- Pengamatan

Uji kesesuaian sistem

Penyuntikan wakturetensi Luas area

1 3,533 37616698
2 3,487 36007307
3 3,450 37461217
4 3,423 36238879
5 3,407 37108957
6 3,397 38573765
7 3,393 37525016
Rata-rata 3,441428571 37218834,1
Sd 0,052248946 874339,097
Sbr / rsd 1,51823422 234918454

Larutan uji

Waktu retensi = 3,340

Luas area = 39998838

Kurva kalibrasi

Konsentrasi (ppm) Luas area

10,052 10324169
20,104 17175404
30,156 24145004
40,208 32652412
50,260 40047908
60,312 47786599

a = 2244875,8

b = 751626,036

r = 0,9995273

- Perhitungan
 Fase gerak = air : metanol (3:1)

3
Air
x 200 ml = 150 ml
3+1

1
Metanol = 3+ 1 x 200 ml = 50 ml

Pengenceran baku penbanding

Konsentrasi = 25,13 mg/50 ml = 0,5026 mg/ml 502,6 g/ml (ppm)

Pengenceran :

V1 . N1 =V2 . N2

502,6 ppm . 1 ml =N2 . 10 ml

502,6 ppm
N2 = 10 mL = 50,26 ppm

Bahan baku parasetamol

Konsentrasi = 25,13 mg/50 ml = 0,5026 mg/ml 502,6 g/ml (ppm)

Pengenceran :

N1 . V1 = N2. V2

502,6 ppm . 1 ml = N2 . 10 ml

502,6 ppm
N2 = 10 mL = 50,26 ppm

Perhitungan pengenceran analisis kuantitatif

Baku pembanding

0,2 mL V1 . N1 = V2 . N2

0,2 mL . 502,6 ppm = 10 mL . N2

100,52
N2 = 10 mL = 10,052 ppm
0,4 mL V1 . N1 = V2 . N2

0,4 mL . 502,6 ppm = 10 mL . N2

201,04
N2 = 10 mL = 20,104 ppm

0,6 mL V1 . N1 = V2 . N2

0,6 mL . 502,6 ppm = 10 mL . N2

301,56
N2 = 10 mL = 30,156 ppm

0,8 mL V1 . N1 = V2 . N2

0,8 mL . 502,6 ppm = 10 mL . N2

402,08
N2 = 10 mL = 40,208 ppm

1 mL V1 . N1 = V2 . N2

1 mL . 502,6 ppm = 10 mL . N2

502,6
N2 = 10 mL = 50,26 ppm

1,2 mL V1 . N1 = V2 . N2

1,2 mL . 502,6 ppm = 10 mL . N2

603,12
N2 = 10 mL = 60,312 ppm

Cara kurva kalibrasi

a = 2244875,8

b = 751626,036

r = 0,9995273
 y = bx + a

39998838 =751626,036 x + 2244875,8

399988382244875,8
x= 751626,037 = 50,2297 ppm

50,2297 ppm
% kadar = 50,24 ppm x 100% = 99,9794 %

Kurva kalibrasi PCT

60000000

50000000

40000000

30000000
Luas area Kurva kalibrasi PCT
20000000

10000000

Konsentrasi (ppm)

Cara one point

Lu
Cu = Ls x Cs

39998838
= 40047908 x 50,25 ppm = 50,1984 ppm
 50,1984 ppm
% kadar = 50,24 ppm x 100 % = 99,9172 %