A. Heating, Ventilation, and Air-Conditioning Systems for Research Laboratories and Animal Facilities:
Heating, ventilation, and airconditioning (HVAC) systems for Research Laboratories and Animal Facilities shall be
designed to maintain the space temperature and humidity at the required set point. These systems shall
automatically adjust, as necessary, to respond to varying space cooling demands in laboratories and animal
facilities. Air-change rates, temperature and humidity shall be closely monitored and controlled on a continuous
basis. The System shall provide adequate ventilation to remove fumes, odors, airborne contaminants, and to
safely operate fume hoods continuously. They shall be designed to maintain relative pressure differentials
between spaces to prevent of cross contamination. Space background noise, generated by HVAC systems, shall
be maintained within the levels prescribed within this document. HVAC systems shall be reliable, redundant and
operate without interruption while being efficient to operate, both in terms of energy consumption and from a
maintenance perspective.
Federal energy conservation standards shall be achieved. An energy monitoring and control system shall be
provided. This document outlines the basis of design and energy conservation compliance requirements. During
the design phase, studies shall be conducted to determine the feasibility of utilizing heat-recovery systems in
research laboratory and animal facility buildings.
Laboratory spaces shall meet the requirements in the Biosafety in Microbiological and Bio-medical Laboratories
published by Center for Disease Control and Prevention and NIH.
Animal Facilities shall meet the requirements in the Guide for the Care and Use of Laboratory Animals
published by the Institute of Laboratory Animal Resources.
Sizing of evaporative type cooling towers shall be based on 1C (2F) higher than the WB temperature shown in
the 0.4% column (10a) shown in the ASHRAE Handbook of Fundamentals.
All outdoor air-cooled condensing equipment shall be designed and selected on the basis of 35C (95F) ambient
temperature.
All outdoor air-cooled condensing equipment shall be designed and selected on the basis of 35C (95F) ambient
temperature
C. Energy Conservation:
The A/E shall utilize the latest edition of the following energy codes and standards to design the exterior envelope
and select HVAC systems, domestic water heating, electrical distribution and illuminating systems:
ASHRAE Standard 90.1
Energy Policy Act 2005.
International Energy Conservation Code.
The Project Officer shall be notified when requirements of the energy conservation codes and standards cannot
be satisfied due to program requirements. New construction or major renovation shall require complete HVAC
and energy simulation modeling. Life cycle cost shall include capital cost factors for chillers and boilers as
provided by NIH, as well as up to date energy costs.
Summer 23 + 1 (73 + 2) 50 + 5
Winter 21 + 1 (70 + 2) 30 + 5
Supply air distribution system shall be designed to minimize turbulence and to avoid having an impact on the
performance of primary containment equipment such as chemical fume hoods and BSCs.
Air outlets shall not discharge into the face of fume hoods or BSCs.
Exhaust grilles and registers shall be located away from supply air diffusers in a manner that creates uniform,
low velocity airflow across the room.
Plenums and air shafts for distribution of supply or exhaust air is prohibited in NIH laboratories and non-
laboratory buildings. Common outdoor air ductwork may be permitted for outdoor air intakes to multiple air-
handling units due to space constraints and building configuration
Corridors shall be provided with conditioned air to maintain design temperatures and as required to make up air
for negatively pressurized rooms opening directly to the corridor. The quantity of conditioned air to the corridors
shall be sufficient to maintain an overall positive building pressure
D.6 Anterooms:
Anterooms are typically located between the laboratory/isolation/protected room and the corridor. The anteroom
has two sets of doors, one door to the laboratory/isolation/protected room and one door to the corridor. These
two doors are interlocked so that only one door can be opened at a time. Depending on the type of isolation
required, the anteroom may be positive, negative or neutral. The use and type of anterooms needs to be
reviewed with NIH/DTR and NIH/DOHS.
Anterooms shall be provided with both supply and exhaust air grilles. In addition, ante-rooms shall be provided
with dedicated supply and exhaust air terminal units/boxes, which would permit the reconfiguration of the
anteroom to accommodate program changes.
Typically, room differential pressure sensors are provided to monitor the pressure differential between the ante
room, the laboratory/isolation/protected room and the corridor. Room pressure differential is set to maintain a
minimum of 2.5 Pa (0.01 in wg). In some cases, room differential pressures may be as high as 12.5 Pa (0.05 in
wg) or greater. Required room differential pressure needs to be reviewed by NIH/DTR and NIH/DOHS.
Program equipment shall comply with NFPA, OSHA, ANSI, NSF, NIH Fume Hoods Specifications requirements
and other applicable standards. Equipment selected shall not contain asbestos, lead or mercury.
The A/E shall obtain equipment requirements so that heat rejection, electrical usage, and other utility
consumption data are included in the design of the HVAC systems. Equipment space requirements shall be
closely reviewed, and layouts shall allow for access to all piping, wiring, and ductwork connections, easy
cleaning, maintenance and repairs.
Mechanical systems shall be designed and detailed so that they do not induce harm to or impede the operating
efficiency of program equipment. Pressure regulators, safety relief valves, gravity drainage facilities, temperature
controls, and backflow protection devices shall be provided as required to protect equipment.
The building temperature control systems / Building Automation Systems (BAS) shall not be use to
operate/control program equipment. The complete control and operation/maintenance strategy for program
equipment shall be closely reviewed against program requirements and with program users.
BSC Class I, Class II-B1 and Class II-B2 are also used in NIH facilities. These particular types of BSCs shall be
hard ducted to a dedicated building exhaust air system. In addition, BSC Class II-B1 and Class II-B2 shall be
factory provided with means of shutting down the BSCs internal fan, whenever the static pressure, in the building
exhaust air system connected to the BSC, drops below the required set point. This is required to avoid having a
positive BSC and positive exhaust ductwork. This will prevent the release of hazardous products into the
laboratory space. Whenever multiple BSC of this type are connected to the same system, each BSC shall be
provided with a dedicated exhaust type air terminal unit. This will ensure the proper exhaust air amount is
maintained through each BSC. Building exhaust air systems serving these BSCs shall include provisions for
increasing the systems static pressure to compensate for loading of the exhaust HEPA filters within the BSC, i.e.
VFDs.
Rooms with ducted BSCs shall be provided with an additional room exhaust air grille connected to a dedicated
exhaust air terminal unit. Whenever the manual isolation damper associated with the BSC is closed, during the
certification process of the BSC, the room ventilation system shall automatically adjust in order to maintain the
negative pressure in the laboratory.
Regardless of class and type, all BSCs, at NIH, shall be provided with unit mounted HEPA filtration of the exhaust
air prior to its discharge to the room space or to the outdoors. All Class II BSCs shall comply with Standard NSF-
49 developed by the National Sanitation Foundation (NSF).
Projects requiring the use of BSCs, regardless of class or type, shall be reviewed and approved by the
NIH/DOHS and NIH/DTR during the design phase of the project.
Fume Hood Type Nominal Hood Width mm (in.) NIH Specification Sec
Vertical Sash Bench 1200 (48) 1800 (72) 11810 (August 2004
Horizontal Sash Bench 1800 (72) 11820 (August 2004
Combination Sash Bench 1800 (72) 11830 (August 2004
In addition, fume hoods shall comply with the testing requirements in the following NIH documents:
NIH Specification Section 15991 On Site Testing Constant Volume Fume Hoods
NIH Specification Section 15992 On Site Testing VAV Fume Hoods
Appendix E.3 Fume Hood Testing and Alarm System.
Fume hoods shall be evaluated AM (as manufactured) under the ANSI/ASHRAE STD 110 and shall meet the
following minimum performance ratings:
Sash design position or positions: 457 mm (18 in.)
Average face velocity: 0.51 m/s (100 fpm) (plus or minus 10%)
Range of face velocities: No point in grid below 0.41 m/s (80 fpm) or above 0.61 m/s (120 fpm). Actual not as
measured.
Average face velocity for sash at 50%: 0.41 (80) to 0.76 m/s (150 fpm).
Average face velocity for sash at 25%: 0.41 (80) to 1.52 m/s (300 fpm)
Performance rating: 0.05 ppm.
Sash movement performance rating: 0.10 ppm.
Response time for VAV hoods: Less than 3 seconds.
Percentage of auxiliary air supply: 0% (auxiliary air hoods are not allowed)
Static pressure loss: Not more than 124 Pa (0.5 in. wg) at 0.51 m/s (100 fpm) face velocity.
Auxiliary air-type fume hoods shall NOT be used in any NIH facilities. In the event of a retro-fit application, the
A/E shall investigate the capacities of the existing system exclusive of the auxiliary air, and laboratory supply and
exhaust system characteristics. Once it has been established that the system can support the addition or
replacement of an existing fume hood, this information shall be forwarded to the project officer for approval
before the design is allowed to proceed.
Cold rooms shall be provided with remote condensing units, which are not located directly above the room. Floor
mounted condensing units are preferred. Associated, air conditioning components shall be located to
accommodate service from outside the room. Condensing units shall be water cooled. If air cooled condensing
units are used due to the lack of hydronic cooling media, then, the temperature of the area surrounding the
condenser shall not be allowed to exceed 6C (10F) above the temperature of occupied adjacent areas. Design
consideration shall be given to ventilating and dissipating heat accumulation caused by equipment condensers.
Electrical rooms shall be ventilated to maintain a space temperature no more than 8C (14F) above outdoor air
temperature. Outdoor air into this room shall be filtered by using filters of 30% efficient filtration based on
ASHRAEs Standard 52, atmospheric dust-spot test efficiency. If the electrical room is located within the building,
and ventilation with outdoor air is not feasible, conditioned air shall be utilized to maintain the space to no more
than 26C (80F).
Secondary switchgear rooms shall be provided with heating and cooling equipment to maintain space
temperature between 18C (65F) and 26C (80F) and humidity level between 30 to 60% non-condensing to
protect switchgear and electronic controls. Switch-gear/transformer rooms located at the NIH Bethesda and
Poolesville campuses shall be provided with temperature and humidity sensors. These sensors shall be
connected to the existing Supervisory Control and Data Acquisition (SCADA) system, which is the energy
monitoring system for the campus.
Hydronic piping shall not be located with in electrical rooms and secondary switchgear rooms. In the event that
this can not be avoided, protection shall be added such as drip pans beneath all piping and equipment. These
drip pans shall be provided with water detection alarms connected to the BAS. Hydronic piping and drip pans
shall never be located over any electrical transformer, electrical panels, and switchgear.
Large equipment rooms, with significant ventilation requirements, shall be provided with multiple fans to avoid
having areas with excessive accumulated heat.
Boiler rooms and rooms with combustion equipment shall be provided with a ventilation system that combines
the ventilation requirements and the combustion air requirements.
Elevator machine rooms, telecommunication closets, fire alarm rooms, and other similar spaces with electronic
equipment may require air conditioning instead of outdoor ventilation. The A/E shall define criteria for these
spaces and design accordingly.
E.2 Redundancy:
Exhaust air systems shall be arranged with multiple manifolded fans designed to achieve N+1 redundancy and
maintain the exhaust air system fully operational, at all times. Each manifolded fan shall be designed to be fully
isolated while the overall system remains fully operational. In the case of single fan systems, in addition to the
main fan, a standby fan shall be provided. The A/E shall review redundancy requirements for each particular
system with the program user and the NIH/DOHS. Regardless of the system size, the following exhaust systems
shall be provided with an N+1 redundancy:
Isolation rooms
Laboratory general research areas
Fume hood exhaust
Radioisotope/radioactive fume hoods
Animal general research areas
Cage washers
Any other function as designated by NIH/DOHS
Redundancy Since research laboratories may conduct studies of long duration, which need to be performed
under consistent environmental conditions in order to achieve repeatable results, the failure of the HVAC system
is unacceptable. Central HVAC systems shall be provided with multiple air handling units and exhaust fans to
provide redundancy and improve reliability. These systems shall be designed to include manifolded air-handling
units to achieve N+1 redundancy and maintain operation at all times.
Summer 23 + 1 (73 + 2) 50 + 5
Winter 21 + 1 (70) + 2) 30 + 5
F.2. Laboratory Ventilation Rates:
Ventilation rate, for research laboratories, is typically driven by three factors: fume hood demand, cooling loads,
and removal of fumes and odors from the laboratory work area. The minimum outdoor air ventilation rate for
laboratory space is 6 air-changes per hour, regardless of space cooling load. This minimum ventilation rate shall
be maintained at all times. Some laboratories may require significantly higher ventilation rates to support fume
hood demand or to cool dissipated heat from laboratory instruments and equipment.
Air Filtration Air handling units to serve laboratory spaces shall be provided with filters up-stream the supply air
fans. These filters shall be 30% efficient pre-filters and 95% efficient after-filters. HEPA final filtration shall be
provided in AHU to serve special laboratories where research materials are particularly susceptible to
contamination from external sources. HEPA filtration of the supply air shall only be considered necessary for
critical applications. It is preferred that BSCs, which include HEPA filtration, be used rather than providing HEPA
filtration for the entire room. The A/E shall confirm with NIH/DTR and NIH/DOHS for the need of HEPA filtration
in laboratories.
Laboratory spaces shall remain at a negative air pressure in relation to corridors and other non-laboratory
spaces. Typically, these systems are designed to maintain 47 L/s (100 cfm) air flow from the corridor into each
lab module. Administration areas in laboratory buildings shall always be positive with respect to corridors and
laboratories. Supply air distribution for corridors shall be sized to offset transfer air to laboratories while
maintaining an overall positive building pressure.
Amount of supply air flow to laboratory spaces is to meet the cooling loads requirements as well as the exhaust
air requirements. Typically, the exhaust airflow requirements would exceed the cooling loads requirements. In
these situations, the supply air flow would need to be increased to makeup the difference between the cooling air
flow and the required exhaust air flow. In cases where the cooling load airflow requirements exceed the required
exhaust air rate requirements, supplemental cooling units may be required.
Special laboratories such as, genome DNA processing rooms, tissue culture laboratories, clean laboratories, etc,
may require a different type of relative room pressurization. Some special laboratories may require positive air
pressure in relation to adjacent spaces. In these cases, the use of a personnel entry or anterooms shall be
used. These special applications need to be reviewed by NIH/DTR and NIH/DOHS.
Loading docks and receiving areas shall be maintained as positive to the outdoors and negative to the building to
prevent the infiltration of vehicle fumes.
Redundancy Since most animal studies are of long duration, they shall be performed under consistent
environmental conditions in order to achieve repeatable results. Thus, the failure of the HVAC system is
unacceptable. Central HVAC systems shall be provided with multiple air handling units and exhaust fans to
provide redundancy and improve reliability.
Some rooms may be designated as hooded rack type rooms having a housing chamber with sash fronts similar
to a walk-in fume hood or individual air recycle systems of the laminar-flow type. Unit directional flow, laminar-
flow type systems for any of these rooms may also be required.
Summer 23 + 1 (73 + 2) 50 + 5
Winter 21 + 1 (70 + 2) 30 + 5
Ideally, all animal-holding rooms shall be capable of housing all type of species. The HVAC system shall also be
capable of maintaining the full range of requirements for all anticipated animal populations. The temperature
range required to accommodate most commonly used research animals is 18C (65F) to 29C (84F) controlled
to plus or minus 1C (2F). The ranges do not represent acceptable fluctuation ranges. The humidity shall be
between 30% and 70% and normally controlled to 50% plus or minus 5%. These ranges can be narrowed when
the species anticipated have similar requirements.
Animal-holding areas shall be maintained, at the design conditions, at all times. Design conditions shall be
satisfied under all load conditions between the various holding areas.
Some laboratories within the animal facility conduct special research requiring unique temperature and humidity
ranges and control. These special cases shall be evaluated and provided for on a case-by-case basis. The HVAC
system shall be designed to accommodate these unique conditions as they occur.
G.2 Ventilation Systems in Animal Research Facilities:
Ventilation systems in animal research facilities shall be designed in consideration of many factors such as:
Animal species and their population
Required minimum ventilation rate
Recommended ambient temperature and humidity
Heating and cooling loads within animal rooms
Heat gain produced by the animals
Use of microenvironments and the different ventilation methods in animal cages
Use of fume hoods and/or BSCs
Animal cage cleaning methods
Animal examinations method
Airborne contaminants
Institutional animal care standards, as applicable to animal facilities.
Ventilation rates, within each individual room, may vary depending of the actual animal specie in each room. The
following table shows a typical ventilation rates for various animal species:
Ventilation systems in animal research facilities shall meet the following requirements:
Rooms shall be designed to avoid drafts which could adversely affect animal health
Reduce airborne animal hair and particulate count.
Minimum ventilation rate for animal housing and treatment facilities shall be in accordance with ASHRAE HVAC
Applications Handbook, chapter Laboratories, ASHRAE Fundamentals Handbook, chapter Environmental
Control for Animals and Plants, and the Institute of Laboratory Animal Resources "Guide for the Care and Use of
Laboratory Animals"
Air recirculation within animal facilities is prohibited.
Air filtration - In addition to the typical pre-filtration and filtration normally used in air-handling units, final filtration
is generally provided in air-handling units serving animal areas. This final filtration is to remove particulate and
other contaminants, which can be generated within the air-handling equipment itself. Filter efficiency of final
filters varies from 95% to 99.99% (HEPA). The Design Engineer shall review the specific Program of
Requirements to establish specific filtration criteria.
G.2.a Microenvironments:
Ventilation rates in animal facilities are typically 10 to 15 outdoor-air changes per hour (ACH). This practice has
also been used for secondary enclosures (animal cages/microenvironments) and is considered to be an
acceptable general practice. Although it is effective in many animal-housing settings, this practice does not take
into account the range of possible heat loads; species, size and number of animals involved; type of bedding or
frequency of cage-changing; the room dimensions; or the efficiency of air distribution from the secondary to the
primary enclosure (animal room). In some situations, high flow rates may over ventilate a secondary enclosure
that contains few animals and waste energy or by under ventilating, a secondary enclosure that contains many
animals, which would allow heat and odor to accumulate.
For additional information, refer to ASHRAE, 2007, HVAC Applications handbook and to the Institute of
Laboratory Animal Resources, NRC, 1996, Guide for the Care and Use of Laboratory Animals.
System connections to microenvironments shall be designed to maintain manufacturers specified criteria. For
rooms housing animal ventilated racks, it is recommended, that the ventilation system be sized by adding the
airflow required for the animal cooling/heating loads, of fully loaded ventilated racks, to the airflow required for
other room cooling/heating loads such as lights, people, equipment, etc. This airflow shall be compared with the
manufacturers recommended airflow and the larger airflow amount shall be used. The A/E shall evaluate all
anticipated combinations of animals and cage systems; calculate supply air demands for make-up air, ventilation
rates, cooling demand and heating demand; and design for whichever criteria results in the highest airflow
demand.
Exhaust air from animal rooms shall be discharged outdoors without recirculation into any other room. For
protection of personnel and to minimize the potential for cross contamination of animals, the direction of airflow
shall be inward to the animal rooms, at all times. Where protection of the animals from possible contamination is
required, consideration should be made of providing ventilated airlocks for the animal rooms. The use of filtered
isolation cages may also be considered. Architect/Engineers should consult with animal facility personnel with
regard to the specific requirements for protection of animals.
In cage wash facilities, the dirty, clean, and cage washing equipment, including associated mechanical
supporting equipment area, shall be physically separated from each other, including equipment pits.
Some areas have special pressurization requirements and shall be addressed individually. By NIH/DTR and
NIH/DOHS
Animal-holding areas for transgenic or immunosuppressed populations shall be maintained at a positive pressure
and may require special filtration of supply air. When these rooms are maintained at positive pressure, an
anteroom or similar feature shall be placed between the animal room and the corridor.
Potentially infectious populations shall be maintained at a negative pressure to prevent contamination of other
animal populations. Depending on the nature of the infectious agents involved in the research, these areas may
be required to meet the design criteria for biohazard containment facilities. The use of anterooms or
microisolator housing units may be required to maintain these special conditions.
The pressure relationships for animal care areas including treatment rooms, procedure rooms, necropsy rooms,
and surgical areas require investigation by the design team with the facility user to determine project-specific
requirements. The HVAC system shall be adaptable so that pressure relationships can be modified as required
over the life of the facility. These applications need to be reviewed by NIH/DTR and NIH/DOHS
Dirty elevator shafts shall have negative air pressurization in relation to all surrounding areas.
B. Occupancy Loads:
The A/E shall base HVAC load calculations on the expected occupancy in each space and the activity level as
per ASHRAE Fundamentals Handbook.
Rat 90 5 450
Rabbit 8 5 40
Cat 8 5 40
Nonhuman primate 8 5 40
C. Laboratory Equipment Cooling Loads:
The central HVAC system shall provide, as a minimum, a cooling capacity for 1,892 W (6,455 BTUH) (sensible
heat) for laboratory equipment in a typical 22 m2 (237 ft2) laboratory module or cooling for the actual calculated
load, whichever is greater. The A/E shall make a detailed and complete inventory of all laboratory equipment
scheduled for installation in each space and determine the projected equipment load requirement using
estimated utilization factors. Equipment utilization factors shall be indicated in the Basis of Design report. The
A/E shall evaluate the following rooms used for laboratory support, often having higher than normal cooling
loads, as well as evaluate the use of supplemental cooling units to remove excessive sensible loads affecting
these areas, while maintaining minimum ventilation requirements:
Common equipment rooms.
Autoclave rooms.
Clean and dirty cage wash rooms.
Glassware washing rooms.
Darkrooms.
Special function rooms.
Electron microscope.
C. Lighting Loads:
Lighting loads shall not exceed the values listed below. The A/E shall base HVAC load calculations on actual
lighting loads.
Notes:
(1) Electrical power for room lighting load does not include electrical power for task lights
The Architect and Engineer (A/E) will be alerted to this type of situation and make an evaluation early in the
design stage to determine the feasibility of implementing the latest standard. The A/E should document such
findings, provide recommendations, and report them to the Project Officer for a decision on how to proceed.
The A/E design firm should use and comply with, as a minimum, the latest issue of the following design and
safety guidelines. In addition, the A/E should use other safety guidelines received from the NIH Project Officer or
as required by program. The A/E should utilize the latest versions of guidelines available at the time the project
proceeds with schematic design.
The design and safety guidelines include but are not limited to the following:
The International Building Code
The International Mechanical Code
The International Energy Conservation Code
National Fire Codes, all volumes National Fire Protection Association, (NFPA), 1 Bat-terymarch Park, Quincy,
MA 02269-9101.
ASHRAE Handbooks and Standards American Society of Heating, Refrigerating, and AirConditioning
Engineers (ASHRAE), Inc.: 1791 Tullie Circle, N.E., Atlanta, GA 30329.
Industrial Ventilation: A Manual of Recommended Practice for Design, American Conference of Government
Industrial Hygienist (ACGIH), 1330 Kemper Meadow Drive, Cincinnati, Ohio, 45240
Occupational Safety and Health Standards, CFR 29, Part 1910 U.S. Department of Labor, Occupational Safety
and Health Administration, (OSHA)
Biosafety in Microbiological and Biomedical Laboratories. U.S. Department of Health and Human Services.
Centers for Disease Control and Prevention and the National Institutes of Health. Washington, DC.
Primary Containment for Biohazards: Selection, Installation and Use of Biological Safety cabinets. U.S.
Department of Health and Human Services. Centers for Disease Control and Prevention and the National
Institutes of Health. Washington, DC.
Guide for the Care and Use of Laboratory Animals. Institute of laboratory Animal Resources, Commission on
Life Science, National Research Council. National Academy Press, 2101 Constitution Avenue, NW, Washington,
DC 20418
Guidelines for Design and Construction of Health Care Facilities. The American Institute of Architects Academy
of Architecture for Health with the assistance from the U.S. Department of Health and Human Services. The
American Institute of Architects, 1735 New York Avenue, NW, Washington, DC 20006.
Standard NSF/ANSI 49. Class II (laminar flow) biosafety cabinetry. National Sanitation Foundation (NSF). 789
N. Dixboro Road, Ann Arbor, MI 48105
Guidelines for Laboratory Design: Health and Safety Considerations, L.J. DiBerardinis, J.S. Baum, M.W. First,
G.T. Gatwood, and A.K. Seth. John Wiley & Sons, Inc, 111 River Street, Hoboken, NJ 07030-5774
Analysis of Air Supply Type and Exhaust Location in Laboratory Animal Research Facilities Using CFD, Andrew
Manning, Ph.D., Farhad Memarzadeh, Ph.D., P.E., Gerald Riskowski, Ph.D., P.E., ASHRAE Transactions 2000,
Volume 106, pt 1, DA-00-14-3, Pages 877-883
Thermal Comfort, Uniformity, and Ventilation Effectiveness in Patient Rooms: Performance Assessment Using
Ventilation Indices, Farhad Memarzadeh, Ph.D., P.E., Andrew Manning, Ph.D., ASHRAE Transactions 2000,
Volume 106, pt 2, MN-00-11-3, Pages 748-761
Methodology for Optimization of Laboratory Hood Containment Volumes I and II, November 1996, Farhad
Memarzadeh, PhD, P.E., NIH Office of the Director, ORF Publication , Bethesda, MD
Handbook: Assessing the Efficacy of Ultraviolet Germicidal Irradiation and Ventilation in Removing
Mycobacterium Tuberculosis, September 2000, Farhad Memarzadeh, PhD, P.E., NIH - Office of the Director,
ORF Publication, Bethesda, MD
In addition, refer to the following documents by using the associated internet links:
Guidelines for Research Involving Recombinant DNA Molecules. U.S. Department of Health and Human
Services, U.S. Public Health Service, National Institutes of Health, Bethesda, MD
NIH Guidelines for Research Involving Recombinant DNA Molecules
Laboratory Safety Monograph A Supplement to the NIH Guidelines for Recombinant DNA Research. U.S.
Department of Health and Human Services, U.S. Public Health Service, National Institutes of Health, Bethesda,
MD.
NIH Laboratory Safety Monograph: A Supplement to the NIH Guidelines for Recombinant DNA Research
A. HVAC Documents:
HVAC Documents shall comply with the requirements listed in Appendix B Architect-Engineer (A/E) Checklist of
Services as stated for the different phases of the particular project. Projects phases may vary from project to
project. Typically, project phases include: Pre-design, Schematic, Design Development, Construction
Documents, and Construction Administration.
B. Pressurization Analysis:
A room by room pressurization analysis shall be prepared by the A/E and submitted at the design development
phase to demonstrate pressurization relationships of adjacent areas. A schematic room layout shall be prepared
and submitted with the pressurization analysis to graphically demonstrate anticipated direction of airflows
between areas to be kept under positive pressure and negative pressure differentials in containment spaces.
The design documents shall include a room-by-room air balancing schedule to numerically identify supply air
quantity, exhaust air quantity and offset.
C. Contractors Requirements:
Project construction specifications shall require the contractor to provide the following:
Contractor shall provide startup, testing and operation verification for all equipment provided in the project.
Contractor shall provide owner training for all equipment provided in the project. This shall include testing,
operation and maintenance. Training shall be video taped for future training sessions. Copy of the DVD shall be
turn into the Project Officer.
Contractor shall provide complete Operation & Maintenance (O&M) manuals for all equipment provided in the
project. This shall include copies of all equipment related shop drawings, and copy of all warranties and
guarantees with the appropriate contact information. An electronic copy, CD or DVD format, shall also be
provided. Scanned copies are not acceptable.
E. Renovation Projects:
Renovation projects vary in size and scope. Complexity of renovation projects may also vary depending on the
location and the type of functions in the adjacent occupied areas. In order to minimize the impact to research in
adjacent spaces, maintain the safety of staff working in adjacent spaces as well as the public in general, avoid
unnecessary shutdowns, and avoid contamination of ongoing research and spaces a number of precautions shall
be implemented. The A/E shall include, in the construction documents, a proposed construction plan that is to
include measures to be implemented by the contractor. In addition, the contractor is to be required to further
develop the construction plan and submit it to the Project Officer for review and approval. Some of the
construction measures include but are not limited to:
All lab waste is to be removed and the space decontamination process must be completed prior to any
demolition work.
Dust-proof / fire-rated barriers shall be placed prior to any demolition work. These barriers may include the use
of fire-rated plastic sheeting, entry vestibules, and gasketed doors.
Seal all wall penetrations into the construction area.
Establish entering and exiting pathways and procedures
Debris shall be removed in covered carts
Exterior windows shall be sealed to reduce infiltration
Air from areas being renovated shall not be recirculated to the building airconditioning system.
Area being renovated shall remain under negative pressure in relation to the surrounding areas.
Core drilling and vibration producing activities shall be deferred to unoccupied periods
Use of sticky floor mats.
Routinely clean up shall include HEPA vacuuming. This may also include routinely floor mopping. This may be
required multiple times a day.
All Laboratory space renovation shall be reviewed by DOHS and the Division of Radiation Safety (DRS)