ADLN- PERPUSTAKAAN UNIVERSITAS AIRLANGGA

SUMMARY

The Effect of Limestone Dust Exposure Against Increased Blood Serum TNF
and Declining The lung Function On Miners In Jadi Village Semanding
District Tuban Regency

The characteristics of dust particle, exposure length, amount and

respiratory response to dust particle are the main factor of pathogenesis
pneumoconiosis. A test has done to examine the function of the lungs which aims
to support diagnose on obstructive lungs disease and as a distinguishing between
obstructive and restrictive pulmonary ventilation disorder, which obstructive
disorder influence the expiration ability whereas restrictive disorder influence the
inspiration ability. There is inflammation mediator that has vital role in
pathogenesis pneumoconiosis, it is Tumor Necrosis Factor (TNF)- , which can
still be a strong indicator in estimating prognosis pneumoconiosis on a person
after this research accomplished. Non-specific defense mechanism reaction can
occur to workers in case they inhale the dust, such as sneezing, coughing,
mucociller transport disorder and fagochitosis by macrofag. Respiratory
constriction will occur because of there is stimulation on smooth muscle around
respiratory duct which is caused by excessive dust degree. The aims of this study
was limestone dust exposure on the increasing of the blood serum TNF and
declining lung function on the miners.
This analytical research method was observational, however according to
the time of research, the method was longitudinal prospective study. It was done
on limestone mine in Jadi village Semanding district Tuban regency for 3 months.
Population of this research was the limestone miners in Jadi village Semanding
district Tuban regency and the sample of this research was divided into exposed
group and non-exposed group, exposed group consisted of 7 limestone miners in
village Jadi and non-exposed group consisted of 6 office employees village Jadi
Semanding district Tuban regency. Data collecting technique uses interview,
measuring total amount dust in working environment, measuring the personal
dust, taking blood sample and spirometry. Blood serum TNF and the function of
lungs were measured before and after working. Free variable in this research was
personal dust degree of limestone miners, whereas the dependent variable was
blood serum TNF degree and the function of the lungs. Age, working period, and
smoked habit were the intruder variable. Personal limestone dust measuring
method uses Personal Dust Sampler (PDS). Meanwhile, examining blood serum
TNF used ELISA technique and to measure the functions of the lungs (FVC and
FEV1) used spirometer (Spirolab III). Data analyses analytically. A data analysis
inferentially used Multiple Regression Test.

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TESIS Dampak Paparan Debu .... Damayanti Sima Sima Sohilauw

 ADLN- PERPUSTAKAAN UNIVERSITAS AIRLANGGA

The result of this research showed that the mean age of the worker was 36-
40 years old, limestone miners (42,8%), office employees (66,6%), the mean of
working period of miners was 4,43 years and 2,83 years for office employee,
85,7% limestone miners was a light smokers, while all of the office employees
were light smokers. The mean of personal dust degree miners after working for 8
hours was 10,407 mg/m3 and office employee was 0,265 mg/m3. The increasing
of blood serum TNF on miners was 71,43% and on office employees was 16,7%
with mean for miners (2,51) and office employees (-9,82). The decreasing of FVC
and FEV1 on miners were 85,7% and 71,4% while FVC and FEV1 on office
employees were 50% and 16,7%. Personal limestone dust did not influence
significantly to the increasing of TNF (multiple regression test, p>0,05), in the
other hand, personal limestone dust influenced to the declining the lungs function
of the miners (multiple regression test, p<0,05). Age, working period and smoked
habit neither influenced to the increasing of TNF nor the decreasing of the
function of the lungs.
It is conclusion that personal limestone dust does not influence to the
increasing of blood serum TNF, but personal limestone dust influence to the
declining the lungs function of the miners after working. Suggestion: do regular
check up on the function of the lungs is important, especially to detect short term
change which occur and can help to identify risky subject.

TESIS Dampak Paparan Debu .... Damayanti Sima Sima Sohilauw