Objectives
1. To study the role of cytokinins in leaf senescence
Materials
Plant, 3 cork borer, paper towel, deionized water, 5 petri dishes, N6-
benzyladenine (BAP), screw-top test tube, aluminium foil, gooseneck lamp,
freezer, ethanol, spectrophotometer
Procedure:
1. The plant was prepared to be used in this experiment
2. By using 3 cork borer, 35 40 discs were cut from two primary leaves. The
primary leaves are removed from the plant and layed topside down on
several layers of paper towel. Cork borer was pressed down firmly and
evenly on the desired are of the leaves.
3. Then, it was placed into 400ml beaker containing about 200ml of
deionized water
4. 5 petri dishes are obtained and numbered from 1 -5. 15ml of deionized
water are added to each of dishes 1 and 2. 15 ml of N6-
benzyladenine(BAP) solution at 1.3 10-4 M, 1.3 10-5 M or 1.3
10-6 M are added into dish 3,4,5 respectively
5. 5 leaf discs are transferred by using a spatula or forceps from the beaker
to Kimwipe tissue. 5 dry discs are blotted gently with tissues, and then
being measure and recorded in Table 1. After being weighted, 5 discs are
placed adaxial side up on the solution in dish number 1
6. 5 dry disc are selected and 5 discs are weighed for each of the other
dishes in the number 2-5
7. Another 5 leaf discs are selected, dried and weighed and then being
dropped into an empty screw-top test tube labelled 6 initial. The tube is
closed, wrapped with aluminium foil and stored in freezer until we are able
to harvest other leaf disc from the petri dishes
8. Then the discs in the petri dishes are incubated about 25 oC
9. The discs are examined after 2 days of incubation and any visible
differences will be observed. Then incubation process is being continued
as before