carbohydrate moiety S. Constant region
T. Heavy chain
U. Light Chain