NAMA : DITA NURHALIMAH (1407114638)

LILIS SURIANI (1407113270)

MAHATHIR NUR MUHAMMAD (1407119411)
IRFAN SETIAWAN (1407114829)
UAS : BIOREAKTOR

1. Produksi protein single-sell dari heksadekana diberikan pada persamaan reaksi

berikut:

C16H34 + aO2 + bNH3 cCN1.66 O0.27 N0.2 + dCO2 + eH2O

Dengan cCN1.66 O0.27 N0.2 mewakili biomassanya. Diberikan bahwa RO = d/a =

0.43, tentukanlah koefisien stoikiometrinya a, b, c, d, dan e.

Penyelesaian :

d

d = 0.43 a a 0.43

C: 16 = c + d 16 c=d

H: 34 + 3(0.2)c = 1.66c + 2e
34 = 1.66 + 2e
2( 16c)
O: = 0.27c + 2(16 c) + e
0.43

74.42 4.65c = 0.27c + 32 2c + e

42.42 = 2.92c + e

34=1.06 c+ 2 e|x 1|34=1.06 c +2 e

42.42 = 2.92c + e |x 2| 84.84 = 5.84 + 2e

50.84 = 4.78 c

c = 10.636
e = 42.42 (10.636) = 6.36

1.66 ( 10.636 )+2 ( 11.363 ) 34

b =[
3

= 2.1273

d = 16 10.636

= 5.364

5.364
a = 0.43

= 12.474

7.3. Pertumbuhan S.cerevisiae pada glukosa dibawah kondisi anaerobik dan

dideskripsikan oleh reaksi keseluruhan.

C6H12O6 + NH 0.59 CH1.74N0.2O0.45 (biomass) + 1.54 CO2 +

3

1.3C2H5OH + 0.036 H2O

a. Hitunglah koefisien yield biomassa Yx/s.

YC
b. hitunglah koefisien yield produk YE+OH/S, YCO2/S, 3 H8 O3 s

c. hitunglah koefisien .

Penyelesaian:

a. MW biomass = 12 + 1.74 + 0.2(14) + 0.45(16) = 23.74

MW substrat = 6(12) + 12 + 6(16) = 180
0.59 ( MW ) Biomass

Yx/s ( MW ) Substrate

0.59 ( 23.74 )

180

= 0.0778 g dwsubstrat/ g glucose

b. MW EtOH = 2(12) + 5 + 16 + 1= 46
1.3 ( MW of E t OH )

YEtOH/S ( MW Gl u cose )

1.3 ( 46 )
= ( 180 )

= 0.332 gdw EtOH/ g glucose

MW CO2 = 12 + 2(16) = 44
1.54 ( MW of CO2 )
CO2
Y /S ( MW Glucose )

1.54 ( 44 )
= ( 180 )

= 0.376 gdw CO2/ g glucose

MW C3H8O3 = 3(12) + 8(1) + 3(16) = 92

0.43 ( MW of CO 2 )
YC
( MW Glucose )
3 H8 O3

0.43 ( 92 )
= ( 180 )

= 0.2197 gdw C3H8O3 / g glucose

c. C6H12O6 + NH 0.59 CH1.74N0.2O0.45 (biomass) + 1.54 CO2 +

3

1.3C2H5OH + 0.036 H2O

MW = 0.59 + 0.43(3) + 1.54(1) + 1.3(2) = 6

=0.2 ( 0.59 )

= 0.118