a.

Perhitungan hasil optimasi

Perhitungan Optimasi untuk Metanol : Air (30 : 70) dengan laju alir 0,75 mL/menit
Perhitungan pengenceran dari 100 ppm menjadi 20 ppm

100 v =20 ppm 5 ml

100
v= =1 ml
100

1 ml dari yang 100 ml + 4 ml pengencer

Larutan Rt AUC
Baku 1.1 9.627 0.8369
Baku 2.1 9.613 0.8393
Baku 3.1 9.680 0.8153
Rata-rata 9.64 0.8305

Larutan Rt AUC
Baku 1.2 10.887 0.8265
Baku 2.2 10.873 0.8641
Baku 3.2 10.973 0.8538
Rata-rata 10.911 0.8481

SD1 - Rt
(x - xi) (x xi)2
(9.64 - 9.627) = 0.013 0.000168
(9.64 9.613) = 0.027 0.000729
(9.64 9.680) = -0.04 0.0016
Jumlah 0.002497

2
( xxi )
SD =
( n1 )
 SD =
0.002497
2
=0.0353

0.0353 100
%SDR= =0.366
9.64

SD2 - Rt
(x - xi) (x xi)2
(10.911 - 10.887) = 0.024 0.000576
(10.911 10.873) = 0.038 0.001444
(10.911 10.973) = -0.062 0.003844
Jumlah 0.005864

2
( xxi )
SD =
( n1 )

SD=
0.005864
2
=0.054

0.054 100
%SDR= =0.496
10.911

AUC1
(x - xi) (x xi)2
(0.8305 0.8369) = 0.0064 0.00004096
(0.8305 0.8393) = -0.0088 0.00007744
(0.8305 0.8153) = 0.0152 0.00023104
Jumlah 0.00034944

2
( xxi )
SD =
( n1 )

SD=
0.00034944
2
=0.013218

0.013218 100
%SDR= =1.592
0.8305
AUC2
(x - xi) (x xi)2
(0.848 0.8263) = 0.0215 0.00046225
(0.848 0.8641) = -0.0161 0.00025921
(0.848 0.8538) = -0.0058 0.00003364
Jumlah 0.0007551

2
( xxi )
SD =
( n1 )

SD=
0.0007551
2
=0.01943

0.01943 100
%SDR= =2.291
0.848